Field Evaluation of Transgenic Squash Containing Single or Multiple Virus Coat Protein Gene Constructs for Resistance to Cucumber Mosaic Virus, Watermelon Mosaic Virus 2 and Zucchini Yellow Mosaic Virus

Tricoli, David M.; Carney, K.; Russell, Paul F.; McMaster, J. Russell; Groff, David; Hadden, K. C.; Himmel, P. T.; Hubbard, J. P.; Boeshore, Maury L.; Quemada, Hector

doi:10.1038/nbt1295-1458

Cited by 194 publications

(136 citation statements)

References 24 publications

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“…Subsequently, genes other than those encoding CPs were shown to confer effective protection against infection. These important findings opened up a practical approach with vast implications for disease control that is being applied rapidly to produce disease-resistant cultivars of various crop plants (e.g., see Tricoli et al, 1995). However, despite the rapid commercial adaptation of !he strategy, an understanding of the mechanisms through which interference with the infection process is mediated by viral transgenes has developed more slowly (Scholthof et al, 1993;Lomonossoff, 1995).…”

Section: Pathogen-derived Resistance and The Control Of Virus Diseasementioning

confidence: 99%

Plant-Microbe Interactions: Life and Death at the Interface.

1996

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Section: Pathogen-derived Resistance and The Control Of Virus Diseasementioning

confidence: 99%

Plant-Microbe Interactions: Life and Death at the Interface.

1996

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“…This method has been used to develop resistance to cucurbit potyviruses in different cucurbit species (Tricoli et al, 1995;Fuchs et al, 1998;Compton et al, 2004). However, a breeding program associated to biotechnological tools depends upon the development of an efficient in vitro plant regeneration system.…”

Section: Introductionmentioning

confidence: 99%

In vitro organogenesis in watermelon cotyledons

Krug

Stipp

Martinelli

et al. 2005

Pesq. agropec. bras.

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-The objective of this work was to study the in vitro organogenesis of Citrullus lanatus, by the induction of adventitious buds in cotyledon segments cultured in medium supplemented with cytokinin. Explants were collected from one, three and five-day-old in vitro germinated seedlings, considering the distal and proximal cotyledon regions. The data obtained showed that in vitro organogenesis of watermelon occurred with higher efficiency, when cotyledon segments from the proximal region collected from three-day-old seedlings were cultivated in medium MS, supplemented with BAP (1 mg L -l ) and coconut water (10%). The histological study showed that the organogenesis occurs directly, without callus formation, on epidermal and subepidermal layers of the explants. Adventitious shoots were characterized by the development of shoot apical meristem and leaf primordia. The formation of protuberances, that do not develop into adventitious buds, was also observed.Index terms: Citrullus lanatus, histological study, adventitious bud. Organogênese in vitro em cotilédones de melanciaResumo -O objetivo do trabalho foi estudar a organogênese in vitro de C. lanatus, pela indução de gemas adventícias, em segmentos de cotilédones, cultivados em meio de cultura suplementado com citocinina. Os explantes consistiram de segmentos das regiões distal e proximal de cotilédones, coletados de plantas germinadas in vitro com um, três e cinco dias de idade. Os dados obtidos mostram que a organogênese de melancia, in vitro, ocorre com maior eficiência em segmentos da região proximal dos cotilédones, coletados de plântulas com três dias de idade e cultivados em meio de cultura MS, suplementado com a combinação BAP (1 mg L -l ) e água de coco (10%). Pelo estudo histológico, verificou-se que a organogênese ocorre diretamente, sem a formação de calo, na epiderme e subepiderme do explante. As gemas adventícias foram caracterizadas pela presença de meristema apical e primórdios foliares. Observou-se, também, o desenvolvimento de protuberâncias que não se desenvolvem em gemas adventícias.Termos de indexação: Citrullus lanatus, estudo histológico, gema adventícia.

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“…ovifera) lines and derivatives of these lines have been deregulated and commercialized in the United States. Transgenic line ZW-20 which expresses the coat protein (CP) genes of Zucchini yellow mosaic virus (ZYMV) and Watermelon mosaic virus (WMV), and is resistant to these two viruses (Fuchs and Gonsalves, 1995;Tricoli et al, 1995) received exemption status in 1994 (Medley, 1994). Transgenic line CZW-3 which expresses the CP genes of Cucumber mosaic virus (CMV), ZYMV, and WMV, and is resistant to these three viruses (Fuchs et al, 1998;Tricoli et al, 1995) received exemption status in 1996 (Acord, 1996).…”

Section: Introductionmentioning

confidence: 99%

Movement of coat protein genes from a commercial virus-resistant transgenic squash into a wild relative

Fuchs

Chirco

Gonsalves

2004

Environ. Biosafety Res.

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We monitored pollen-mediated transgene dissemination from commercial transgenic squash CZW-3 into its wild relative Cucurbita pepo ssp. ovifera var. texana (C. texana). Transgenic squash CZW-3 expresses the neomycin phosphotransferase II (nptII) gene and the coat protein (CP) genes of Cucumber mosaic virus (CMV), Zucchini yellow mosaic virus (ZYMV), and Watermelon mosaic virus (WMV); thereby, it is resistant to these three aphid-borne viruses. The rate of NPT II and CP transgene introgression increased with overlapping flowering patterns and a high ratio of transgenic F1 hybrids (C. texana × CZW-3) to C. texana. Transgene transfer also readily occurred from transgenic F1 hybrids into C. texana over three generations in field settings where test plants grew sympatrically and viruses were not severely limiting the growth, and fruit and seed production of C. texana. In contrast, introgression of the transgenes into C. texana was not sustained under conditions of high viral disease pressure. As expected, C. texana progeny that acquired the CP transgenes exhibited resistance to CMV, ZYMV, and WMV. This is the first report on transgene dissemination from a transgenic crop that exhibits disease resistance and hybridizes with a wild plant species without loss of fertility.

show abstract

Field Evaluation of Transgenic Squash Containing Single or Multiple Virus Coat Protein Gene Constructs for Resistance to Cucumber Mosaic Virus, Watermelon Mosaic Virus 2 and Zucchini Yellow Mosaic Virus

Cited by 194 publications

References 24 publications

Plant-Microbe Interactions: Life and Death at the Interface.

Plant-Microbe Interactions: Life and Death at the Interface.

In vitro organogenesis in watermelon cotyledons

Movement of coat protein genes from a commercial virus-resistant transgenic squash into a wild relative

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