Field evaluation of lentil cultivars inoculated with Rhizobium leguminosarum bv. viciae strains for nitrogen fixation using nitrogen-15 isotope dilution

Hafeez, Fauzia Yusuf; Shah, Neal; Malik, K. A.

doi:10.1007/s003740050625

Cited by 31 publications

(45 citation statements)

References 15 publications

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“…Ndfa values of 83-98% for cowpea are in line with findings of Naab on symbiotic effectiveness, nodulation, N 2 fixation and biomass yield has been reported in other studies (Hafeez et al 2000;Thrall et al 2011). The variability observed in nodulation, shoot dry weight and BNF for both legume species in the 10 different soils can be attributed to the differences in competitive ability and effectiveness of the indigenous rhizobial populations in these soils.…”

Section: Discussionsupporting

confidence: 89%

Potential of indigenous bradyrhizobia versus commercial inoculants to improve cowpea (Vigna unguiculataL. walp.) and green gram (Vigna radiataL. wilczek.) yields in Kenya

Mathu

Herrmann

Pypers

et al. 2012

Soil Science and Plant Nutrition

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Limited information is available on reduced cowpea (Vigna unguiculata L. Walp.) and green gram (Vigna radiata L.Wilczek.) yields in Kenya. Declining soil fertility and absence or presence of ineffective indigenous rhizobia in soils are assumptions that have been formulated but still require to be demonstrated. In this study, soils were collected from legume growing areas of Western (Bungoma), Nyanza (Bondo), Eastern (Isiolo), Central (Meru) and Coast (Kilifi) provinces in Kenya to assess indigenous rhizobia in soils nodulating cowpea and green gram under greenhouse conditions. Our results showed that highest nodule fresh weights of 4.63 and 3.32 g plant À1 for cowpea and green gram were observed in one soil from Isiolo and another from Kilifi, respectively, suggesting the presence of significant infective indigenous strains in both soils. On the other hand, the lowest nodule fresh weights of 2.17 and 0.72 g plant À1 were observed in one soil from Bungoma for cowpea and green gram, respectively. Symbiotic nitrogen (N) fixation by cowpea and green gram was highest in Kilifi soil with values of 98% and 97%, respectively. A second greenhouse experiment was undertaken to evaluate the performance of commercial rhizobial inoculants with both legumes in Chonyi soil (also from Coast province) containing significant indigenous rhizobia [>13.5 Â 10 3 Colony Forming Units (CFU) g À1]. Rhizobial inoculation did not significantly (P < 0.05) affect nodulation, biomass yield and shoot N content in cowpea and green gram compared with controls. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the 16S-23S rDNA intergenic spacer (IGS) region analysis of nodules revealed six groups of which only IGS Group IV corresponded with those from commercial inoculants applied, indicating a lower competitiveness of inoculated strains. In cowpea, IGS III was dominant in nodules of plants inoculated with Biofix and Rizoliq commercial inoculants, and the uninoculated control treatment (63.2, 60 and 52.9%, respectively). Similarly, in green gram, IGS Group III was dominant in nodules of plants inoculated with Biofix 704 and Rizoliq commercial inoculants, and the uninoculated control treatment (75, 73.7 and 61.1%, respectively). Our results suggest that the systematic inoculation of both legumes with current available commercial inoculants to improve biomass yields is not necessary in these regions of Kenya. Also, according to our study, it would make sense to promote the utilization of indigenous strains performing well with both legumes.

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Section: Discussionsupporting

confidence: 89%

Potential of indigenous bradyrhizobia versus commercial inoculants to improve cowpea (Vigna unguiculataL. walp.) and green gram (Vigna radiataL. wilczek.) yields in Kenya

Mathu

Herrmann

Pypers

et al. 2012

Soil Science and Plant Nutrition

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“…However, the low levels of starter fertiliser N (<50 kg/ha), together with fixed N improves nodulation, N-fixation and grain yield in various legume crops (Herridge et al . 1995;Hafeez et al . 2000;Shah et al 2000).…”

Section: Resultsmentioning

confidence: 94%

Residual effect of nitrogen fixed by mungbean (Vigna radiata) and blackgram (Vigna mungo) on subsequent rice and wheat crops

Ahmad¹,

Hafeez²,

Mahmood³

et al. 2001

Aust. J. Exp. Agric.

