Field Trials of an Inactivated Virus Vaccine Against Porcine Parvovirus

Castro, José María Albertos; Pozo, M. del; Simarro, I.

doi:10.1111/j.1439-0450.1992.tb01178.x

Cited by 3 publications

(2 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Small litters and PPV antibodies found in other gilts piglets' serum support this observation. Castro et al (1992) also found that 6% of gilts vaccinated twice remained seronegative until farrowing but they did not report reproductive performance Fig. 1.…”

Section: Discussionmentioning

confidence: 95%

Parvovirus Antibodies in Vaccinated Gilts in Field Conditions – Results with HI and ELISA tests

Oravainen¹,

Hakala²,

Rautiainen³

et al. 2006

Reprod Domestic Animals

View full text Add to dashboard Cite

This study was conducted to determine the antibody response for porcine parvovirus (PPV) of 39 gilts in field conditions after vaccination. Gilts from four herds endemically infected with PPV were injected twice with a commercial vaccine of inactivated PPV and Erysipelothrix rhusiopathiae. The PPV antibodies were analysed both with haemagglutination inhibition (HI) and enzyme-linked immunosorbent assay (ELISA) in order to study the agreement between these methods. The possible association between high-antibody titres and reproductive failure (repeat breeding, culling for infertility, < or = 6 piglets born alive) was also investigated. In these study herds, endemically infected by PPV, most gilts (84.6%) had not seroconverted by the age of 6 months. On-field vaccination resulted in a consistent increase of humoral immunity not exceeding the antibody level of 1 : 512 in the majority of gilts in all herds examined. The agreement between ELISA and HI tests was moderate (Spearman's rho = 0.87, kappa = 0.63). The seroconversion over the level >1:512 by mid-pregnancy was not associated with reproductive failure.

show abstract

Section: Discussionmentioning

confidence: 95%

Parvovirus Antibodies in Vaccinated Gilts in Field Conditions – Results with HI and ELISA tests

Oravainen¹,

Hakala²,

Rautiainen³

et al. 2006

Reprod Domestic Animals

View full text Add to dashboard Cite

show abstract

“…According to Sorensen et al (1988) the immune response depends on amount of inactivated virions presented in the vaccine. For example, Sorensen et al (1988) reported titres as high as 1:2048, Pye et al (1990) reported titres as high as 1:512, according to Castro et al (1992) titres can reach values over 1:640. Therefore in our survey we considered a pig to be infected with HI titres higher than 1:256 only in non-vaccinated herds.…”

Section: Discussionmentioning

confidence: 99%

Porcine Parvovirus Infection in Boars in the Czech Republic

Bican¹,

Svoboda²,

Drábek³

2002

Acta Vet. Brno

View full text Add to dashboard Cite

Boars may play a significant role in dissemination of parvovirus (PPV). During acute infection the virus is shed by various routes, including semen. The objective of this study was to evaluate occurrence of natural infection with porcine parvovirus in boars in the Czech Republic by serological examination. A total number of 800 sera from boars of 42 herds were collected. The sampling was made between January and December 1999. All serum samples were tested for porcine parvovirus (PPV) antibodies by haemagglutination inhibition test (HIT). In 31 herds, boars have never been vaccinated to PPV. In 11 herds boars were regularly vaccinated to PPV. A pig was considered infected with HI titres higher than 1:256 in non-vaccinated herds. In vaccinated herds non-infected boars could be determined only with titres equal to or lower than 1: 256. Infection with porcine parvovirus was detected in 80.6% of non-vaccinated herds. Non-infected boars were only found in 18.2% of vaccinated herds. From the total number of 528 boars tested in nonvaccinated herds 60.8% were detected as infected. From the total number of 272 boars tested in vaccinated herds 37.5% were found to be non-infected. Our results indicate that infection with porcine parvovirus is widespread in boars in the Czech Republic. With regard to the possibility of excretion of parvovirus in semen it is obvious that boars can play an important role in transmission of parvovirus in pig herds in the Czech Republic.

show abstract

Untitled

Belák

Rivera

Ballagi-Pordány

et al. 1998

Veterinary Research Communications

View full text Add to dashboard Cite

To estimate the potency of a porcine parvovirus (PPV) vaccine, three vaccinated and three non-vaccinated pregnant gilts were infected with PPV and the distribution of the virus was studied in the tissues of their 51 fetuses. Virus detection was attempted using haemagglutination (HA) and immunofluorescence (IF) assays, as well as by standard (single) and nested polymerase chain reactions (PCR). None of the detection methods yielded positive results when used to test for the presence of virus in suspensions of organs from the fetuses from the vaccinated gilts. However, the virus was detected in the fetuses from non-vaccinated gilts as follows: HA was positive in 14 cases out of 23 (60.8%), IF in 16/23 (69.5%), standard PCR in 12/20 (60%), and the nested PCR in 19/23 (82.6%). Although the correlation among the results of various methods of virus detection was rather close (r < 0.83), the sensitivity of the nested PCR was the highest, both when testing dilutions of PPV and when analysing the fetal organs. The nested PCR therefore provides a reliable approach for studies of virus distribution in fetal organs, with special reference to potency tests on vaccines.

show abstract

Field Trials of an Inactivated Virus Vaccine Against Porcine Parvovirus

Cited by 3 publications

References 9 publications

Parvovirus Antibodies in Vaccinated Gilts in Field Conditions – Results with HI and ELISA tests

Parvovirus Antibodies in Vaccinated Gilts in Field Conditions – Results with HI and ELISA tests

Porcine Parvovirus Infection in Boars in the Czech Republic

Untitled

Contact Info

Product

Resources

About