Field performance of HBsAg rapid diagnostic tests in rural Ethiopia

Orlien, Stian Magnus Staurung; Ahmed, Tekabe Abdosh; Ismael, Nejib Yusuf; Belay, Nega Berhe; Kran, Anne-Marte Bakken; Gundersen, Svein Gunnar; Johannessen, Asgeir

doi:10.1016/j.jviromet.2020.114061

Cited by 3 publications

(2 citation statements)

References 13 publications

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“…A similar study in Burkina Faso also yielded good sensitivities (range of 90.8-92.8%) and excellent specificities (range of 97.3 to ≥99.0%) indicating usefulness of RDT for HBV mass screening in Burkina Faso (124). RDTs carry certain risks for use in blood screening; those tests having lower sensitivity may yield false negative results, particularly in the presence of low HBsAg or HBV/HCV antibody titres, or mutations within the HBsAg antigenic determinant may also prevent accurate detection of HBV infection (102,125).…”

Section: Utility Of Hbv and Hcv Rapid Diagnostic Tests In Lmicmentioning

confidence: 99%

Rapid Diagnostics for Hepatitis B and C Viruses in Low- and Middle-Income Countries

Shenge¹,

Osiowy

2021

Front.Virol.

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The global health challenge posed by hepatitis B virus (HBV) and hepatitis C virus (HCV) persists, especially in low-and-middle-income countries (LMICs), where underdiagnosis of these viral infections remains a barrier to the elimination target of 2030. HBV and HCV infections are responsible for most liver-related mortality worldwide. Infected individuals are often unaware of their condition and as a result, continue to transmit these viruses. Although conventional diagnostic tests exist, in LMIC they are largely inaccessible due to high costs or a lack of trained personnel, resulting in poor linkage to care and increased infections. Timely and accurate diagnosis is needed to achieve elimination of hepatitis B and C by the year 2030 as set out by the World Health Organization Global Health Sector Strategy. In this review rapid diagnostic tests allowing for quick and cost-effective screening and diagnosis of HBV and HCV, are discussed, as are their features, including suitability, reliability, and applicability in LMIC, particularly those within Africa.

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Section: Utility Of Hbv and Hcv Rapid Diagnostic Tests In Lmicmentioning

confidence: 99%

Rapid Diagnostics for Hepatitis B and C Viruses in Low- and Middle-Income Countries

Shenge¹,

Osiowy

2021

Front.Virol.

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“…In conjunction with public vaccination, timely diagnosis of HBV infection is a vital measure to decrease complications and mortality (5). Serological biomarkers such as HBsAg, anti-HBs, and anti-HBc antibodies are useful for diagnosing HBV (6,7).…”

Section: Introductionmentioning

confidence: 99%

Optimization and Application of Quantitative In-House ELISA for Diagnosis of HBsAg and its Correlation with Commercial ELISA and Molecular Kits

Ghorbani,

Daryabour,

Shokri

et al. 2023

Hepat Mon

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Background: Several studies have indicated the role of quantitative hepatitis B surface antigen (HBsAg) evaluation in managing and prognosis of hepatitis B virus (HBV) infection. Thus, quantitative evaluation of HBsAg using cost-effective assays can be an important approach to managing HBV patients. Objectives: This study aimed to set up and apply an in-house quantitative enzyme-linked immunosorbent assay (ELISA) to evaluate HBsAg in research and diagnosis. Methods: New Zealand white rabbits were immunized with HBsAg. Sera were collected 28 days after immunization, and polyclonal HBsAb (antibody to hepatitis B surface antigen) were purified and evaluated. Then, the in-house quantitative ELISA was optimized. Finally, the functional characteristics of the assay were evaluated using 200 plasma samples compared to commercial ELISA and quantitative TaqMan real-time PCR. Results: The assay has a limit of detection (LOD) of 0.5 ng/mL with a specificity and sensitivity of 94% and 97%, respectively. The assay's highest coefficient of variation (CV) values for intra- and inter-assays were 7.23% and 8.59%, respectively. The correlation of the developed assay with commercial ELISA was 0.987 (P-value = 0.024). The correlations of the developed assay and commercial ELISA with quantitative TaqMan real-time PCR were 0.739 and 0.658, respectively (P-value = 0.017). Conclusions: The developed assay has a suitable sensitivity and specificity. It is also reproducible and well-correlated with commercial assays. Most importantly, it is cost-effective and, thus, can be used for detecting and quantifying HBsAg in research and diagnosis.

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Incidence of Hepatitis B Surface Antigen (HBsAg) Infection and Its Relationship with Risk Factors at Janitors at Regional General Hospital dr. M. M. Dunda Limboto Gorontalo

Lalangpuling

Dakio

Nikma

et al. 2023

IJMLST

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Hepatitis B is an inflammatory liver disease caused by the Hepatitis B virus, which can be acute or chronic. The outer membrane protein of Hepatitis B Virus (HBV) is known as Hepatitis B Surface Antigen (HBsAg). Janitors are employees who are tasked with cleaning the hospital environment to keep it clean, because of the the dangers that exist in hospitals, such as disease transmission, can occur if the hospital environment is not kept clean. The purpose of this study was to describe the results and their relationship to risk factors of the Hepatitis B examination on janitors at the Regional General Hospital (RSUD) dr. Mansyoer Mohammad Dunda Limboto Gorontalo. This study used a descriptive method with an accidental sampling technique. The sample in this study amounted to 33 respondents who met the inclusion criteria. Specimen were examined using the rapid test method. The data were presented descriptively and statistically to see the relationship between work and the incidence of Hepatitis B infection. The results obtained were as many as 33 respondents by conducting an HBsAg examination, with the results obtained being 2 reactive people and 31 non-reactive people. The results of statistical tests on the relationship between infection status and age showed a p-value of 0.019 and the relationship between infection status and symptoms showed a p-value of 0.002. The study’s findings revealed that there was a substansial correlation between the respondents’ reported symptoms and their infection status (6% of respondents teste for Hepatitis B).

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Field performance of HBsAg rapid diagnostic tests in rural Ethiopia

Cited by 3 publications

References 13 publications

Rapid Diagnostics for Hepatitis B and C Viruses in Low- and Middle-Income Countries

Rapid Diagnostics for Hepatitis B and C Viruses in Low- and Middle-Income Countries

Optimization and Application of Quantitative In-House ELISA for Diagnosis of HBsAg and its Correlation with Commercial ELISA and Molecular Kits

Incidence of Hepatitis B Surface Antigen (HBsAg) Infection and Its Relationship with Risk Factors at Janitors at Regional General Hospital dr. M. M. Dunda Limboto Gorontalo

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