Field performance of temporary immersion bioreactor-derived sugarcane plants

Lorenzo, José Carlos; Ojeda, Elka; Torregroza-Espinosa, Ana Carolina; Borroto, C.G

doi:10.1007/s11627-001-0133-8

Cited by 40 publications

(22 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several researchers have reported that sugarcane micropropagation is the only realistic means of achieving rapid propagation of new cane varieties, reduction in seed use, and regeneration of large number of true to type plantlets from a small tissue, elimination of pathogens and storage of plant germplasm under aseptic condition (Ali et al, 2004;Lorenzo et al, 2001;Lal and Krishna, 1994). Jalaja et al (2008) reported that within nine (9) months, callus culture of apical meristem produced planting materials from a single spindle which was sufficient to plant one hectare of land.…”

Section: Micropropagation For Rapid Seed Cane Multiplicationmentioning

confidence: 99%

Sugarcane in vitro culture technology: Opportunities for Kenyas sugar industry

Wekesa¹,

Onguso²,

Nyende³

et al. 2015

Afr. J. Biotechnol.

View full text Add to dashboard Cite

Sugarcane (Saccharum officinarum L.) is one of the most important crops in Kenya and has wide range of economic importance. The sugar industry contributes up to 15% to the country's agricultural gross domestic product and an estimated 25% of the population depends on the industry for their livelihood. However, the industry has been facing several challenges including declining yields due to use of poor quality planting materials. There is an increasing pressure to enhance the productivity of sugarcane in order to sustain profitable sugar industries in Kenya, while there are several diseases attacking sugarcane and reducing its quality. Seed multiplication of newly released varieties of sugarcane is one of the major constraints in Kenya as it takes 6-7 years to produce sufficient quantity of improved seed material. In vitro culture offers a practical and fast method for mass propagation of disease-free clonal materials. Successful protocols for shoot tip culture, callus culture, embryo culture, virus free plant production and somatic embryogenesis have already been established. Thus, in vitro technology can be used to enhance productivity of sugarcane in Kenya. Despite several advantages of applying micro-propagation technique in sugarcane such as quick multiplication of newly released varieties, rejuvenation of old deteriorated varieties; production of disease free seed; easy transportation of seed material; elimination of viruses; high cane productivity and sugar yield etc., this technique is not gaining popularity up to the desired extent. There are several constraints like the high cost of production and appearance of some variants in micropropagated population among others. The present article describes the status, challenges and opportunities of in vitro technology for the sugar industry in Kenya. Though, some problems have now been resolved to considerable extents which have been described in this review however, some constraints still require intensive research work to be resolved so that a safe and efficient exploitation of this technique can be ensured in sugarcane seed production programmes for enhanced yields and quality.

show abstract

Section: Micropropagation For Rapid Seed Cane Multiplicationmentioning

confidence: 99%

Sugarcane in vitro culture technology: Opportunities for Kenyas sugar industry

Wekesa¹,

Onguso²,

Nyende³

et al. 2015

Afr. J. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Nowadays, unlike the conventional propagation method, it is the only realistic means of achieving rapid and large scale production of disease free quality planting materials in sugarcane [33][34][35] and an alternative approach for fast multiplication of a variety in its original form [19,36]. It is very effective in entire disease cleansing, rejuvenation and subsequent mass propagation of well adapted and promising varieties facing gradual deterioration in yield, quality and vigor due to accumulation of pathogens during prolonged vegetative cultivation and hence sustains the productive potential of sugarcane crops for a longer period [37,38].…”

Section: Advances In Crop Science and Technologymentioning

confidence: 99%

Effects of 6-Benzyl aminopurine and Kinetin on In Vitro Shoot Multiplication of Sugarcane (Saccharum officinarum L.) Varieties

