Field measurement of plasma and erythrocyte cholinesterases

Magnotti, Ralph A.; Dowling, Kathryn C.; Eberly, J. Patrick; McConnell, Rob

doi:10.1016/0009-8981(88)90190-8

Cited by 50 publications

(52 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Exposure to OP compounds inhibits mammalian acetylcholinesterase ( AChE ), resulting in the development of symptoms that have their origin at different parts of the nervous system (Coye et al, 1986;Magnotti et al, 1988 ). When cholinesterase is blocked, acetylcholine level increases and builds up in the muscles.…”

Section: Introductionmentioning

confidence: 99%

Acute health effects of organophosphorus pesticides on Tanzanian small-scale coffee growers

Ngowi

Maeda

Partanen

et al. 2001

J Expo Sci Environ Epidemiol

View full text Add to dashboard Cite

Acute health effects of organophosphorus ( OP ) pesticides on coffee farmworkers in 1991 -1992 in Tanzania are reported to provide a basis for concern over farmworkers being overexposed during application. Workers exposed to OP pesticides ( N = 133 ) were drawn from a population of about 240,000 coffee farmers. They were interviewed on symptoms and personal protection, and their erythrocyte acetylcholinesterase ( AChE ) activity was determined during both spraying and nonspraying period. AChE activities during spraying and nonspraying period were comparable ( mean 32.0, SD 7.8 vs. 33.0, SD 8.7 U / g HgB, P= 0.26 ) . The prevalence of cough, headache, abdominal pain, excessive sweating, nausea, excessive salivation, diarrhea, and vomiting did not differ significantly between spraying and nonspraying periods. There was no suggestion of decreased AChE in exposed subjects who complained of OP -related symptoms compared to symptomless exposed subjects. Use of gloves, long boots, head cover, face cover, and coverall was not significantly associated with AChE activity. No marked AChE depression was found during spraying season, which may explain the lack of association between symptoms and AChE. The fact that only moderately toxic OP pesticides were used may indicate that toxicity was not sufficiently high to cause depression. Experience, however, suggests that occupational poisoning remains a potential serious danger in coffee cultivation in Tanzania.

show abstract

Section: Introductionmentioning

confidence: 99%

Acute health effects of organophosphorus pesticides on Tanzanian small-scale coffee growers

Ngowi

Maeda

Partanen

et al. 2001

J Expo Sci Environ Epidemiol

View full text Add to dashboard Cite

show abstract

“…El método electrométrico aplicado por Michel (10) y consolidado por Rider y asociados (12) mide el pH y es la prueba de referencia o estándar para cuantificar la actividad colinesterásica en eritrocitos y plasma (1,2); requiere condiciones de laboratorio y personal bien entrenado. El método espectrofométrico usado por Ellman y aplicado por la técnica de EQM ® es un procedimiento colorimétrico, cinético o de punto final, simple, que no requiere un laboratorista con mucha destreza; es rápido, conveniente y confiable, tiene muy buena precisión y óptima sensibilidad, es más rápido y sensible que Michel y la versión desarrollada en 1978 por la Organización Mundial de la Salud con mini-espectrofotómetro portátil sirve para trabajo de campo y mantiene todas las ventajas enunciadas (13)(14)(15)(16)(17) (36). Estos informes señalan la posibilidad y la conveniencia de disponer de estos modelos para efectuar tales intercambios, lo cual debe hacerse para condiciones específicas de laboratorio, es decir, teniendo en cuenta las condiciones aplicadas al realizar la medición química.…”

Section: Discussionunclassified

“…Las técnicas empleadas están basadas en los principios de: a) la medición del ácido producido de la acetilcolina, acetil betametilcolina o de la butirilcolina; b) la medición de la hidrólisis de la tiocolina (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18).…”

Section: Técnicas De Colinesterasa Eritrocitariaunclassified

“…Específicamente para los plaguicidas inhibidores de la colinesterasa, que continúan siendo responsables de un alto número de intoxicaciones, la determinación de esta enzima se emplea como uno de los mejores indicadores biológicos de efecto en la exposición aguda o crónica (1). Existen varios métodos para la medición de esta enzima (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18). Para la exposición prolongada en bajas dosis se recomienda medir la enzima eritrocitaria, mientras que para la exposición aguda se prefiere la medición de la enzima plasmática (14,15).…”

unclassified

“…Existen varios métodos para la medición de esta enzima (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18). Para la exposición prolongada en bajas dosis se recomienda medir la enzima eritrocitaria, mientras que para la exposición aguda se prefiere la medición de la enzima plasmática (14,15).…”

unclassified

See 2 more Smart Citations

Correlación y conversión entre valores de colinesterasa eritrocitaria medida con las técnicas de Michel y EQM®

