Field evaluation of the response ofAedes albopictus(Stegomyia albopicta) to three oviposition attractants and different ovitrap placements using black and clear autocidal ovitraps in a rural area of Same,Timor‐Leste

Abstract

Known oviposition attractants or stimulants were compared, singly and in combination, using inexpensive autocidal ovitraps designed to trap emerging adults, in a rural area of Timor-Leste during the dry season. In this area, the dengue vector Aedes albopictus (Stegomyia albopicta) Skuse (Diptera: Culicidae) was abundant, but Aedes aegypti (Stegomyia aegypti) L. was not detected. The attractants were: (a) a compound found in Aedes eggs (dodecanoic acid); (b) components of nitrogen, phosphorous and potassium-based (NPK) fertilizer, and (c) infusions of discarded cigarette butts. A solution of ammonium phosphate and potassium nitrate was significantly more attractive to gravid Ae. albopictus than water only. Dodecanoic acid and cigarette butt infusions were not significantly more attractive than the control; however, they attracted various other Diptera and many non-culicid larvae developed in ovitraps in which these substances were used; thus, the presence of eggs or larvae of other species may have deterred Aedes oviposition. Significantly more Aedes eggs were found in ovitraps under vegetation than in ovitraps placed inside houses or against external walls. Clear-sided ovitraps in which black mesh was placed over a black ring floating on the water surface collected significantly fewer eggs than black ovitraps with identically placed mesh and rings.

From tissue engineering to mosquitoes: biopolymers as tools for developing a novel biomimetic approach to pest management/vector control

“…On the other hand, ovitraps targeting gravid females (e.g., the traditional ovitrap), one of the most widely employed and inexpensive passive surveillance/monitoring tools for detecting the presence of A. albopictus , already use an approach based on the characteristics of Aedes oviposition behaviour. They exploit the propensity of container-breeding mosquitoes to lay their eggs in small [ 71 ] artificial water collections, providing an oviposition site similar to those available in urban contexts [ 78 ]. These artificial oviposition sites have been converted from monitoring to a lethal ovitrap (LOT) [ 79 ] by adding an insecticidal ingredient (chemical or biological) [ 80 ], obtaining a low-cost device proven (under certain conditions) to reduce mosquito population density in large-scale campaigns [ 48 ].…”

“…Entomopathogenic fungi such as Bb or Metarhizium anisopliae are interesting (but not exhaustive) examples of potentially embeddable biopesticides. The feasibility of embedding these insect pathogens has already been proven [77][78][79], particularly at the conidial stage. Usually, conidia are suspended in biopolymeric solutions (e.g., alginate), able to form micro shells or spheres containing the pathogens and to preserve their vitality and persistence when applied (usually by spraying after rehydration) [28,109].…”

The Potential Test of the Mosquito Oviposition Preference Using Similar Subtracts: Colonized Water and Aides Larvae Extract

“…One of the environmentally friendly and costeffective control efforts is the use of mosquito attractants (attractants) that are inserted into a trap called an ovitrap. The attractant materials that have been widely researched/used so far include chemicals [9], semiochemicals [10], [11], [12], as well as organic substances in the form of flowers and fruits [13] as well as water hyacinth infusion [14]. However, not many have used attractants from similar basic materials (similar substrates) in the form of colonized water and larvae extract.…”

Dengue Virus Infections among Peace Corps Volunteers in Timor-Leste, 2018–2019

“…Dengue is endemic in Timor-Leste where mosquito vectors Aedes aegypti and Aedes albopictus are present, and multiple epidemics have occurred over the past two decades. 7 , 8 Because of poor access to and use of health care, high case-fatality rates (i.e., 12%) have been reported during prior epidemics. 9 , 10 During January–April 2019, the World Health Organization (WHO) office in Timor-Leste reported an increase in the number of cases of dengue ( n = 532) compared with the same period in the previous year ( n = 317) 11 and 5 deaths, consistent with a dengue epidemic.…”