Field‐Driven Biofunctionalization of Polymer Fiber Surfaces during Electrospinning

Sun, Xiaoyu; Shankar, Ravi; Börner, Hans G.; Ghosh, Tushar K.; Spontak, Richard J.

doi:10.1002/adma.200601345

Cited by 112 publications

(79 citation statements)

References 47 publications

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“…The high protein release from scaffolds of 100 mg/ml PRGF over the other scaffolds may be explained by the scaffold's smaller fiber diameters. Although not specifically investigated in this study, other previously published studies have demonstrated similar results, and explain that with smaller fiber diameters there is less distance for molecules to traverse to reach the fiber surface, hence, more protein release from the fibers over time [46][47][48][49]. The rise in protein release after day 21 may be due to fiber degradation of the PRGF scaffolds occurring around 28-35 days and subsequent release of entrapped proteins.…”

Section: Protein Release Kineticssupporting

confidence: 79%

The Creation of Electrospun Nanofibers from Platelet Rich Plasma

Wolfe¹,

Sell²,

Ericksen³

et al. 2011

J Tissue Sci Eng

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Activated platelet rich plasma (a PRP) contains supra physiologic amounts of autologous growth factors and cytokines known to enhance wound healing and tissue regeneration. Here we report the first results of electro spinning nanofibers from a PRP to create fibrous scaffolds that could be used for various tissue engineering applications. Human platelet rich plasma (PRP) was created, activated by a freeze-thaw-freeze process, and lyophilized to form a powdered preparation rich in growth factors (PRGF). It was dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol (HFP) at different concentrations to form fibers with average diameters of 0.3 -3.6 µm. A sustained release of protein from the PRGF scaffolds was demonstrated up to 35 days, and cell interactions with the PRGF scaffolds confirmed cell infiltration after just 3 days. As electro spinning is a simple process, and PRGF contains naturally occurring growth factors in physiologic ratios, creating nanofibrous structures from PRGF has the potential to be beneficial for a variety of tissue engineering applications.

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Section: Protein Release Kineticssupporting

confidence: 79%

The Creation of Electrospun Nanofibers from Platelet Rich Plasma

Wolfe¹,

Sell²,

Ericksen³

et al. 2011

J Tissue Sci Eng

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“…The use of PLA-T6 oligomers should enable a good blending between the P(L)LA matrix and the additive. Moreover, it has been reported that, during fibre formation, the charge applied to the jet promotes the migration of the polarizable species contained in the solution (PLA-T6 in this case) towards the surface of the fibre [118,119].…”

Section: Surface Functionalizationmentioning

confidence: 88%

“…Since then gas foaming has become an appealing technique for fabricating microporous scaffolds [118,119].…”

Section: Gas Foamingmentioning

confidence: 99%

“…The method exploits the unique properties of scCO 2 that, combining liquid-like densities (high solvent power) with gas-like viscosities (high diffusion rates) [118], is used for a wide range of applications in polymer synthesis, extraction and impregnation processes, particle formation and blending [120,121]. Moreover, CO 2 has a relatively low critical point (T c = 31 °C, P c = 7.4 MPa) that can be easily achieved in a high-pressure equipped laboratory.…”

Section: Gas Foamingmentioning

confidence: 99%

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Porous Polymeric Bioresorbable Scaffolds for Tissue Engineering

Gualandi

2011

Springer Theses

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and some lactide copolymers) and of non-commercial polyesters (i.e. poly-ω-pentadecalactone and some of its copolymers) were explored and discussed.Two techniques were employed to fabricate scaffolds: supercritical carbon dioxide (scCO 2 ) foaming and electrospinning (ES). The former is a powerful technology that enables to produce 3D microporous foams by avoiding the use of solvents that can be toxic to mammalian cells. The scCO 2 process, which is II commonly applied to amorphous polymers, was successfully modified to foam a highly crystalline poly(ω-pentadecalactone-co-ε-caprolactone) copolymer and the effect of process parameters on scaffold morphology and thermo-mechanical properties was investigated.In the course of the present research activity, sub-micrometric fibrous nonwoven meshes were produced using ES technology. Electrospun materials are considered highly promising scaffolds because they resemble the 3D organization of native extra cellular matrix. A careful control of process parameters allowed to fabricate defect-free fibres with diameters ranging from hundreds of nanometers to several microns, having either smooth or porous surface. Moreover, versatility of ES technology enabled to produce electrospun scaffolds from different polyesters as well as "composite" non-woven meshes by concomitantly electrospinning different fibres in terms of both fibre morphology and polymer material. The 3D-architecture of the electrospun scaffolds fabricated in this research was controlled in terms of mutual fibre orientation by properly modifying the instrumental apparatus. This aspect is particularly interesting since the micro/nano-architecture of the scaffold is known to affect cell behaviour.Since last generation scaffolds are expected to induce specific cell response, the present research activity also explored the possibility to produce electrospun scaffolds bioactive towards cells. Bio-functionalized substrates were obtained by loading polymer fibres with growth factors (i.e. biomolecules that elicit specific cell behaviour) and it was demonstrated that, despite the high voltages applied during electrospinning, the growth factor retains its biological activity once

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“…[20][21][22][23][24] An alternative means by which to achieve surface modification or produce a core-sheath morphology relies on the surface segregation of a homopolymer or a functional species within an electrospun polymer blend due to surface energy, electric field or phase separation considerations. [23,25,26] In our previous study, [27] we have proposed a polarizability contrast strategy for in situ fielddriven surface modification of electrospun fibers during electrospinning. A peptide-polymer conjugate, composed of three repeats of a triad of serine, glutamic acid and glutamic acid ((Ser-Glu-Glu) 3 or (SEE) 3 ) connected to a poly(ethylene oxide) (PEO) block (M n ¼ 3 200 g Á mol…”

Section: Introductionmentioning

confidence: 99%

Field‐Driven Surface Segregation of Biofunctional Species on Electrospun PMMA/PEO Microfibers

Sun

Nobles

Börner

et al. 2008

Macromol. Rapid Commun.

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The need to biofunctionalize polymer surfaces for targeted bio‐related applications continues to grow, and efforts designed to meet this need rely heavily on surface grafting or polymerization. In this study, we provide a viable alternative by demonstrating that the peptide segment of a polymer‐peptide conjugate can be selectively driven to the surface of polymer nano/microfibers during electrospinning due to contrast in polarizability. Judicious choice of the polymer sequence in the conjugate permits use of the conjugate with compatible fiber‐forming polymers. Here, we use a water soluble poly(ethylene oxide)‐containing conjugate in combination with a hydrophobic thermoplastic, poly(methyl methacrylate). Surface enrichment is measured by X‐ray photoelectron spectroscopy, and fiber morphology is investigated by electron microscopy. Microfibers generated from the blends examined here are largely resistant to long term water immersion and are thus suited as support scaffolds or filtration membranes.magnified image

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Field‐Driven Biofunctionalization of Polymer Fiber Surfaces during Electrospinning

Cited by 112 publications

References 47 publications

The Creation of Electrospun Nanofibers from Platelet Rich Plasma

The Creation of Electrospun Nanofibers from Platelet Rich Plasma

Porous Polymeric Bioresorbable Scaffolds for Tissue Engineering

Field‐Driven Surface Segregation of Biofunctional Species on Electrospun PMMA/PEO Microfibers

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