Field Trial in Chiapas, Mexico, of a Rapid Detection Method for Malaria in Anopheline Vectors with Low Infection Rates

Ramsey, Janine M.; Bown, D. N.; Aron, Jordan; Beaudoin, R. L.; Méndez, Jorge Fernando

doi:10.4269/ajtmh.1986.35.234

Cited by 7 publications

(2 citation statements)

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“…We have no robust evidence whether the mosquitoes in Minami Daito Island had been introduced such as in Hawaii or not, however, the first Japanese migrants to this desert island in 1900 had suffered from mosquito bites [22], suggesting that some mosquito species originally distributed in this island. It is reported that the infection rates of human malaria in wild mosquitoes are generally less than 10% in most endemic areas by direct detections of sporozoites and/ or oocysts from vectors [33,42]. Therefore, lower infection rates in the vectors in Minami Daito Island (1.2%) might associate with their origin compared to the artificially introduced vector in Hawaii (15%).…”

Section: Discussionmentioning

confidence: 99%

Detection of Avian Plasmodium spp. DNA Sequences from Mosquitoes Captured in Minami Daito Island of Japan

Ejiri

Sato

Sasaki

et al. 2008

The Journal of Veterinary Medical Science

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ABSTRACT. Several species of birds in Minami Daito Island, an oceanic island located in the far south from the main islands of Japan, were found to be infected with avian Plasmodium. However, no vector species of the avian malaria in this island have been revealed yet. To speculate potential vectors, we collected mosquitoes there and investigated using a PCR procedure whether the mosquitoes harbor avian malaria or not. Totally 1,264 mosquitoes including 9 species were collected during March 2006 to February 2007. The mosquitoes collected were stored every species, sampled date and location for DNA extraction. Fifteen out of 399 DNA samples showed positive for the partial mtDNA cytb gene of avian Plasmodium. Estimated minimum infection rate among collected mosquitoes was 1.2% in this study. Four species of mosquitoes; Aedes albopictus, Culex quinquefasciatus, Lutzia fuscanus and Mansonia sp. had avian Plasmodium gene sequences. Detected DNA sequences from A. albopictus and L. fuscanus were identical to an avian Plasmodium lineage detected in bull-headed shrike (Lanius bucephalus) captured in the island. Different sequences were detected from C. quinquefasciatus, which were corresponding to an avian Plasmodium from a sparrow (Passer montanus) and Plasmodium gallinaceum. Our results suggest that A. albopictus, Lutzia fuscanus, C. quinquefasciatus, and Mansonia sp. could be potential vectors of avian malaria in Minami Daito Island. This study was the first report of molecular detection of avian Plasmodium from mosquitoes in Japan.

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Section: Discussionmentioning

confidence: 99%

Detection of Avian Plasmodium spp. DNA Sequences from Mosquitoes Captured in Minami Daito Island of Japan

Ejiri

Sato

Sasaki

et al. 2008

The Journal of Veterinary Medical Science

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“…PCR products were subsequently sequenced in both directions using BigDye™ terminator mix (Applied Biosystems, Foster City, CA, USA). Phylogenetic analyses using about 433-bp sequences were performed as described previously (Ramsey et al 1986). Briefly, using the neighbor-joining (NJ) method by PAUP program, the Kimura two-parameter model was utilized to estimate the evolutionary distances.…”

Section: Methodsmentioning

confidence: 99%

Prevalence of avian malaria parasite in mosquitoes collected at a zoological garden in Japan

et al. 2009

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Several species of captive birds at zoological gardens of Japan were found to be infected with avian Plasmodium. However, incriminated vector mosquito species have not been identified yet. To indicate the competent vectors of avian malaria parasite, we collected mosquitoes at a zoological garden in Japan and examined for the avian malaria parasite DNA. Totally, 1,361 mosquitoes of 11 species were collected in the zoological garden of Kanagawa, the south of Tokyo in Japan in 2005. Captured mosquitoes were pooled by each species, date collected, and location and used for DNA extraction. Eight out of 169 DNA samples were positive for the nested PCR of avian Plasmodium cyt b gene. Estimated minimum infection rates of mosquitoes were 5.9 per 1,000. The PCR positive mosquito species were Culex pipiens group and Lutzia vorax. Some DNA sequences amplified from collected mosquitoes were identical to avian Plasmodium lineages detected from captive birds in the same zoological garden studied. Our results suggest that C. pipiens group and L. vorax could be incriminated vectors of avian malaria parasite transmitting in captive birds kept in the zoological garden in Japan.

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Detection of Malarial Parasites in Mosquitoes

Wirtz

1991

Advances in Disease Vector Research

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Field Trial in Chiapas, Mexico, of a Rapid Detection Method for Malaria in Anopheline Vectors with Low Infection Rates

Cited by 7 publications

References 0 publications

Detection of Avian Plasmodium spp. DNA Sequences from Mosquitoes Captured in Minami Daito Island of Japan

Detection of Avian Plasmodium spp. DNA Sequences from Mosquitoes Captured in Minami Daito Island of Japan

Prevalence of avian malaria parasite in mosquitoes collected at a zoological garden in Japan

Detection of Malarial Parasites in Mosquitoes

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