Field-Evolved Resistance of the Fall Armyworm (Lepidoptera: Noctuidae) to Synthetic Insecticides in Puerto Rico and Mexico

Gutiérrez-Moreno, Rebeca; Mota-Sánchez, David; Blanco, Carlos A.; Whalon, Mark E.; Terán-Santofimio, Henry; Rodríguez-Maciel, J. C.; DiFonzo, Christina

doi:10.1093/jee/toy372

Cited by 208 publications

(138 citation statements)

References 65 publications

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“…At present, there are no reports of diamide field failure against FAW in Brazil, but this is likely to change, as selection pressure steadily increases, particularly due to the development of Bt toxin resistance, compromising the effectiveness of transgenic crops. Such a scenario is not unlikely, as reported recently from Puerto Rico, where FAW developed high levels of field resistance to chlorantraniliprole and flubendiamide due to increased selection pressure …”

Section: Discussionmentioning

confidence: 77%

“…High levels of FAW resistance to diamides (resistance ratios of 160-and 500-fold against chlorantraniliprole and flubendiamide, respectively) due to frequent applications were recently found in a Puerto Rico population, but the mechanism of diamide resistance was not investigated. 15 Levels of diamide resistance much higher than those reported for FAW from Puerto Rico, were very recently described for a Brazilian field-collected FAW strain, Chlorant-R, resulting from an F2 screen and selection with chlorantraniliprole. 45 The authors reported resistance ratios of > 230-fold and > 42 000-fold against chlorantraniliprole and flubendiamide, respectively.…”

Section: Introductionmentioning

confidence: 97%

“…Irrespective of the presence of Bt resistance, a dramatic increase in insecticide applications (up to 28) in a single cropping season has been described . Such a treatment frequency is conducive to the development of insecticide resistance in FAW, so it is not surprising that high levels of resistance to several chemical classes of insecticides have been reported recently in Puerto Rico . In Brazilian populations of FAW, resistance to a range of synthetic insecticides, addressing different modes of action, including organophosphates, carbamates, pyrethroids, benzoylureas and spinosyns, has been also described …”

Section: Introductionmentioning

confidence: 99%

“…Despite increasing selection pressure, particularly in Brazil, high levels of diamide field resistance in noctuid pests conferred by RyR target‐site mutations, as described for some non‐noctuid lepidopterans, have not been reported. High levels of FAW resistance to diamides (resistance ratios of 160‐ and 500‐fold against chlorantraniliprole and flubendiamide, respectively) due to frequent applications were recently found in a Puerto Rico population, but the mechanism of diamide resistance was not investigated …”

Section: Introductionmentioning

confidence: 99%

“…13 Such a treatment frequency is conducive to the development of insecticide resistance in FAW, so it is not surprising that high levels of resistance to several chemical classes of insecticides have been reported recently in Puerto Rico. 15 In Brazilian populations of FAW, resistance to a range of synthetic insecticides, addressing different modes of action, including organophosphates, carbamates, pyrethroids, benzoylureas and spinosyns, has been also described. [16][17][18][19] The most recent chemical class introduced to the insecticide market in 2007 comprised the phthalic and anthranilic acid diamides, targeting insect ryanodine receptors (RyR).…”

Section: Introductionmentioning

confidence: 99%

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Detection of a ryanodine receptor target‐site mutation in diamide insecticide resistant fall armyworm, Spodoptera frugiperda

Boaventura

Bolzan

Padovez

et al. 2019

Pest Management Science

113

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BACKGROUND Fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith), a major lepidopteran pest in Latin and North America, has very recently invaded the continents of Africa and Asia. FAW has evolved resistance to different insecticides and transgenic corn expressing Bacillus thuringiensis (Bt) toxins. Here, we investigated the extent and mechanisms of resistance to diamide insecticides in a Brazilian field‐collected FAW strain selected using chlorantraniliprole. RESULTS Continuous laboratory selection of a field‐collected FAW strain with chlorantraniliprole resulted in resistance ratios of 225‐fold and > 5400‐fold against chlorantraniliprole and flubendiamide, respectively, when compared with a susceptible strain. Pre‐exposure to different synergists known to inhibit detoxification enzymes did not result in significantly increased larval toxicity, suggesting a minor role for metabolic resistance. Sequencing of the FAW ryanodine receptor (RyR) C‐terminal domains II to VI revealed a single nucleotide polymorphism, resulting in a I4734M mutation recently said to confer target‐site resistance to diamides in lepidopteran pests. Genotyping by pyrosequencing of field‐collected FAW larvae sampled in the 2018 crop season suggests a low resistance allele frequency. Furthermore, we developed a fluorescent polymerase chain reaction (PCR)‐based allelic discrimination assay for rapid genotyping of field‐collected FAW samples, because diamides are increasingly used in Bt−/non‐Bt corn. CONCLUSIONS Recently, the identified RyR mutation has been shown to confer field resistance in other lepidopteran pests such as diamondback moth, tomato leafminer and striped rice stem borer. The developed PCR‐based allelic discrimination assay will help to monitor the frequency and future spread of diamide resistance allele in FAW field populations and help to implement appropriate resistance management measures. © 2019 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 77%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Detection of a ryanodine receptor target‐site mutation in diamide insecticide resistant fall armyworm, Spodoptera frugiperda

