Field of view scanning based quantitative interferometric microscopic cytometers for cellular imaging and analysis

Abstract

Microimaging is of great significance in the biological and medical fields, since it can realize observations acting as important references for cellular research and disease diagnosis. However, traditional microscopy only offers qualitative sample contours; moreover, it is difficult to reach large-amount sample observations limited by the fixed field of view (FoV). To realize massive cellular measurements quantitatively, three designed quantitative interferometric microscopic cytometers based on the FoV scanning are introduced and compared in details in this article. These devices not only retrieve the quantitative sample phase distributions in the extended FoV, but also provide the detailed information of massive cells, such as cellular volume, area, and roundness. Considering their capabilities as quantitative imaging and large-amount sampling, it is believed that these quantitative interferometric microscopic cytometers (QIMCs) can be potentially adopted in high-throughput cell imaging and statistical analysis for both the biological and medical applications.

Measurements on ATP induced cellular fluctuations using real-time dual view transport of intensity phase microscopy

“…However, due to the indispensable time-consuming iteration, ptychography can hardly be used for real-time live cell imaging. Interferometry based techniques such as digital holographic microscopy [28][29][30][31][32] and quantitative interferometric microscopy [33][34][35][36][37][38][39][40] can extract cell phases even from single-shot fringe pattern, indicating its real-time quantitative phase imaging capability. While reference beam and coherent illumination are required, it is difficult to integrate the interferometry with the widely used commercial microscopes.…”