Field cage studies with a genetic sexing strain in the Mediterranean fruit fly, <i>Ceratitis capitata</i>

Robinson, A. S.; Cirio, U.; Hooper, G. H. S.; Capparella, M.

doi:10.1111/j.1570-7458.1986.tb00533.x

Cited by 33 publications

(17 citation statements)

References 9 publications

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“…The sterile insect technique (SIT) is one of the methods used to eradicate or control this insect. However, because males and females are released, the efficiency of the method is reduced because of the preferential mating among the released flies (Robinson et al 1986). Furthermore, the SIT cannot be applied in those countries where this technique is used in regular intervals to suppress rather than to eradicate the target population.…”

Section: Introductionmentioning

confidence: 98%

Improved stability of genetic sex-separation strains for the Mediterranean fruit fly, Ceratitis capitata

Franz¹,

Gencheva²,

Kerremans³

1994

Genome

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In the existing genetic sexing strains for the medfly, Ceratitis capitata, male recombination leads to breakdown of the sexing mechanism under mass rearing conditions. The rate of breakdown depends on the recombination frequency and on the fitness of the recombinants. We have tested two different sexing genes, white pupa and a temperature sensitive lethal, in combination with the translocation T(Y;5)30C. Both sexing strains broke down, although at very different rates. In the case of the white pupa strain, 3.5% recombinants were observed after rearing the strain for 15 generations. The second strain, utilizing white pupa and the temperature sensitive lethal as selectable markers, already reached a comparable level after six generations and was broken down completely in the ninth generation. In these strains the frequency of recombination is high because the breakpoint of T(Y;5)30C and the sexing gene(s) are far apart. To remedy the situation, we have isolated four new translocations with breakpoints located closer to the sexing genes. Mass rearing was simulated for several generations with strains based on these translocations and no breakdown was observed under the conditions used.

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Section: Introductionmentioning

confidence: 98%

Improved stability of genetic sex-separation strains for the Mediterranean fruit fly, Ceratitis capitata

Franz¹,

Gencheva²,

Kerremans³

1994

Genome

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“…The overwintenng medfly population is composed mainly of larvae utilizing bitter orange as a host. Procida island was chosen as an experimental area to study the field performance of sterilized males from genetic sexing strains (Robinson, 1989) in a cooperative programme between ENEA (Italian National Conmiittee for Research and Development of Nuclear Energy) and IAEA (International Atomic Energy Agency) for the control of medfly using the SIT method (Sterile Insect Technique) (Cirio eta!., 1987). A total of 20.2 million sterile males from the strain T-101 (Seibersdorf, IAEA Laboratory) were released on the island of Procida from April 14 to July 13, 1986.…”

Section: Introductionmentioning

confidence: 99%

Spatial and temporal differentiation in colonizing populations of Ceratitis capitata

et al. 1992

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Two ancestral populations (Kenya and Reunion), two Mediterranean (Procida and Sardinia) and one new American population (Guatemala) of Ceratitis capitata were examined by electrophoresis for genetic variability at 27 enzyme loci. Two ordination approaches (principal component analysis and a tree representation) and F-statistical analysis have been used to distinguish the various patterns of genetic variations and to infer the underline causes and their relative contribution to the total variation. Three main patterns of variation emerge from the data: geographical, annual and seasonal differentiation. A main part of intraspecific variability involves the differentiation of central (Kenya and Reunion) versus peripheral populations (the Meditterranean and the American populations). The analysis suggests that the genetic structure of these populations is correlated with the historical events of their colonization. The affinity of the Guatemalan population with the Kenyan one could be the result of a recent founding of this population from the source area (Africa). More ancient historical events of colonization characterize the two Mediterranean populations. Seasonal variation has been found in the Procida population and chiefly involves the Mpi locus. In the same population the genetic variation across years has a minimum in 1986 due to the release of sterile T-101 males.

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“…As expected, these strains have many DNA fragments (bands of hybridization) in common but, in addition, there are some notable RFLPs observable between the two strains. The two "Robinson" strains are both derived from a line bearing a translocation of a wildtype gene for pupal color to the Y chromosome (Robinson 1989). They differ only in that the Robinson (Hawaiian) strain has been outcrossed to a strain from the Hawaiian islands (also originally from Kula), while the Robinson (pure) has not been outcrossed.…”

Section: Resultsmentioning

confidence: 99%

Genetic variation between strains of the Mediterranean fruit fly, Ceratitis capitata, detected by DNA fingerprinting

Haymer¹,

McInnis²,

Arcangeli³

1992

Genome

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DNA fingerprinting has been used to detect genetic variation in the Mediterranean fruit fly, Ceratitis capitata. Three different probes have been identified that can be used to detect DNA restriction fragment length polymorphisms between strains of this species. The strains used in this study differ only in terms of their geographic origin or genetic background. One of the probes used is the bacteriophage vector M13, and the other two are repetitive sequences derived from the medfly genome based on a weak homology to M13. Within a strain, each probe produces a consistent restriction fragment profile that is not affected by the method or timing of DNA extraction. Between strains, when M13 is used as a probe, an average of 10% of the observable bands are polymorphic. Use of the medfly genomic sequences as a probe increases the proportion of polymorphic bands between strains up to 30%. The fact that genetic differences between even such closely related strains can be reliably detected by this method holds great promise for studies of insect pests including the ability to monitor the movements of pest species, determining the extent of genetic variation in pest populations, and in making identifications from otherwise unidentifiable material.

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Field cage studies with a genetic sexing strain in the Mediterranean fruit fly, Ceratitis capitata

Cited by 33 publications

References 9 publications

Improved stability of genetic sex-separation strains for the Mediterranean fruit fly, Ceratitis capitata

Improved stability of genetic sex-separation strains for the Mediterranean fruit fly, Ceratitis capitata

Spatial and temporal differentiation in colonizing populations of Ceratitis capitata

Genetic variation between strains of the Mediterranean fruit fly, Ceratitis capitata, detected by DNA fingerprinting

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