Field sensitivity action spectra of cone photoreceptors in the turtle retina

Abstract

The Stiles two‐colour increment threshold technique was applied to turtle cone photoreceptors in order to derive their field sensitivity action spectra. Photoresponses of cone photoreceptors were recorded intracellularly. Flash sensitivities were calculated from small amplitude (< 1 mV) responses. The desensitizing effects of backgrounds of different wavelengths were measured and the background irradiance needed to desensitize the cone by a factor of 10 (1 log unit) was defined as threshold. The reciprocals of these thresholds were used to construct the field sensitivity action spectrum. The field sensitivity action spectra of long‐wavelength‐sensitive (L) and medium‐wavelength‐sensitive (M) cones depended upon the wavelength of the test flash used to measure them. This excludes the possibility that turtle cones can function as single‐colour mechanisms in the Stiles sense. In fourteen L‐cones, the average wavelength of peak sensitivity of the field sensitivity action spectrum was 613.7 ± 7.7 nm for the 500 nm test and 635.6 ± 9.6 nm for the 700 nm test. For six M‐cones, these values were 558.5 ± 6.8 and 628.8 ± 10.6 nm for the 500 and 700 nm tests, respectively. Two physiological mechanisms are suggested as contributing to the dependency of the field sensitivity action spectrum upon test wavelength. One is based upon the transmissivity properties of the coloured oil droplets, while the other hypothesizes excitatory interactions between cones of different spectral type. Computer simulations of the field sensitivity action spectra indicate that both mechanisms are needed in order to account for the dependency of the field sensitivity action spectrum upon the wavelength of the test flash.