Tolera¹,

Diro²,

Belew³

2014

Adv Crop Sci Tech

View full text Add to dashboard Cite

In vitro shoot multiplication of two commercial sugarcane varieties grown in Ethiopian sugar estates initiated using shoot tip explants was carried out with the objective to assess the multiplication responses of the sugarcane varieties (B41-227 and N14) under five levels of 6-benzylaminopurine (0, 0.5, 1, 1.5 and 2 mgL -1 ) and kinetin (0, 0.1, 0.25, 0.5, and 1 mgL -1 ) in a completely randomized design with 5x5x2 factorial treatment combinations arrangements. Analysis of variance (ANOVA) showed that the interaction effects of 6-benzylaminopurine, kinetin and the sugarcane genotypes on number of shoots per explant, average shoot length and number of leaves per shoot was very highly significant (P < 0.0001). Murashige and Skoog (MS) medium fortified with 1 mgL -1 6-benzylaminopurine (BAP) and 0.5 mgL -1 kinetin for B41-227; and 2 mgL -1 6-benzylaminopurine (BAP) without kinetin for N14 were found to give optimum shoot proliferation results. On these medium, B41-227 gave 34 ± 1.54 shoots per explant with 6.95 ± 0.01cm average shoot length and 12 ± 0.17 leaves per shoot while N14 produced 21 ± 0.58 shoots per explant with 5.63 ± 0.01 cm average shoot length and 5.4 ± 00 leaves per shoot after 30 days of culture transfer to multiplication medium. Thus, the optimized protocol is useful for rejuvenation, rapid In vitro propagation and production of large quantity quality sugarcane planting materials and hence help minimizes the major limitations hampering sugarcane production in the Ethiopian sugar Estates.

show abstract

“…On the other hand, micropropagation is currently the only realistic means of achieving rapid, large-scale production of disease-free quality planting material as seed canes of newly developed varieties in order to speed up the breeding and commercialization process in Sugarcane (Feldmennet al,1994;Lal & Krishna,1994;Lee , 1987;Lorenzo et al, 2001;Krisnamurthi and Tlaskal, 1974). As a result of which plant regeneration through tissue culture technique would be a viable alternative for improving the quality and productivity in sugarcane.…”

Section: Introductionmentioning

confidence: 99%

Improvement of sugarcane (Saccharum officinarum L. var. Isd. 39) using gamma irradiation and large scale plantlet production from M1 generation through in vitro culture

Akter

Alam

Roy

2016

Jahangirnagar Univ. J. Biol. Sci.

View full text Add to dashboard Cite

The present study was aimed to develop mutant sugarcane (Saccharum officinarum L. var. Isd. 39) using gamma radiation followed by regeneration of those mutants through in vitro technique. Five different doses of gamma radiation (10, 20, 30, 40 and 50 Gy) were applied to the sugarcane setts and among these doses 10 Gy irradiated mutant sugarcane showed the best agronomical traits. In addition, 10 Gy irradiated mutant sugarcane also contained higher sugar concentrations (14.25%) compared to control (14.23%). On the other hand, shoot tip and young folded leaf segments from the 10 Gy irradiated plants (M 1 generation) were used as explants in in vitro culture. It was evident from the experiment that direct shoot regeneration was better than indirect regeneration. Young leaf segments produced the highest percentage (84%) of shoots when cultured on MS+1.0 mg/l Kin + 0.2 mg/l NAA. The mean number of shoots was recorded as 35  1.12 in the same medium. Moreover, addition of 4% sucrose and 10% coconut water (CW) in the same medium increased the number of shoot up to 45 per culture. Best rooting was obtained from shoots cultured on half-strength of MS fortified with 1.0mg/l NAA and 0.5mg/l IBA. Well-rooted plantlets were transferred to poly bag containing soil and compost (2:1) for hardening. During hardening 70% plantlets survived, which were subsequently transferred to the experimental field.

show abstract

Field performance of temporary immersion bioreactor-derived sugarcane plants

Cited by 40 publications

References 9 publications

Sugarcane in vitro culture technology: Opportunities for Kenyas sugar industry

Sugarcane in vitro culture technology: Opportunities for Kenyas sugar industry

Effects of 6-Benzyl aminopurine and Kinetin on In Vitro Shoot Multiplication of Sugarcane (Saccharum officinarum L.) Varieties

Improvement of sugarcane (Saccharum officinarum L. var. Isd. 39) using gamma irradiation and large scale plantlet production from M1 generation through in vitro culture

Contact Info

Product

Resources

About