Boaventura

Bolzan

Padovez

et al. 2019

Pest Management Science

113

View full text Add to dashboard Cite

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Fall‐armyworm invasion, control practices and resistance breeding in Sub‐Saharan Africa

Matova

Kamutando

Magorokosho³

et al. 2020

Crop Science

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Fall armyworm [Spodoptera frugiperda (J.E. Smith); FAW] invasion has exacerbated maize (Zea mays L.) crop yield losses in sub‐Saharan Africa (SSA), already threatened by other stresses, especially those that are climate‐change induced. The FAW is difficult to control, manage, or eradicate, because it is polyphagous and trans‐boundary, multiplies fast, has a short life cycle and migrates easily, and lacks the diapause growth phase. In this study, FAW and its impact in Africa was reviewed, as well as past and present control strategies for this pest. Pesticides, cultural practices, natural enemies, host‐plant resistance, integrated pest management (IPM), and plant breeding approaches were examined as possible control strategies. It was concluded that an IPM control strategy, guided by cultural approaches already being used by farmers, and what can be adopted from the Americas, coupled with an insect‐resistance management strategy, is the best option to manage this pest in Africa. These strategies will be strengthened by breeding for multi‐trait host‐plant resistance through stacking of genes for different modes of control of the pest.

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Resistance of Spodoptera frugiperda (Lepidoptera: Noctuidae) to spinetoram: inheritance and cross‐resistance to spinosad

Lira

Bolzan

Nascimento

et al. 2020

Pest Management Science

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BACKGROUNDThe use of spinosyn insecticides is one of the major control strategies of the fall armyworm, Spodoptera frugiperda (J. E. Smith) in Brazil. In this study, we selected a spinetoram‐resistant strain from a field‐population of S. frugiperda to characterize the inheritance of the resistance and cross‐resistance relationship between spinosyn insecticides.RESULTSThe values of LC50 (95% CI) obtained from concentration–response bioassays were 0.63 (0.55–0.73) μg spinetoram mL−1 for the susceptible strain (SUS), and 1170.96 (1041.61–1323.89) μg spinetoram mL−1 for the strain resistant to spinetoram (SPT‐R). These values resulted in a resistance ratio of 1844‐fold. The SPT‐R strain showed cross‐resistance with spinosad (resistance ratio = 1196‐fold). The reciprocal crosses showed LC50 values of 3.91 (2.97–5.84) and 5.37 (4.52–6.52) μg spinetoram mL−1, suggesting that the resistance of S. frugiperda to spinetoram is autosomal and incompletely recessive. The backcrosses of the F1 progeny with the SPT‐R strain suggest a resistance with polygenic effect. Estimates of the effective number of loci with equal contributions to the resistance effect were from 1.18 to 1.76, suggesting that resistance to spinetoram is associated with a few genes.CONCLUSIONThe inheritance pattern of resistance of S. frugiperda to spinetoram was characterized as autosomal, incompletely recessive and polygenic. Cross‐resistance between spinosyns was confirmed in S. frugiperda. The importance of this information for implementing insect resistance management strategies is discussed in this paper. © 2020 Society of Chemical Industry

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Field-Evolved Resistance of the Fall Armyworm (Lepidoptera: Noctuidae) to Synthetic Insecticides in Puerto Rico and Mexico

Cited by 208 publications

References 65 publications

Detection of a ryanodine receptor target‐site mutation in diamide insecticide resistant fall armyworm, Spodoptera frugiperda

Detection of a ryanodine receptor target‐site mutation in diamide insecticide resistant fall armyworm, Spodoptera frugiperda

Fall‐armyworm invasion, control practices and resistance breeding in Sub‐Saharan Africa

Resistance of Spodoptera frugiperda (Lepidoptera: Noctuidae) to spinetoram: inheritance and cross‐resistance to spinosad

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