Field Evaluation and Molecular Analysis of Bean Genotypes for Resistance to Rust Disease

El-Awady, Mohamed A.; Hamed, A. A.

doi:10.21608/ejgc.2015.9707

Cited by 2 publications

(2 citation statements)

References 32 publications

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“…Consequently, it was concluded that Neraska, Bronco, Dicola, Calypso and Samantha, had the potential to decrease the amount of bean rust infection in both seasons of the study. The obtained results are in agreement with EL-Awady and Hamed [ 38 ] who mentioned that 22 cultivated genotypes were evaluated naturally against rust pathogen, where the Samantha genotype was resistant; however, four genotypes Concessa, Hort. 440, Coby and Hana were immune.…”

Section: Discussionsupporting

confidence: 90%

Host Resistance to Uromyces appendiculatus in Common Bean Genotypes

Omara

Kamel

El-Ganainy

et al. 2022

Plants

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Rust, induced by the fungus Uromyces appendiculatus, is one of the most serious bean diseases. The mechanisms involved in rust resistance were evaluated in 10 common bean genotypes during the 2019/2020 and 2020/2021 growing seasons. The disease parameters, such as final rust severity (FRS%), area under the disease progress curve (AUDPC) and disease increase rate (r-value), were lower in the resistant genotypes than in highly susceptible genotypes. Biochemical compounds such as phenols, and the activity of antioxidant enzymes such as catalase, peroxidase and polyphenol oxidase were increased in the plants with resistant genotypes compared to those with susceptible genotypes. In the resistant genotypes, the levels of oxidative stress markers such as hydrogen peroxide (H2O2) and superoxide (O2•−) increased dramatically after infection. The electrolyte leakage percentage (EL%) was found to be much greater in susceptible genotypes than resistant genotypes. The resistance gene SA14, which was found in genotypes Nebraska and Calypso at 800 bp, provided an adequate level of resistance to bean rust, showing high grain yield potential. After infection, the transcription levels of 1,3-D-glucanases and phenylalanine ammonia lyase were higher in the resistant genotypes than in the susceptible genotypes. In conclusion, the resistant genotypes successfully displayed desirable agronomic traits and are promising candidates for breeding programs aiming to improve management strategies of common bean rust disease. The resistance was mediated by antioxidant enzymes, phenolic compounds and the expression of defense genes and the resistance gene SA14.

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Section: Discussionsupporting

confidence: 90%

Host Resistance to Uromyces appendiculatus in Common Bean Genotypes

Omara

Kamel

El-Ganainy

et al. 2022

Plants

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“…At the same time, this is especially important in the Middle East and North Africa, where moderate to large losses can occur if the disease starts early (El-Fawy andAbo-Elyousr, 2016 andIsmail andAfifi, 2019). Several investigators reported bean rust (Uromyces appendiculatus) as a widely spread disease in Egypt causing yield losses of dry and snap bean productions up to 70% (Abdalla, 1979;El-El-Awady and Hamed, 2015;El-Fawy and Abo-Elyousr, 2016;Ismail and Afifi, 2019;Said andTaher, 2020 andOmara et al 2022).…”

Section: Discussionmentioning

confidence: 99%

Potential of Biotic and Abiotic Inducers of Eliciting Systemic Resistance in Snap Bean (Phaseolus vulgaris L.) in Controlling Rust Caused by Uromyces appendiculatus

Basiony,

Amer,

El-Kafrawy

et al. 2023

Egyptian Journal of Phytopathology

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The biotic and abiotic inducers of resistance i.e., Bacillus subtilis, Paenibacillus polymyxa, Trichoderma harzianum, Trichoderma viride, ascorbic acid, Bion, potassium citrate, salicylic acid, as well the fungicides i.e., Domark EC 10% and Tilt EC 25%, were evaluated in vitro, and field conditions in both 2019 and 2020 growing seasons to control snap bean rust (Uromyces appendiculatus) at Ashmoun, Menoufia Governorate, Egypt. In vitro results indicated that all the tested bio-agents, chemical resistance inducers and the two fungicides significantly inhibited uredospores germination. The fungicide Domark EC 10% was the best effective treatment followed by Tilt EC 25% fungicide and B. subtilis, where they recorded 97.1, 96.3 and 82.4% inhibition of uredospore germination percentage, respectively, comparing with control treatment. Spraying the tested biotic, abiotic inducers, and the tested fungicides of bean plants under field conditions significantly reduced disease severity of bean rust, Area Under Disease Progress Curve (AUDPC) and rate of disease increase (r-value). Meanwhile, significantly increased performances of growth and yield parameters i.e., plant height, No. of branches/plant, plant fresh and dry weights, pod length (cm), average pod weight/plant (g) and yield (ton/fed), in both 2019 and 2020 growing seasons compared to the untreated control treatment. Meantime, considerable increases were recorded resulted in all the tested treatments in photosynthetic pigments, chlorophyll and carotenoids; activities of peroxidase (PO) polyphenol oxidase (PPO) and Catalase (CA) enzymes; phenol content, i.e., free phenols, conjugated phenols, and total phenols. Furthermore, a remarkable increase was recorded in the green pods chemical composition in protein and carbohydrates content (%) and Nitrogen (N), Phosphorus (P) and Potassium (K) minerals content compared to the untreated control.

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Field Evaluation and Molecular Analysis of Bean Genotypes for Resistance to Rust Disease

Cited by 2 publications

References 32 publications

Host Resistance to Uromyces appendiculatus in Common Bean Genotypes

Host Resistance to Uromyces appendiculatus in Common Bean Genotypes

Potential of Biotic and Abiotic Inducers of Eliciting Systemic Resistance in Snap Bean (Phaseolus vulgaris L.) in Controlling Rust Caused by Uromyces appendiculatus

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