Field stimulation-induced responses of circular smooth muscle from guinea-pig stomach

Costall, B.; Naylor, R.J.; Tan, Connie C.W.

doi:10.1007/bf00634264

Cited by 18 publications

(4 citation statements)

References 8 publications

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“…This is in agreement with the responses of longitudinal and circular muscle in the stomach after nerve stimulation. Both muscle layers show cholinergic excitation and non-cholinergic non-adrenergic inhibition after vagal or intramural stimulation [12,36]. In addition it has been shown that circular and longitudinal smooth muscle cells of the guinea pig stomach respond to nerve stimulation with excitatory and inhibitory junction potentials [3,52].…”

Section: Discussionmentioning

confidence: 99%

Projections and neurochemical coding of motor neurones to the circular and longitudinal muscle of the guinea pig gastric corpus

Michel

Reiche

Schemann

2000

Pflugers Arch - Eur J Physiol

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The present study identified projection and neurochemical coding patterns of intrinsic circular (CMN) and longitudinal muscle motor neurones (LMN) in the guinea pig stomach by using the retrograde tracer DiI (1,1'-didodecyl-3,3,3',3'-tetramethylindocarbocya-nine perchlorate) in combination with the immunohistochemical demonstration of choline acetyltransferase (ChAT), enkephalin (ENK), neuropeptide Y (NPY), nitric oxide synthase (NOS) and substance P (SP). Populations of LMN and CMN had similar neurochemical coding and a clear polarity of projection. Taking all DiI-labeled cell bodies as 100%, ascending pathways exhibited the coding ChAT/- (CMN:14.7%/LMN: 18.3%), ChAT/ENK (15.7%/10.1%), ChAT/SP/+/-ENK (19.2%/16.4%), or ChAT/NPY (4.4%/7.6%); descending pathways had the coding NOS/- (13.8%/16.9%), NOS/NPY (9.9%/17%), NOS/ENK (4.4%/1.2%) or NOS/NPY/ENK (13.0%/5.5%). The relative contributions of these populations were not different between CMN and LMN. However, target-specific projection patterns were revealed: most LMN (82%) had longitudinal whereas most CMN (58%) had circumferential projection preferences. The results indicate that gastric circular and longitudinal muscle layers are innervated by ascending excitatory and descending inhibitory pathways in the myenteric plexus. The projection patterns of CMN and LMN were different and followed the orientation of the muscle layers. It is suggested that the specific muscle motor pathways in the gastric myenteric plexus coordinate the reflex-mediated phasic and tonic activity of gastric muscle layers.

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Section: Discussionmentioning

confidence: 99%

Projections and neurochemical coding of motor neurones to the circular and longitudinal muscle of the guinea pig gastric corpus

Michel

Reiche

Schemann

2000

Pflugers Arch - Eur J Physiol

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“…It is not clear how metoclopramide may increase neuronal ACh release in the gut. In guinea-pig isolated ileum or stomach, the action of metoclopramide seems unrelated to its ability to antagonize responses mediated by dopamine (Spedding 1981;Kilbinger et al 1982;Zar et al 1982;Costall et al 1983), but could be related to an effect of metoclopramide on receptors for 5-hydroxytryptamine responsible for modulating ACh release (Kilbinger et al 1982).…”

Section: Diwssionmentioning

confidence: 99%

“…The action of certain substituted benzamide drugs in facilitating gastric emptying may reflect their influence both on central sites and peripherally to enhance stomach muscle contraction. the effect involving an enhancement of cholinergic activity (Costall et al 1983(Costall et al , 1985. Although substituted benzamides are often potent dopamine receptor antagonists, there is no evidence to link this property with a potential to facilitate gastric emptying and to.…”

Section: Diwssionmentioning

confidence: 99%

Effects of metoclopramide and domperidone on cholinergically mediated contractions of human isolated stomach muscle

