Field transcriptome analysis reveals a molecular mechanism for cassava-flowering in a mountainous environment in Southeast Asia

Tokunaga, Hiroki; Quynh, Do Thi Nhu; Anh, Nguyen Hai; Nhàn, Phạm Thị Thanh; Matsui, Atsushi; Takahashi, Satoshi; Tanaka, Maho; Anh, Ngo Minh; Đông, Nguyễn Văn; Hàm, Lê Huy; Higo, Asuka; Hoa, Truong Minh; Ishitani, Manabu; Minh, Nguyen Ba Nhat; Hy, Nguyen Huu; Srean, Pao; Thu, Vu Anh; Tung, Nguyen Ba; Vu, Nguyen Anh; Yamaguchi, Kaho; Tsuji, Hiroyuki; Utsumi, Yoshinori; Seki, Motoaki

doi:10.1007/s11103-020-01057-0

Cited by 14 publications

(19 citation statements)

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“…However, total plant growth, which is an indication of whole-plant photosynthesis, was only about 25% as much in Ubiaja than Ibadan, which challenges this hypothesis. In some plant systems, flowering is evoked by a prior water deficit [42,43], and studies of cassava have shown a correlation between the dry season in mountainous areas and flowering [44]. However, in the present study, better flowering in Ubiaja was associated with more rainfall than in Ibadan (Fig 1).…”

Section: Plos Onecontrasting

confidence: 66%

“…Adeyemo et al [11] previously showed that MeFT1 expression was related to flowering tendency, as it was expressed at higher levels in '0002 (early genotype) than in '419 (late, with a plastic phenotype). Studies of cassava in various locations in Vietnam have shown that in a mountainous region, flowering is stimulated during the dry season, and correspondingly, this is when expression of MeFT1 is highest [44]. FT (florigen) has been established in angiosperms as a mobile long distance signaling protein that is transcribed and translated in leaves, and the protein moves from leaf to the shoot apical meristem where it induces flowering [20,54].…”

Section: Plos Onementioning

confidence: 99%

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Environmental responsiveness of flowering time in cassava genotypes and associated transcriptome changes

et al. 2021

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Cassava is an important food security crop in tropical regions of the world. Cassava improvement by breeding is limited by its delayed and poor production of flowers, such that cassava flowering under field conditions indirectly lengthens the breeding cycle. By studying genotype and environment interaction under two Nigerian field conditions (Ubiaja and Ibadan) and three controlled temperature conditions (22°C/18°C, 28/24°C and 34/30°C (day/night)), we found that while early flowering genotypes flowered at similar times and rates under all growing conditions (unfavorable and favorable field and controlled-temperature environments), late flowering genotypes were environmentally sensitive such that they were substantially delayed in unfavorable environments. On the basis of nodes-to-flower, flowering of late genotypes approached the flowering time of early flowering genotypes under relatively cool Ubiaja field conditions and in growth chambers at 22°C, whereas warmer temperatures elicited a delaying effect. Analysis of transcriptomes from leaves of field and controlled-temperature environments revealed that conditions which promote early flowering in cassava have low expression of the flowering repressor gene TEMPRANILLO 1 (TEM1), before and after flowering. Expression data of field plants showed that the balance between flower stimulatory and inhibitory signaling appeared to correlate with flowering time across the environments and genotypes.

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Section: Plos Onecontrasting

confidence: 66%

Section: Plos Onementioning

confidence: 99%

Environmental responsiveness of flowering time in cassava genotypes and associated transcriptome changes

et al. 2021

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“…We analyzed this dataset and found that GI and FT expressions showed similar patterns (Supplementary Fig. 2), as indicated by Tokunaga et al (2020). We analyzed additional genes we identified in our study and provided expression heat map of these genes.…”

Section: Expression Patterns Of Flowering-time Genes In Field-grown Cassava Plantsmentioning

confidence: 66%

“…To support this finding, we compared the expression patterns of GI, FT and other flowering time genes in the field grown cassava in another location. Tokunaga et al (2020) reported the field transcriptome data of cassava grown in Vietnam.…”

