Field emission scanning electron microscopy (FE-SEM) as an approach for nanoparticle detection inside cells

Havrdová, Markéta; Poláková, Kateřina; Skopalík, Josef; Vůjtek, Milan; Mokdad, Audrey; Homolkova, M.; Tuček, Jiří; Nebesářová, Jana; Zbořil, Radek

doi:10.1016/j.micron.2014.08.001

Cited by 35 publications

(18 citation statements)

References 27 publications

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“…Havrdova [ 125 ] first reported the potentiality of FE-SEM for imaging superparamagnetic iron oxide nanoparticles inside hMSCs. FE-SEM allows us to better observe NPs inside the cells but avoids the complex procedure including contrast staining and metal coating.…”

Section: Methods For Qualitative and Quantitative Analysis Of Cellmentioning

confidence: 99%

“…FE-SEM allows us to better observe NPs inside the cells but avoids the complex procedure including contrast staining and metal coating. Further, the researchers demonstrated that nanoparticles inside cells can be mapped using FE-SEM as the nanoparticles protrude through the membrane during the dehydration and drying sample preparation steps [ 125 ].…”

Section: Methods For Qualitative and Quantitative Analysis Of Cellmentioning

confidence: 99%

See 1 more Smart Citation

Intracellular Transport of Silver and Gold Nanoparticles and Biological Responses: An Update

Panzarini

Mariano

Carata

et al. 2018

IJMS

101

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Medicine, food, and cosmetics represent the new promising applications for silver (Ag) and gold (Au) nanoparticles (NPs). AgNPs are most commonly used in food and cosmetics; conversely, the main applications of gold NPs (AuNPs) are in the medical field. Thus, in view of the risk of accidentally or non-intended uptake of NPs deriving from the use of cosmetics, drugs, and food, the study of NPs–cell interactions represents a key question that puzzles researchers in both the nanomedicine and nanotoxicology fields. The response of cells starts when the NPs bind to the cell surface or when they are internalized. The amount and modality of their uptake depend on many and diverse parameters, such as NPs and cell types. Here, we discuss the state of the art of the knowledge and the uncertainties regarding the biological consequences of AgNPs and AuNPs, focusing on NPs cell uptake, location, and translocation. Finally, a section will be dedicated to the most currently available methods for qualitative and quantitative analysis of intracellular transport of metal NPs.

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Section: Methods For Qualitative and Quantitative Analysis Of Cellmentioning

confidence: 99%

Section: Methods For Qualitative and Quantitative Analysis Of Cellmentioning

confidence: 99%

Intracellular Transport of Silver and Gold Nanoparticles and Biological Responses: An Update

Panzarini

Mariano

Carata

et al. 2018

IJMS

101

View full text Add to dashboard Cite

show abstract

“…Although scanning electron microscopy is a powerful technique to characterize size, shape and structure of materials, when using backscattered electrons only the surface of samples can be evaluated [25] [26]. However, SEM analyses of fibers obtained by coaxial electrospinning of PLA and PVA solutions showed that some fibers presented cracks of the PLA shell, exposing the internal PVA fiber, as shown in Figure 4.…”

Section: Core-shell Fibers Structurementioning

confidence: 99%

Morphology and Thermal Properties of Core-Shell PVA/PLA Ultrafine Fibers Produced by Coaxial Electrospinning

Gonçalves¹,

Silva²,

Picciani³

et al. 2015

MSA

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Coaxial electrospinning process was used to produce biodegradable membranes made of coreshell fibers of a poly(lactic acid) (PLA) shell and a poly(vinyl alcohol) (PVA) core. Scanning electron microscopy analyses of these structures showed that the PLA shell can present certain porosity depending on the process condition. FTIR-ATR and contact angle measurements also suggested imprisonment of the PVA core within the PLA shell. This type of structure was also confirmed by means of transmissions electron microscopy. The morphology of these fibers was dependent on the flow rate of both core and shell solutions, and homogeneous and smooth surface was only attained when the flow rate of the external PLA solution was 4 times the flow rate of the internal PVA solution. The increase in the PLA solution flow rate increases the diameter of the core-shell fiber which reaches up to 1.7 μm. Nevertheless, fibers with smaller average diameter could also be produced (200 nm). These core-shell fibers presented improved hydrophilicity as compared with monolithic PLA fibers.

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“…Due to the small de Broglie wavelength of accelerated electrons, electron microscopy (EM) provides superior spatial resolution (<0.2 nm for TEM; ± 1 nm for SEM) and is the technique of choice when it comes to resolving structures that are below the optical resolution limit [22,63,64]. Thus, EM is frequently used as a third technique to evaluate [65][66][67][68][69] or quantify [70][71][72] cellular uptake. Rothen-Rutishauser et al compared uptake of AuNPs in A549 cells via CLSM and TEM.…”

Section: Figure 2 Strategies To Distinguish Between Membrane-bound Anmentioning

confidence: 99%

Methodologies to investigate intracellular barriers for nucleic acid delivery in non-viral gene therapy

et al. 2018

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A plethora of biological barriers, intended to defend tissues and cells against external influences, stand in the way of efficient nucleic acid delivery by non-viral nanoplexes. Even when nanoplexes successfully evade extracellular barriers and reach their target cell, many intracellular barriers remain to be conquered. These include overcoming the plasma membrane, evading endosomal compartmentalization, and in some cases crossing the nuclear envelope. At the same time, exocytosis, autophagy and cytoplasmic degradation of the cargo should be avoided. Currently, there is a growing appreciation that the interaction of nanoplexes with these barriers should be understood in detail in order to rationally design a second generation of non-viral nanoplexes, capable of overcoming these many hurdles. Studying intracellular biobarriers is, however, quite challenging and specialized methods are constantly being developed. In this review, we present an overview of established as well as emerging techniques and assays that are currently available to the experimentalist to study nanoplexbarrier interaction, with a focus on quantitative methods.

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Field emission scanning electron microscopy (FE-SEM) as an approach for nanoparticle detection inside cells

Cited by 35 publications

References 27 publications

Intracellular Transport of Silver and Gold Nanoparticles and Biological Responses: An Update

Intracellular Transport of Silver and Gold Nanoparticles and Biological Responses: An Update

Morphology and Thermal Properties of Core-Shell PVA/PLA Ultrafine Fibers Produced by Coaxial Electrospinning

Methodologies to investigate intracellular barriers for nucleic acid delivery in non-viral gene therapy

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