Sanger

1985

Journal of Pharmacy and Pharmacology

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The experiments examine the actions of metoclopramide and domperidone on the responses evoked by electrical field stimulation or by acetylcholine, in longitudinal muscle strips obtained from human stomach. Electrical field stimulation evoked contractions which were predominantly cholinergically mediated; metoclopramide 0.28-28 microM caused a concentration-dependent increase in the height of these contractions. In the presence of atropine and barium chloride, electrical stimulation evoked relaxations of the stomach muscle, probably by stimulating non-adrenergic, non-cholinergic inhibitory nerves; metoclopramide 28 microM had no effect on these relaxations. Metoclopramide 0.003-2.8 microM had no effect on contractions evoked by exogenous acetylcholine, although higher concentrations of the drug increased the contractions. The results suggest that in human isolated stomach, low concentrations of metoclopramide may increase electrically evoked cholinergic activity by increasing the release of neuronal acetylcholine. Stimulation by metoclopramide of cholinergic activity in the gut may therefore be an important mechanism by which the drug increases gastrointestinal motility during therapy. Cholinergically mediated contractions were not increased by domperidone, and other mechanism(s) of action may therefore be important for this drug.

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“…Many findings suggest that there are dopaminergic nerves and specific dopamine receptors in the gastrointestinal tract and that dopamine and its antagonist can modulate gastrointestinal motility (Costall, Naylor & Tan, 1983; Hernandez et al. , 1984; van Neuten & Schuurkes, 1984; Schuurkes & van Neuten, 1984; Fernandez & Massingham, 1985; Kusunoki, Taniyama & Tanaka, 1985; Orloff, Orloff, Bunnett & Walsh, 1985; Milanes, Martinez & Vargas, 1989).…”

Section: Discussionmentioning

confidence: 99%

Actions of domperidone on the opioid system in isolated guinea‐pig ileum: differences between dopamine antagonists on opioid system

Masuda

Kishioka

Yamamoto

2003

Auton Autocoid Pharmacol

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1. To validate the relationship between the dopaminergic, opioidergic and cholinergic nervous systems, we evaluated the effect of domperidone, a dopamine (D2) antagonist, on the opioid system in myenteric plexus-longitudinal muscle preparation isolated from the guinea-pig ileum. 2. One micromolar of domperidone did not affect the 0.1 Hz-evoked (duration 0.5 ms, maximum intensity) twitch response, but concentration dependently inhibited the twitch response between concentrations of 2 and 20 microM, and the inhibition was maximum after 20-30 min at the highest concentration used (20 microM). 3. Acetylcholine-evoked contraction on basal tension was also not inhibited by 1 microM domperidone, but the contraction was concentration dependently inhibited at concentrations of 10-100 microM in a non-competitive manner. 4. One micromolar of domperidone, however, increased post-tetanic twitch inhibition, an indicator of the release of endogenous opioids. This increase was completely antagonized by 1 microM naloxone. Twitch inhibition induced by dynorphin 1-13 (0.1-10 nM) was not affected by 1 microM domperidone, but increased the maximum twitch inhibition caused by morphine (0.1-1 microM). 6. These results might reflect the existence of an interaction between the dopaminergic and opioidergic system without the inhibition of the cholinergic system. Dopamine antagonists increased opioid action, an action that may depend more on the increased release of endogenous opioids than on supersensitivity of the opioid receptor.

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Field stimulation-induced responses of circular smooth muscle from guinea-pig stomach

Cited by 18 publications

References 8 publications

Projections and neurochemical coding of motor neurones to the circular and longitudinal muscle of the guinea pig gastric corpus

Projections and neurochemical coding of motor neurones to the circular and longitudinal muscle of the guinea pig gastric corpus

Effects of metoclopramide and domperidone on cholinergically mediated contractions of human isolated stomach muscle

Actions of domperidone on the opioid system in isolated guinea‐pig ileum: differences between dopamine antagonists on opioid system

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