Field evaluation of the establishment potential of wmelpop Wolbachia in Australia and Vietnam for dengue control

Nguyen, Tran; Nguyen, Hoa L; Nguyen, Thu Yen; Vu, Sinh Nam; Tran, Nhu Duong; Le, Thanh Tuan; Vien, Quang Mai; Bui, T. C.; Le, Huu Tho; Kutcher, Stan; Hurst, Tim; Duong, Thi Thu Huong; Jeffery, Jason A. L.; Darbro, Jonathan M.; Kay, Brian H.; Iturbe‐Ormaetxe, Iñaki; Popovici, Jean; Montgomery, Brian L.; Turley, Andrew P.; Zigterman, Flora; Cook, Helen; Cook, Peter E.; Johnson, Petrina H.; Ryan, Peter; Paton, Chris; Ritchie, Scott A.; Simmons, Cameron P.; O’Neill, Scott L.; Hoffmann, Ary A.

doi:10.1186/s13071-015-1174-x

Cited by 194 publications

(196 citation statements)

References 36 publications

(77 reference statements)

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“…A. aegypti carrying these infections are strongly refractory to dengue [2, 33], but we know very little about the relative fitness of infected mosquitoes developing in the field and subject to food limitation (but see Ross et al [34]). Even in the laboratory the w MelPop strain imposes high fitness costs on A. aegypti [3], and field releases have failed to establish w MelPop in wild populations [35]. …”

Section: Discussionmentioning

confidence: 99%

Predicting Wolbachia invasion dynamics in Aedes aegypti populations using models of density-dependent demographic traits

et al. 2016

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BackgroundArbovirus transmission by the mosquito Aedes aegypti can be reduced by the introduction and establishment of the endosymbiotic bacteria Wolbachia in wild populations of the vector. Wolbachia spreads by increasing the fitness of its hosts relative to uninfected mosquitoes. However, mosquito fitness is also strongly affected by population size through density-dependent competition for limited food resources. We do not understand how this natural variation in fitness affects symbiont spread, which limits our ability to design successful control strategies.ResultsWe develop a mathematical model to predict A. aegypti–Wolbachia dynamics that incorporates larval density-dependent variation in important fitness components of infected and uninfected mosquitoes. Our model explains detailed features of the mosquito–Wolbachia dynamics observed in two independent experimental A. aegypti populations, allowing the combined effects on dynamics of multiple density-dependent fitness components to be characterized. We apply our model to investigate Wolbachia field release dynamics, and show how invasion outcomes can depend strongly on the severity of density-dependent competition at the release site. Specifically, the ratio of released relative to wild mosquitoes required to attain a target infection frequency (at the end of a release program) can vary by nearly an order of magnitude. The time taken for Wolbachia to become established following releases can differ by over 2 years. These effects depend on the relative fitness of field and insectary-reared mosquitoes.ConclusionsModels of Wolbachia invasion incorporating density-dependent demographic variation in the host population explain observed dynamics in experimental A. aegypti populations. These models predict strong effects of density-dependence on Wolbachia dynamics in field populations, and can assist in the effective use of Wolbachia to control the transmission of arboviruses such as dengue, chikungunya and zika.Electronic supplementary materialThe online version of this article (doi:10.1186/s12915-016-0319-5) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Predicting Wolbachia invasion dynamics in Aedes aegypti populations using models of density-dependent demographic traits

et al. 2016

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“…19 The scale-up potential, trade-off between the fitness cost of these variants and their virus-blocking ability, and impact of introducing modified organisms into the ecosystem need to be further evaluated. 20 Finally, community-based approaches that include entomological surveillance and evidence-based, active participation of affected populations in source reduction have been shown to result in reduced household Aedes entomological indices, DENV infection incidence, and dengue disease. 15,21 For vector control efforts to work, they need to involve community participation and be sustained year-to-year, during inter-epidemic periods, and especially in high-risk locations.…”

Section: Vector Controlmentioning

confidence: 99%

Dengue: knowledge gaps, unmet needs, and research priorities

Katzelnick

Coloma

Harris

2017

The Lancet Infectious Diseases

160

162

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Summary Dengue virus (DENV) is a mosquito-borne pathogen that causes up to ~100 million dengue cases each year, placing a major public health, social and economic burden on numerous low- and middle-income countries (LMICs). Major advances by scientists, vaccine developers, and affected communities are revealing new insights and enabling novel interventions and approaches to dengue prevention and control. Such research has highlighted further questions about both the basic understanding of dengue and efforts to develop new tools. We discuss existing approaches to dengue diagnostics, disease prognosis, surveillance, and vector control in LMICs as well as potential consequences of vaccine introduction. We also summarize current knowledge and recent insights into dengue epidemiology, immunology, and pathogenesis, and their implications for understanding natural infection and current and future vaccines.

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“…Therefore, biocontrol strategies are desirable, and the use of the endosymbiotic bacterium Wolbachia has been proposed as a possibility [9–12]. Also, control strategies based on naturally occurring microbial pathogens emerged as another promising alternative to control insects.…”

Section: Introductionmentioning

confidence: 99%

Isolation of fungi from dead arthropods and identification of a new mosquito natural pathogen

et al. 2016

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BackgroundInsects are well known vectors of human and animal pathogens and millions of people are killed by mosquito-borne diseases every year. The use of insecticides to target insect vectors has been hampered by the issues of toxicity to the environment and by the selection of resistant insects. Therefore, biocontrol strategies based on naturally occurring microbial pathogens emerged as a promising control alternative. The entomopathogenic fungus Beauveria bassiana is well characterized and have been approved by the United States Environmental Protection Agency as a pest biological control method. However, thousands of other fungi are unexploited and it is important to identify and use different fungi for biocontrol with possibly some vector specific strains. The aim of this study was to identify new fungal entomopathogens that may be used as potential mosquito biocontrol agents.MethodsCadavers of arthropods were collected from pesticide free areas and the fungi associated isolated, cultured and identified. Then the ability of each isolate to kill laboratory insects was assayed and compared to that of B. bassiana.ResultsIn total we have isolated and identified 42 fungal strains from 17 different arthropod cadavers. Twenty four fungal isolates were cultivated in the laboratory and were able to induce sporulation. When fungal spores were microinjected into Drosophila melanogaster, eight isolates proved to be highly pathogenic while the remaining strains showed moderate or no pathogenicity. Then a selection of isolates was tested against Aedes mosquitoes in a model mimicking natural infections. Only one fungus (Aspergillus nomius) was as pathogenic as B. bassiana and able to kill 100 % of the mosquitoes.ConclusionThe obtained results are encouraging and demonstrate the feasibility of this simple approach for the identification of new potential mosquito killers. Indeed, it is essential to anticipate and prepare biocontrol methods to fight the expansion of mosquitoes’ habitat predicted in certain geographical areas in association with the occurring climatic changes.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-016-1763-3) contains supplementary material, which is available to authorized users.

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Field evaluation of the establishment potential of wmelpop Wolbachia in Australia and Vietnam for dengue control

Cited by 194 publications

References 36 publications

Predicting Wolbachia invasion dynamics in Aedes aegypti populations using models of density-dependent demographic traits

Predicting Wolbachia invasion dynamics in Aedes aegypti populations using models of density-dependent demographic traits

Dengue: knowledge gaps, unmet needs, and research priorities

Isolation of fungi from dead arthropods and identification of a new mosquito natural pathogen

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