Field experiments were conducted at two different geographical regions viz., tropical region (Naraseepuram village, Coimbatore) and temperate region (Kookal village, Kotagiri, The Nilgiris) in summer and winter season’s to assess the bioefficacy of insecticides used against Diamondbackmoth (DBM), Plutella xylostella and their potential impact on natural enemies in cauliflower. The results revealed that the diamide insecticides like cyantraniliprole 10.26 OD @ 60 g a.i ha-1, chlorantraniliprole 18.50 SC @ 10 g a.i ha-1 and flubendiamide 20 WG @ 18.24 g a.i ha-1 registered more than 95 percent population reduction over untreated control followed by emamectin benzoate 5 SG @ 10 g a.i ha-1 and thiodicarb 75 WP @ 750 g a.i ha-1. Quinalphos 25 EC @ 500 g a.i ha-1and chlorpyriphos 20 EC @ 400 g a.i ha-1 were observed as least effective among the treated insecticides. The diamide insecticides recorded considerably less toxic effect on the natural enemies, especially spiders and coccinellids. Population reduction of natural enemies was noticed immediately after insecticide spray but it gradually increased and was recorded on par with the control population. Hence, cyantraniliprole 10.26 OD @ 60 g a.i ha-1, chlorantraniliprole 18.50 SC @ 10 g a.i ha-1 and flubendiamide 20 WG @ 18.24 g a.i ha-1 can be used as potential component in the integrated pest management against DBM in cole crops.