Field Demonstration of Successful Bioaugmentation To Achieve Dechlorination of Tetrachloroethene To Ethene

Abstract

A laboratory microcosm study and a pilot scale field test were conducted to evaluate biostimulation and bioaugmentation to dechlorinate tetrachloroethene (PCE) to ethene at Kelly Air Force Base. The site groundwater contained about 1 mg/L of PCE and lower amounts of trichloroethene (TCE) and cis-1,2-dichloroethene (cDCE). Laboratory microcosms inoculated with soil and groundwater from the site exhibited partial dechlorination of TCE to cDCE when amended with lactate or methanol. Following the addition of a dechlorinating enrichment culture, KB-1, the chlorinated ethenes in the microcosms were completely converted to ethene. The KB-1 culture is a natural dechlorinating microbial consortium that contains phylogenetic relatives of Dehalococcoides ethenogenes. The ability of KB-1 to stimulate biodegradation of chlorinated ethenes in situ was explored using a closed loop recirculation cell with a pore volume of approximately 64,000 L The pilot test area (PTA) groundwater was first amended with methanol and acetate to establish reducing conditions. Under these conditions, dechlorination of PCE to cDCE was observed. Thirteen liters of the KB-1 culture were then injected into the subsurface. Within 200 days, the concentrations of PCE, TCE, and cis-1,2-DCE within the PTA were all below 5 microg/L, and ethene production accounted for the observed mass loss. The maximum rates of dechlorination estimated from field date were rapid (half-lives of a few hours). Throughout the pilot test period, groundwater samples were assayed for the presence of Dehalococcoides using both a Dehalococcoides-specific PCR assay and 16S rDNA sequence information. The sequences detected in the PTA after bioaugmentation were specific to the Dehalococcoides species in the KB-1 culture. These sequences were observed to progressively increase in abundance and spread downgradient within the PTA. These results confirm that organisms in the KB-1 culture populated the PTA aquifer and contributed to the stimulation of dechlorination beyond cDCE to ethene.

Identification of chlorinated solvents degradation zones in clay till by high resolution chemical, microbial and compound specific isotope analysis

“…Natural and enhanced degradation of chlorinated ethenes in high permeability deposits (aquifers) is well known Major et al, 2002;Scheutz et al, 2008). In low permeability media, such as clay tills, little is known about how degradation by reductive dechlorination distributes between preferential flow paths such as sand lenses and fractures and the clay till matrix.…”

Genome sequence of the chlorinated compound–respiring bacterium Dehalococcoides species strain CBDB1

“…Several microbial groups carry out respiratory reductive dechlorination 1 , but one genus, Dehalococcoides, seems particularly adapted to a unique niche, and the bacteria are only known to use chloroorganics as electron acceptors and hydrogen as an electron donor for growth. Several studies have now shown successful application of Dehalococcoides-containing cultures for in situ remediation of contaminated sites 2,3 . The recently described genome sequence of D. ethenogenes, strain 195 (ref.…”

A Vista for Microbial Ecology and Environmental Biotechnology

“…Consortia containing Dehalococcoides organisms have been successfully applied at field sites where the intrinsic microbiology was insufficient to sustain acceptable degradation rates (27 ). Knowing the key players (i.e., Dehalococcoides ) and understanding the microorganisms' ecology were critical for successful technology implementation.…”

“…Perhaps they seek a single "magic bullet". Up to now, direct, incontrovertible proof has been scarce, but it is beginning to emerge (27).…”