In this paper, the vibration characteristics of the 1ST-460 vibration subsoiler was analyzed. In order to solve the problem of vibration imbalance caused by the vibration component under the action of multiple groups of vibration shovels, the optimal combination mode of the vibration component under the interaction of multiple groups of shovels was obtained. Piezoelectric accelerometers are fixed symmetrically on the frame of the subsoiler to measure the vibration at different positions. TST5910 dynamic signal test and analysis system is used to collect and process the acceleration data. Non-load tests were carried out with different initial eccentric phase angle combination. Results showed that the combination of [0°, 180°, 180°, 0°] (symmetrically up and down staggered vibration) for the eccentric vibration component of four shovels is the optimal initial phase combination, which can lead to the minimum adverse vibration on the frame. Further tests were conducted under different vibrating frequency and amplitude/eccentric with optimal phase combination. The results showed that, when amplitude/eccentricity ratio was 2.0 and 2.5, frequency of vibration was 6.7 and 8.3 Hz, the required traction force of the subsoiler was stable and significantly reduced, which can improve the soil loosening performance and reduce the harmful vibration on the tractor.