Field Evaluation of the Relationship Between Chlorophyll Content in Basil Leaves and a Portable Chlorophyll Meter (Spad-502) Readings

Marandu Palisadegrass Mineral Nutrition and Production Related to Nitrogen and Potassium Supply

“…Lavres and Monteiro (2010) reported a similar correlation between SPAD readings and dry mass yield of Mombaça guineagrass. A significant relationship between SPAD readings and leaf chlorophyll (total, a, and b) in basil (Ocimum basilicum) was found by Ruiz-Espinoza et al (2010). Manarin and Monteiro (2002) reported an N concentration of 16.0 g kg −1 and a SPAD reading of 45 in the first harvest of Mombaça guineagrass, and 16.5 g kg −1 and a SPAD reading of 41 in the second.…”

“…The SPAD readings taken in 'Marandu' palisadegrass were related directly to N concentrations in the tissue (Figure 3), as has been reported (Wood et al, 1992;Lavres and Monteiro, 2006;Batista and Monteiro, 2007;Ruiz-Espinoza et al, 2010;Batalha Neto et al, 2011). Chapman and Barreto (1997) reported that 50 to 70% of N in leaves is in protein and enzymes associated with chloroplasts, and therefore the N concentration are correlated directly with the green intensity of leaves.…”

“…Assays for nitrate reductase activity (At-RNA) and chlorophyll meter readings (SPAD), which measures chlorophyll concentrations, have been used to assess plant N status for increasing forage production (Johnson et al, 1976), but SPAD measurements of chlorophyll concentrations are more convenient because they are nondestructive (Ruiz-Espinoza et al, 2010) and can be related to dry matter production, At-RNA, and plant N content (Lavres and Monteiro, 2006;Lavres et al, 2010;Yildirim et al, 2011).…”

Chlorophyll Content of Aphid-Infested Seedling Leaves of Fifteen Maize Genotypes

“…Portable leaf-clip-type chlorophyll meters perform rapid, repetitive and nondestructive estimates of Chl concentrations in living plants (Hawkins et al, 2009;Jinwen et al, 2009;Coste et al, 2010;Gholizadeh et al, 2011;Shrestha et al, 2012;Ghosh et al, 2013). The use of these reliable and advanced instruments for measurement of green pigment content is not restricted to controlled laboratory bioassays but can be extended to experiments in the plants' natural environment (Uddling et al, 2007;Ruiz-Espinoza et al, 2010;Neto et al, 2011;Cerovic et al, 2012). SPAD-502 chlorophyll meters have been used to obtain accurate measurements in arbitrary SPAD units reflecting absolute foliar Chl concentrations (Arunyanark et al, 2008;Marenco et al, 2009;Mielke et al, 2010;Ling et al, 2011;Shrestha et al, 2012).…”

Spad Greenness to Estimate Genotypic Variation in Flag Leaf Chlorophyll in Spring Wheat Under Mediterranean Conditions

“…SPAD readings have been used to predict chlorophyll content in a large number of crop species, such as corn (Markwell et al, 1995), sorghum and pigeonpea (Yamamoto et al, 2002), soybean (Fritschi and Ray, 2007), wheat and potato (Uddling et al, 2007) and basil (Ruiz-Espinoza et al, 2010). A number of studies, restricted to one or a small number of genotypes, have reported a curvilinear relationship between extractable chlorophyll and SPAD values in wheat (Monje and Bugbee, 1992;Castelli et al, 1996;Richardson et al, 2002;Uddling et al, 2007).…”