Field study on bovine paratuberculosis using real-time PCR and liquid culture for testing environmental and individual fecal samples implemented in dairy cow management

Schwalm, Anja; Metzger-Boddien, Christoph; Seemann, G.; Mandl, Josef; Obiegala, Anna; Pfeffer, Martin; Sting, Reinhard

doi:10.3168/jds.2018-15649

Cited by 5 publications

(2 citation statements)

References 32 publications

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“…Future experiments should also include feces collection and tests for paratuberculosis based on culture and PCR [ 30 , 31 , 32 ]. In recent field studies of dairy cows, fecal examination by real-time PCR testing of MAP-specific DNA was found to have 74% sensitivity of culture [ 33 ]. In recent studies, peptide magnetic separation PCR test (PMS-PCR) was found to obtain greater sensitivity and lower specificity than ELISA for paratuberculosis diagnosis [ 34 ], which indicates that this method has potential.…”

Section: Discussionmentioning

confidence: 99%

The Risk of False-Positive Serological Results for Paratuberculosis in Mycobacterium bovis-Infected Cattle

et al. 2021

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Both bovine tuberculosis (BTB) and paratuberculosis (paraTB) continue to cause significant economic losses in cattle breeding; in addition, their etiological agents have zoonotic potential. Although the diagnostics of both diseases are still being improved, problems still remain, such as the potential for cross-reactivity to the antigens used in tests. The aim of the present study was to confirm whether animals known to harbor Mycobacterium bovis antibodies are at increased risk of yielding positive results in paraTB serotesting and, additionally, to verify the accuracy of three commonly used methods for confirming M. bovis infection: ELISA, the tuberculin skin test (TST), and the presence of gross lesions. Material was collected from 98 dairy cattle suspected of BTB due to TST-positive results. During postmortem examination, gross lesions were assessed visually. Blood, lymph nodes, and TB-suspected organs were collected. Serum was obtained from the collected blood and tested serologically for TB and paraTB. The tissues underwent standard microbiological testing for M. tuberculosis complex. Among the 98 TST-positive individuals, tuberculous gross lesions were detected in 57 (58.1%), MTBC were isolated in 83 (84.7%), and the ELISA test was positive for 21 (21.4%). None of the lesions characteristic for paraTB were detected. The chance of obtaining a positive TB result by ELISA was seven times higher using the ELISA-paraTB method; hence, there is a significant risk of obtaining false-positive serological results for paraTB in M. bovis-infected cattle. However, the hypothesis that infection of M. bovis or prior TST performance may have boosted the host immune response and therefore increased the sensitivity of the paraTB-ELISA cannot be excluded.

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Section: Discussionmentioning

confidence: 99%

The Risk of False-Positive Serological Results for Paratuberculosis in Mycobacterium bovis-Infected Cattle

et al. 2021

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“…El cultivo en medio líquido a base de caldo Middlebrook 7H9 tiene mayor sensibilidad ARTÍCULOS ORIGINALES en comparación con el medio sólido, que es comúnmente utilizado. Los resultados son confiables y en la mitad del tiempo, con bajos costos, por lo que puede automatizarse en laboratorio (Schwalm et al, 2019) Por medio de la PCR se puede llegar a una especificidad alta, hasta del 60 % y una sensibilidad entre el 97 % y el 99 %. Sin embargo, su aplicabilidad es costosa y limitada.…”

Section: Métodos De Diagnósticounclassified

Paratuberculosis bovina, revisión de literatura

et al. 2021

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La paratuberculosis bovina (PTB) es una enfermedad crónica causada por el bacilo Mycobacterium avium subespecie paratuberculosis (MAP). Tiene distribución a nivel mundial y genera pérdidas económicas para los productores por el bajo rendimiento productivo de los animales y el amplio periodo de incubación, en el cual es excretada a través de la materia fecal sin signos clínicos. Así mismo, se reporta que los animales más susceptibles de contraer la enfermedad son los terneros. Entre los métodos de diagnóstico utilizados para la MAP, están PCR, ELISA, cultivo bacteriano de materia fecal, el examen para detección de MAP en muestras de tejido y la prueba de hipersensibilidad cutánea retardada Johnina intradérmica. La MAP es una bacteria con posible potencial zoonótico por su relación con la enfermedad de Crohn. Debido a que no hay tratamiento para la PTB, se deben intensificar los mecanismos de control en las producciones.

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Validation of IS900- qPCR assay to assess the presence of Mycobacterium avium subs. paratuberculosis in faecal samples according to the OIE procedure

Russo

Giorgio²,

Leo³

et al. 2022

Preventive Veterinary Medicine

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Field study on bovine paratuberculosis using real-time PCR and liquid culture for testing environmental and individual fecal samples implemented in dairy cow management

Cited by 5 publications

References 32 publications

The Risk of False-Positive Serological Results for Paratuberculosis in Mycobacterium bovis-Infected Cattle

The Risk of False-Positive Serological Results for Paratuberculosis in Mycobacterium bovis-Infected Cattle

Paratuberculosis bovina, revisión de literatura

Validation of IS900- qPCR assay to assess the presence of Mycobacterium avium subs. paratuberculosis in faecal samples according to the OIE procedure

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