Section: Expression Patterns Of Flowering-time Genes In Field-grown Cassava Plantsmentioning

confidence: 99%

Field-transcriptome analyses reveal developmental transitions during flowering in cassava (Manihot esculenta Crantz)

et al. 2021

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Key messageWe characterized genes that function in the photoperiodic flowering pathway in cassava. Transcriptome analysis of field-grown plants revealed characteristic expression patterns of these genes, demonstrating that fieldgrown cassava experiences two distinct developmental transitions. Abstract Cassava is an important crop for both edible and industrial purposes. Cassava develops storage roots that accumulate starch, providing an important source of staple food in tropical regions. To facilitate cassava breeding, it is important to elucidate how flowering is controlled. Several important genes that control flowering time have been identified in model plants; however, comprehensive characterization of these genes in cassava is still lacking. In this study, we identified genes encoding central flowering time regulators and examined these sequences for the presence or absence of conserved motifs. We found that cassava shares conserved genes for the photoperiodic flowering pathway, including florigen, anti-florigen and its associated transcription factor (GIGANTEA, CONSTANS, FLOWERING LOCUS T, CENTRORADIALIS/TERMI-NAL FLOWER1 and FD) and florigen downstream genes (SUPRESSOR OF OVEREXPRESSION OF CONSTANS1 and APETALA1/FRUITFUL). We conducted RNA-seq analysis of field-grown cassava plants and characterized the expression of flowering control genes. Finally, from the transcriptome analysis we identified two distinct developmental transitions that occur in field-grown cassava.

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“…The objectives of the ILCMB are to 1) establish a seed system to facilitate the adaptation of new variety, and 2) speed up variety development using new breeding techniques (NBT), such as genome editing, and 3) human capital development through establishing practical knowledge and technology transfer developed at CIAT-HQ in Colombia and elsewhere. Through the ILCMB, RIKEN contributed to bringing new molecular insights of cassava flowering by transcriptome analysis (Tokunaga et al 2020) and building capacity to deliver improved cassava varieties through molecular breeding approaches.…”

Section: Cassava Crop Developmentmentioning

confidence: 99%

Crop Development with Data-driven Approach towards Sustainable Agriculture: Lifting the Achievements and Opportunities of Collaborative Research between CIAT and Japan

Ogawa

Selvaraj

Ishitani

2021

JARQ

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International Center for Tropical Agriculture (CIAT) has a long history of collaboration with Japanese institutions to develop improved crop varieties and adopt agricultural technologies with pioneering approaches for farmers' benefit. Since 1972, Japan has enjoyed a long history of successful cooperation with the CGIAR (formerly the Consultative Group on International Agricultural Research) after becoming its 22nd Member to meet global challenges such as climate change affecting agriculture and food systems in diplomacy through Japan's advanced science and technology. This long-standing collaboration adopts agricultural technologies developed in Japan and promotes agricultural innovation through cutting-edge technologies such as remote sensing for Internet of Things (IoT) and Artificial Intelligence (AI) that will provide novel solutions to the agriculture and food sectors in the tropics. Nowadays, it is presumed that this approach to data-driven agriculture realization will help establish a sustainable agroecosystem with increased agricultural productivity and sustainability by adapting or mitigating the effects of climate change and efficient use of natural resources and establishing a sustainable food value chain. This review highlights the research collaborations and new opportunities on advanced breeding technologies and digital innovations towards data-driven agriculture. In conjunction, we present a potential collaborative research between CIAT and Japan, prioritizing the current and future challenges in agriculture and food systems.

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Field transcriptome analysis reveals a molecular mechanism for cassava-flowering in a mountainous environment in Southeast Asia

Cited by 14 publications

References 50 publications

Environmental responsiveness of flowering time in cassava genotypes and associated transcriptome changes

Environmental responsiveness of flowering time in cassava genotypes and associated transcriptome changes

Field-transcriptome analyses reveal developmental transitions during flowering in cassava (Manihot esculenta Crantz)

Crop Development with Data-driven Approach towards Sustainable Agriculture: Lifting the Achievements and Opportunities of Collaborative Research between CIAT and Japan

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