Field Deployment of a Mobile Biosafety Laboratory Reveals the Co-Circulation of Dengue Viruses Serotype 1 and Serotype 2 in Louga City, Senegal, 2017

Dieng, Idrissa; Diarra, Maryam; Diagne, Moussa Moïse; Faye, Martin; Ndione, Marie Henriette Dior; Bâ, Yamar; Diop, Mamadou; Ndiaye, El Hadji; Zanotto, Paolo Marinho de Andrade; Diop, Boly; Ndiaye, Mamadou; Bousso, Abdoulaye; Dia, Ndongo; Diallo, Mawlouth; Barry, Aliou; Fall, Gamou; Loucoubar, Cheikh; Sall, Amadou Alpha; Faye, Ousmane

doi:10.1155/2021/8817987

Cited by 15 publications

(43 citation statements)

References 51 publications

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“…40-0700-24) and the Lightcycler 480 (Roche, Penzberg, Germany). This system allows simultaneous detection of four dengue virus serotypes from 5 μl of input RNA [ 26 ].…”

Section: Methodsmentioning

confidence: 99%

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Multifoci and multiserotypes circulation of dengue virus in Senegal between 2017 and 2018

et al. 2021

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Background Dengue fever is a mosquito born disease associated with self-limited to life threatening illness. First detected in Senegal in the nineteenth century, and despite its growing incidence this last decade, significant knowledge gaps exist in our knowledge of genetic diversity of circulating strains. This study highlights the circulating serotypes and genotypes between January 2017 and December 2018 and their spatial and temporal distribution throughout all regions of Senegal. Methods We used 56 dengue virus (DENV) strains for the analysis collected from 11 sampling areas: 39 from all regions of Senegal, and 17 isolates from Thiès, a particular area of the country. Two real time RT-qPCR systems were used to confirm dengue infection and corresponding serotypes. For molecular characterization, CprM gene was sequenced and submitted to phylogenetic analysis for serotypes and genotypes assignment. Results Three dengue virus serotypes (DENV-1–3) were detected by all used methods. DENV-3 was detected in 50% (28/56) of the isolates, followed by DENV-1 and DENV-2, each representing 25% (14/56) of the isolates. DENV-3 belongs to genotype III, DENV-1 to genotype V and DENV-2 to Cosmopolitan genotype. Serotype 3 was detected in 7 sampling locations and a co-circulation of different serotypes was observed in Thiès, Fatick and Richard-toll. Conclusions These results emphasize the need of continuous DENV surveillance in Senegal to detect DENV cases, to define circulating serotypes/genotypes and to prevent the spread and the occurrence of severe cases.

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“…40-0700-24) and the Lightcycler 480 (Roche, Penzberg, Germany). This system allows simultaneous detection of four dengue virus serotypes from 5 μl of input RNA [ 26 ].…”

Section: Methodsmentioning

confidence: 99%

“…In Senegal, the first dengue infection was reported in 1970 [23]. Since then, many outbreaks and sporadic cases were reported [24][25][26]. Between 2017 and 2018, an unprecedented number of cases associated to this viruses (DENV 1-3) occurred in Senegal [27,28].…”

Section: Introductionmentioning

confidence: 99%

Multifoci and multiserotypes circulation of dengue virus in Senegal between 2017 and 2018

et al. 2021

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“…During the last decade, all four DENV serotypes have been found in different regions of Senegal. In some epidemics, a co-circulation of two DENV serotypes was observed [ 44 , 48 ]. In Mali, Ae .…”

Section: Discussionmentioning

confidence: 99%

“…aegypti aegypti is common and has been incriminated in recent dengue epidemics [40][41][42]. Dengue fever is endemic in Senegal [43][44][45][46][47][48][49][50]. During the last decade, all four DENV serotypes have been found in different regions of Senegal.…”

Section: Plos Neglected Tropical Diseasesmentioning

confidence: 99%

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Emergence of dengue virus serotype 2 in Mauritania and molecular characterization of its circulation in West Africa

et al. 2021

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The number of sporadic and epidemic dengue fever cases have reportedly been increasing in recent years in some West African countries, such as Senegal and Mali. The first epidemic of laboratory-confirmed dengue occurred in Nouakchott, the capital city of Mauritania situated in the Saharan desert, in 2014. On-site diagnosis of dengue fever was established using a rapid diagnostic test for dengue. In parallel, the presence of Aedes aegypti mosquitoes in the city was confirmed. The initial diagnosis was confirmed by RT-PCR, which showed that all samples from the 2014 dengue epidemic in Nouakchott were dengue virus serotype 2 (DENV-2). The whole genome or envelope protein gene of these strains, together with other DENV-2 strains obtained from travelers returning from West African countries to France between 2016 and 2019 (including two Mauritanian strains in 2017 and 2018), were sequenced. Phylogenetic analysis suggested a recent emergence of an epidemic strain from the cosmopolitan genotype belonging to West African cosmopolitan lineage II, which is genetically distinct from African sylvatic genotype. The origin of this DENV-2 lineage is still unknown, but our data seem to suggest a recent and rapid dispersion of the epidemic strain throughout the region. More complete genome sequences of West African DENV-2 are required for a better understanding of the dynamics of its circulation. Arboviral surveillance and outbreak forecasting are urgently needed in West Africa.

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Molecular characterization of circulating Dengue virus 2 during an outbreak in Northern Senegal's Saint‐Louis region in 2018

Dieng

Ndiaye

Ndione

et al. 2022

Journal of Medical Virology

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Globally, 390 million people are at risk of dengue infection and over the past 50 years, the virus incidence increased thirty‐fold. In Senegal, an unprecedented occurrence of outbreaks and sporadic cases have been noticed since 2017. In October 2018, an outbreak of Dengue virus 2 (DENV‐2) was reported in the north of Senegal affecting multiple areas including Saint‐Louis, Richard Toll, and Rosso which are located at the border with Mauritania. Of these 173 blood specimen samples collected from patients, 27 were positive for dengue by quantitative reverse transcription PCR (qRT‐PCR), and eight were serologically confirmed to be positive for DENV immunoglobulin M (IgM). Serotyping using qRT‐PCR reveals that isolates were positive for DENV‐2. A subset of DENV‐2 positive samples was selected and subjected to whole‐genome sequencing followed by phylogenetic analysis. Analysis of six nearly complete genome sequences revealed that the isolates belong to the cosmopolitan genotype and are closely related to the Mauritanian strains detected between 2017 and 2018 and those detected in many West African countries such as Burkina Faso or Cote d'Ivoire. Our results suggest a transboundary circulation of the DENV‐2 cosmopolitan genotype between Senegal and Mauritania and call for a need for coordinated surveillance of arboviruses between these two countries. Interestingly, a high level of homology between West African isolates highlights endemicity and calls for the set‐up of subregional viral genomic surveillance which will lead to a better understanding of viral dynamics, transmission, and spread across Africa.

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Field Deployment of a Mobile Biosafety Laboratory Reveals the Co-Circulation of Dengue Viruses Serotype 1 and Serotype 2 in Louga City, Senegal, 2017

Cited by 15 publications

References 51 publications

Multifoci and multiserotypes circulation of dengue virus in Senegal between 2017 and 2018

Multifoci and multiserotypes circulation of dengue virus in Senegal between 2017 and 2018

Emergence of dengue virus serotype 2 in Mauritania and molecular characterization of its circulation in West Africa

Molecular characterization of circulating Dengue virus 2 during an outbreak in Northern Senegal's Saint‐Louis region in 2018

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