Field relevant doses of the fipronil affects gene expression in honey bees Apis mellifera

Astolfi, Aline; Kadri, Samir Moura; Lippi, Isabella Cristina de Castro; Mendes, Daniel Diego; Alonso, Diego Peres; Ribolla, Paulo Eduardo Martins; Orsi, Ricardo de Oliveira

doi:10.1007/s13592-022-00978-7

Cited by 7 publications

(3 citation statements)

References 45 publications

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“…These frames were protected with bags made of tulle fabric and returned to their respective hives. After a period of 24 hours, the protected frames were again removed from the hives, and the newly emerged bees were marked with a non-toxic pen on the pronotum region (Astol et al 2022;Camilli et al 2022). Immediately after marking the bees, the tulle bags were removed, and the frames containing the marked bees were returned to their respective hives.…”

Section: Collection Of Forager Honey Beesmentioning

confidence: 99%

“…For the preparation and sequencing of the RNA-Seq library, honey bees were decapitated, and the compound eyes were removed from each head to prevent potential pigment contamination (Astol et al 2022). Subsequently, the heads were macerated to expose the brain material, which was then used for total RNA extraction.…”

Section: Rna-seq Sequencing and Analysis Of Differentially Expressed ...mentioning

confidence: 99%

“…Gene expression pattern analysis has been employed in various studies, investigating the effects of parasites on bees (Zanni et al 2017), nutritional stress (Corby-Harris et al 2014), mineral supplementation effects on gene expression (Camilli et al 2022), as well as transcriptomic studies evaluating the effect of pesticides on the gene expression pro le of contaminated bees (Li et al 2019; Gao et al 2022; Astol et al 2022). Considering that bees' brains are the main organs affected by neurotoxic pesticides, these pesticides can easily disturb their nervous systems (Li et al 2019).…”

Section: Introductionmentioning

confidence: 99%

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Food contamination with fipronil alters gene expression associated with foraging in Africanized honey bees

Lima,

Lippi,

Scheffer

et al. 2024

Preprint

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Taking into consideration that bees can be contaminated by pesticides through the ingestion of contaminated floral resources, we can utilize genetic techniques to assess effects that are scarcely observed in behavioral studies. This study aimed to investigate the genetic effects of ingesting lethal and sublethal doses of the insecticide fipronil in foraging honey bees during two periods of acute exposure. Bees were exposed to fipronil through contaminated honey syrup at two dosages (LD50 = 0.19 μg/bee; LD50/100 = 0.0019 μg/bee) and for two durations (one and four hours). Following exposure, we measured syrup consumption per bee, analyzed the transcriptome of bee brain tissue, and identified differentially expressed genes (DEGs), categorizing them functionally based on Gene Ontology (GO). The results revealed a significant genetic response in honey bees after exposure to fipronil, regardless of the dosage used. Fipronil affected various metabolic, transport, and cellular regulation pathways, as well as detoxification processes and xenobiotic substance detection. Additionally, downregulation of several DEGs belonging to the Olfactory Binding Protein (OBP) family was observed, suggesting potential physiological alterations in bees that may lead to disoriented behaviors and reduced foraging efficiency.

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Section: Collection Of Forager Honey Beesmentioning

confidence: 99%

Section: Rna-seq Sequencing and Analysis Of Differentially Expressed ...mentioning

confidence: 99%