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Annual crop legumes, grown in rotation with cereal crops, contribute to the total pool of nitrogen in the soil and improve the yield of cereals. The present study aimed at the quantification of nitrogen fixation by mungbean and blackgram using 15 N isotopic dilution methodology; and the quantification of grain and nitrogen yield differences of succeeding rice and wheat crops compared with a cereal-cereal rotation. There were 2 experiments in separate fields but with the same layout: in experiment 1, rice followed the mungbean and blackgram varieties and in experiment 2, wheat followed the mungbean and blackgram varieties. Nitrogen fixed ranged from 26 to 36 kg/ha in experiment 1 and from 30 to 36 kg/ha in experiment 2. Soil nitrogen spared by legume crops ranged from 2 to 26 kg/ha in experiment 1 and from 4 to 23 kg/ha in experiment 2. Rice paddy yields were 0.6-1.1 t/ha higher in the legume-cereal rotation than in the cereal-cereal sequence. Similarly, wheat grain yields were 0.5-1.1 t/ha higher in the legume-cereal rotation.

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“…In Pakistan, soils have either nil or very low viable count of effective rhizobia and low nitrogen-supplying capacity because of low organic matter content (0.3-1.0%) (Achakzai, 2007). Therefore, there is need to inoculate legumes that had not been previously grown in a particular area as previously done in many parts of the world (Hafeez et al, 2000(Hafeez et al, , 2001O' Hara, 2001;Bruno et al, 2003;Naeem et al, 2004). Phosphorus (P) and nitrogen (N) play specific role in symbiotic N 2 -fixation through their effects on nodulation and N 2 -fixation process (O' Hara et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Effect of Rhizobium inoculation and NP fertilization on growth, yield and nodulation of soybean (Glycine max L.) in the sub-humid hilly region of Rawalakot Azad Jammu and Kashmir, Pakistan

Tahir¹,

Abbasi²,

Rahim³

et al. 2009

Afr. J. Biotechnol.

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Effect of Rhizobium inoculation and NP fertilization on growth, yield and nodulation of soybean (Glycine max L.) in the sub-humid hilly region of Rawalakot Azad This research experiment was conducted to examine the effect of Rhizobium inoculation (RI) and P fertilization (P) on nodulation, growth and yield characteristics of soybean grown in the presence of starter N fertilizer (N). Treatments included: i) 02 levels of RI (RI 0, RI 1 ), ii) 02 levels of P (P 0 , P 1 that is, P 2 O 5 @ 90 kg ha -1 ) iii) 03 levels of N (N 0 , N 1 and N 2 that is, N at of 25 and 50 kg N ha -1 ) iv) 03 replication. Results of the experiment revealed that total number of nodules increased from 73 in the un-inoculated control to 125 and 95 following the application of RI and P representing 70 and 30% increase over control. N supply did not affect the number of nodules; however, combination of RI and P with 25 kg N ha -1 (RI 1 P 1 N 1 ) produced the highest number of nodules (152). RI, P, N and their combinations increased shoot and root biomass. Seed yield in the control was 767 kg ha -1 that significantly increased to 1081, 907 and 940 kg ha -1 following the application of RI, P and N demonstrating a 41, 18 and 23% increase over control. The highest seed yield of 1208 kg ha -1 was recorded in the combine treatment of RI 1 P 1 N 1 indicating 57% increase over control. Relative increase in dry matter yield due to RI, P and N was 63, 46 and 49%. Seed protein content in different treatments ranged between 33 -40% while oil content ranged between 13 -18%. Application of RI, P and their combinations increased protein content by 6 -22% while increase in oil content was 12 -35%. Concentrations of N and P in plants and their uptake was significantly increased and relative increase in N uptake due to RI, P and K was 77, 21 and 31% , respectively, while the corresponding increase in P uptake was 79, 92 and 56%. It was found that the efficiency of RI and P fertilization increased substantially with the application of 25 kg N ha -1 but the efficiency decreased when N supply increased from 25 kg N ha -1 to 50 kg N ha -1 . The results demonstrate the potential benefits of using Rhizobium inoculation and P fertilization with reduced level of N as starter fertilizer in order to achieve plant-growth promotion, increased nodulation and seed yield of soybean.

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Field evaluation of lentil cultivars inoculated with Rhizobium leguminosarum bv. viciae strains for nitrogen fixation using nitrogen-15 isotope dilution

Cited by 31 publications

References 15 publications

Potential of indigenous bradyrhizobia versus commercial inoculants to improve cowpea (Vigna unguiculataL. walp.) and green gram (Vigna radiataL. wilczek.) yields in Kenya

Potential of indigenous bradyrhizobia versus commercial inoculants to improve cowpea (Vigna unguiculataL. walp.) and green gram (Vigna radiataL. wilczek.) yields in Kenya

Residual effect of nitrogen fixed by mungbean (Vigna radiata) and blackgram (Vigna mungo) on subsequent rice and wheat crops

Effect of Rhizobium inoculation and NP fertilization on growth, yield and nodulation of soybean (Glycine max L.) in the sub-humid hilly region of Rawalakot Azad Jammu and Kashmir, Pakistan

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