Field Efficacy and Transmission of Fast- and Slow-Killing Nucleopolyhedroviruses that Are Infectious to  Adoxophyes honmai (Lepidoptera: Tortricidae)

Takahashi, Mitsuaki; Nakai, M.; Saito, Yasumasa; Sato, Yasushi; Ishijima, Chikara; Kunimi, Yasuhisa

doi:10.3390/v7031271

Cited by 13 publications

(5 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Higher viral production ensures a higher transmission rate, so ‘fast‐killing’ viruses may controls pests more efficiently in the short term than ‘slow‐killing’ ones, but a ‘slow‐killing’ virus controls pests more effectively in the long term because of its higher transmission efficiency (Takahashi et al., ).…”

Section: Discussionmentioning

confidence: 99%

Potential of betabaculoviruses to control the tomato leafminer Tuta absoluta (Meyrick)

Valderrama

Barrera

López‐Ferber

et al. 2017

J Applied Entomology

View full text Add to dashboard Cite

The tomato leafminer Tuta absoluta (Meyrick 1917) (Lepidoptera:Gelechiidae) is a devastating pest, causing losses of up to 100%. An interesting tool for its control is the use of the insect specific viruses of Baculoviridae family. Despite its high potential, its use on T. absoluta larvae has been poorly studied. In this work, Colombian granuloviruses VG013 and VG003 isolated from T. absoluta and Tecia solanivora (Lepidoptera:Gelechiidae) larvae sampled in tomato and potato crops, respectively, were morphologically, biologically and molecularly characterized. Occlusion bodies showed ovoid shape containing one nucleocapsid. Restriction endonuclease analysis revealed a pattern similar to Phthorimaea operculella granulovirus and bioinformatics studies showed that both isolates are variants of that baculovirus specie. Similar mean lethal concentrations (LC50) on T. absoluta larvae were estimated for both viruses, although VG013 exhibited shorter mean time to death than VG003. The latter reached a higher OBs yield in comparison with VG013. These results demonstrated an interesting potential of evaluated betabaculoviruses to control T. absoluta populations and pointed key features to its use under field conditions.

show abstract

Section: Discussionmentioning

confidence: 99%

Potential of betabaculoviruses to control the tomato leafminer Tuta absoluta (Meyrick)

Valderrama

Barrera

López‐Ferber

et al. 2017

J Applied Entomology

View full text Add to dashboard Cite

show abstract

“…Infection by baculoviruses that carry the egt gene extends the larval period and increases virus yield in a host developmental stagedependent manner (Katsuma & Shimada, 2015), and increases virus transmission in the field (Cory et al, 2001). The larva-to-larva transmission rate of the virus in the next generation is also increased by the slower killing speed (Takahashi et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Entomopoxvirus infection induces changes in both juvenile hormone and ecdysteroid levels in larval Mythimna separata

Nakai

Kinjo

Takatsuka

et al. 2016

Journal of General Virology

Self Cite

View full text Add to dashboard Cite

Insect viruses are among the most important pathogens of lepidopteran insects. Virus-infected larvae often show developmental defects including a prolonged larval period and a failure to pupate, but the mechanisms by which insect viruses regulate host development need further investigation. In this study, the regulation of host endocrinology by a lepidopteran entomopoxvirus (EPV), Mythimna separata EPV (MySEV), was examined. When fourth instar M. separata were inoculated with MySEV occlusion bodies, pupation was prevented and the insects died during the final (sixth) larval instar. Liquid chromatography-MS analysis revealed that juvenile hormone (JH) titres in the haemolymph of MySEV-infected sixth instars were higher than those in mock-infected larvae. JH esterase (JHE) activity was also examined by kinetic assay using a colorimetric substrate. The level of JHE activity in the haemolymph of MySEVinfected larvae was generally lower than that found in mock-infected larvae. In contrast, ecdysteroid titre in the haemolymph of final-instar MySEV-infected larvae was lower than that found in mock-infected larvae when measured by radioimmunoassay. A statistically significant difference in the release of ecdysteroids from prothoracic glands (PGs) that were dissected from MySEV-or mock-infected sixth instar Day 3 larvae was not found following prothoracicotropic hormone (PTTH) exposure. Our results indicate that the release of ecdysteroids was reduced not by infection of the PGs by MySEV, but by reduced PTTH production from the brain. Taken together our study suggests that EPVs retard host development by both reducing ecdysone titre and maintaining status quo levels of JH by preventing its metabolism.

show abstract

“…Type I GVs present a slow insecticidal activity due to require a long time (15-37 d, depending on dose and larval instar of host) to cause symptomatic infection or death of host. This type of viruses are known as slow-killing and can cause a horizontal transmission, which has higher e cacy for long-term control of insect pests since they can infect different larval instars, prolongs larval development, causing the kill in nal larval instars with the consequent greater number of infective OBs produced (Hatem et al 2011;Hilton and Winstanley 2008;Takahashi et al 2015). Type I GVs rstly infect the midgut epithelium and, then, the infection migrates to the fatty tissue of the insect, with this tissue being the most important infection site (Federici 1997;Sciocco-Cap 2001).…”

Section: Introductionmentioning

confidence: 99%

Morphological, biological, and molecular characterization of Type I granuloviruses of Spodoptera frugiperda

Ordóñez-García,

Bustillos-Rodríguez,

Ornelas-Paz

et al. 2024

Preprint

View full text Add to dashboard Cite

The granuloviruses or GVs (Betabaculovirus) associated with the fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), especially those of Type I, have scarcely been studied but they might represent an alternative for the biocontrol of this insect. In this study, the native granuloviruses SfGV-CH13 and SfGV-CH28 isolated from FAW larvae were characterized for morphology, molecular traits, and insecticidal activity. The elapsed time between symptomatic infection of larvae and stop feeding as well as the weight of larvae before death or prior to pupation were also evaluated. Both granuloviruses isolates showed ovoid shape with a length of 0.4 µm. They showed the same DNA restriction profiles and their genome sizes were about 126 kb. The symptomatic infection with tested GVs mainly caused flaccidity of larva body and discoloration of integument. The integument lysis was only observed in 8% of infected larvae. Infected larvae gradually stopped feeding. Overall, these symptoms are characteristic of infections caused by Type I granuloviruses, which are known as monoorganotropic or slow-killing. The median lethal doses (LD50) values for SfGV-CH13 and SfGV-CH28 isolates were 5.4 × 102 and 1.1 × 103 OBs/larva, respectively. The median lethal time (LT50) ranged from 17 to 24 d. LT50 values decreased as the viral dose was increased. The elapsed time since symptomatic infection until pupation (LD50) and body weight of larvae (third instar) were higher with SfGV-CH28 than SfGV-CH13. Both granulovirus isolates were able to kill the FAW larvae from the 12th day.

show abstract

Field Efficacy and Transmission of Fast- and Slow-Killing Nucleopolyhedroviruses that Are Infectious to Adoxophyes honmai (Lepidoptera: Tortricidae)

Cited by 13 publications

References 34 publications

Potential of betabaculoviruses to control the tomato leafminer Tuta absoluta (Meyrick)

Potential of betabaculoviruses to control the tomato leafminer Tuta absoluta (Meyrick)

Entomopoxvirus infection induces changes in both juvenile hormone and ecdysteroid levels in larval Mythimna separata

Morphological, biological, and molecular characterization of Type I granuloviruses of Spodoptera frugiperda

Contact Info

Product

Resources

About