Carmona‐Fonseca

2006

biomedica

View full text Add to dashboard Cite

Introducción. Las técnicas para medir colinesterasa eritrocitaria son muchas y es útil tener un modelo matemático que permita interconvertir valores. Objetivo. A partir de datos autóctonos de valores de referencia de actividad colinesterásica medida por las técnicas de Michel y EQM ® hallar una ecuación para transformar unas unidades en otras. Materiales y métodos. Diseño descriptivo, transversal y prospectivo, con sendas muestras representativas de poblaciones laborales adultas, de 18 a 75 años de edad, no expuestas a plaguicidas inhibidores de colinesterasa, vinculadas al Seguro Social y situadas en el valle de Aburrá y en el cercano oriente antioqueño (Antioquia, Colombia). Resultados. En las 827 personas, las pruebas eritrocitarias cuantitativas (Michel y EQM ® ) tienen coeficiente «r» entre 0,666 y coeficiente R 2 de 44%. La correlación es mayor en Aburrá que en Oriente. El modelo lineal en los 827 sujetos es: EQM ® en U/g oxihemoglobina = 9,57509 U/g oxihemoglobina + 29,7914 (Michel en deltas de pH/hora), Michel en deltas de pH/hora = 0,33120 deltas de pH/hora + 0,01487 (EQM ® en U/g oxihemoglobina). Tanto las intersecciones (coeficiente a) como las pendientes (coeficiente b) son significativas en el modelo. En las ecuaciones ajustadas, al excluir doce datos extremos (1,5% de 827), el coeficiente r sube de 0,666 a más de 0,718 y ellas son: EQM U/g oxihemoglobina = 8,18840 U/g oxihemoglobina + 31,3920 (Michel deltas de pH/hora), Michel deltas de pH/hora = 0,292510 deltas de pH/hora + 0,016101 (EQM U/g oxihemoglobina).Conclusión. Este sistema de ecuaciones lineales permitirá convertir unidades Michel (delta PH/hora) en unidades EQM (U/g oxihemoglobina) y viceversa, lo cual facilitará el trabajo en ambientes clínicos y epidemiológicos.Palabras clave: acetilcolina, acetilcolinesterasa, plaguicidas, inhibidores de la colinesterasa, vigilancia epidemiológica. Correlation and interconversion between erythrocyte cholinesterase values obtained by the Michel and the EQM ® techniquesIntroduction. Several techniques are available to measure red cell cholinesterase; therefore, evaluations with several methods provide a measure of concordance.Objective. An equation was formulated to transform native data of reference values to reference units of cholinesterase activity as measured by Michel and EQM ® tests. Materials and methods. The experimental design was descriptive, transversal and prospective. The group sampled was a representative adult working population, aged 18-75, without previous exposure to cholinesterase inhibitors pesticides. The individuals were affiliated to the Social Security System and resided in Valle de Aburrá and Cercano Oriente Antioqueño (Antioquia Province, northwestern Colombia). Results. Of 827 individuals, quantitative erythrocytes (Michel y EQM ® ) tests exhibited "r" coefficients between 0.67 and R 2 coefficient of 44%.,This indicated that one test explained the results in other test in 44% of the cases. The corelation was higher in Aburrá than in Oriente. The linear model for the 827 indiv...

show abstract

Selective activity of butyrylcholinesterase in serum by a chemiluminescent assay

Yavo¹,

Brunetti²,

Fonseca³

et al. 2001

Luminescence

View full text Add to dashboard Cite

In a previous study, we showed that purified commercial esterase activity can be detected in a chemiluminescent assay based on the hydrolysis of 2-methyl-1-propenylbenzoate (MPB) to 2-methyl-1-propenol, which is subsequently oxidized by the horseradish peroxidase (HRP)-H(2)O(2) system. The purpose of this study was to verify the applicability of this assay to human serum. The existence of an esterase activity capable of hydrolysing MPB is indicated by the fact that the MPB-serum-HRP-H(2)O(2) system consumes oxygen and emits light. Both signals were abolished by prior serum heat inactivation and were preserved when serum was stored at < or =4 degrees C. Addition of aliesterase inhibitors, such as fluoride ion and trichlorfon or the cholinesterase inhibitor eserine, totally prevents light emission. The butyrylcholinesterase-specific substrate benzoylcholine causes a delay in both O(2) uptake and light emission, while the specific acetylcholinesterase substrate, acetyl-beta-methylcholine, had practically no effect. Purified butyrylcholinesterase, but not acetylcholinesterase, triggered light emission. The finding that butyrylcholinesterase is responsible for the hydrolysis of MPB in serum should serve as the basis for the development of a specific chemiluminescent assay for this enzyme.

show abstract

Field measurement of plasma and erythrocyte cholinesterases

Cited by 50 publications

References 10 publications

Acute health effects of organophosphorus pesticides on Tanzanian small-scale coffee growers

Acute health effects of organophosphorus pesticides on Tanzanian small-scale coffee growers

Correlación y conversión entre valores de colinesterasa eritrocitaria medida con las técnicas de Michel y EQM®

Selective activity of butyrylcholinesterase in serum by a chemiluminescent assay

Contact Info

Product

Resources

About