2020
DOI: 10.1002/jmv.25778
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Field applicable detection of hepatitis B virus using internal controlled duplex recombinase‐aided amplification assay and lateral flow dipstick assay

Abstract: Hepatitis B virus (HBV) is a widespread blood‐borne pathogen associated with the complication of liver cirrhosis and hepatocellular carcinoma, particularly in south‐east Asian and African countries where HBV is highly endemic and the budget and resources are limited. Therefore, simple, rapid, and portable field detection methods are crucial to efficiently control HBV infection. In this study, using heat‐treated DNA, we developed two‐field applicable detection assays for HBV based on recombinase‐aided amplifica… Show more

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Cited by 20 publications
(21 citation statements)
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“…The RAA requires only a simple thermostatic device (constant temperature 37–42°C) and a short reaction time (30 min). DNA amplification can be detected by gel electrophoresis, oligo chromatographic lateral flow dipstick (LFD) ( Bai et al., 2020 ; Xiong et al., 2020 ), or real-time fluorescence methods ( Wang J. et al., 2020 ; Wang W. et al., 2020 ), considered as a powerful detection means for Point of Care Test (POCT). Here, we developed a molecular POCT method for SARS-CoV-2 based on a reverse transcription RAA assay coupled with lateral flow dipstick (RT-RAA/LFD) conducted at 39.0°C for 30 min.…”
Section: Introductionmentioning
confidence: 99%
“…The RAA requires only a simple thermostatic device (constant temperature 37–42°C) and a short reaction time (30 min). DNA amplification can be detected by gel electrophoresis, oligo chromatographic lateral flow dipstick (LFD) ( Bai et al., 2020 ; Xiong et al., 2020 ), or real-time fluorescence methods ( Wang J. et al., 2020 ; Wang W. et al., 2020 ), considered as a powerful detection means for Point of Care Test (POCT). Here, we developed a molecular POCT method for SARS-CoV-2 based on a reverse transcription RAA assay coupled with lateral flow dipstick (RT-RAA/LFD) conducted at 39.0°C for 30 min.…”
Section: Introductionmentioning
confidence: 99%
“…In addition, a competitive IAC was integrated that excludes false negative results and, in combination with the flow control, ensures the validity of the test result. Until now only a part of the published RPA approaches incorporated an IAC [ 46 , 81 , 82 , 83 , 84 , 85 , 86 , 87 ]. The main advantages over the non-competitive method are: (1) avoiding the risk of undesired interaction of multiple primers and (2) a more accurate reflection of the amplification, due to the identical primers and reaction conditions.…”
Section: Discussionmentioning
confidence: 99%
“…Among the isothermal amplification methods that can be interacted with LFA-based analysis, especially the recombinase polymerase amplification (RPA) has gained in importance as a cost-effective and reliable technology for POC diagnostics [ 37 ]. In the last years several articles were published that successfully combined RPA and LFA [ 36 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 ]. However, only a minority of them showed direct amplification of target nucleic acids from crude lysate [ 45 , 46 , 47 , 48 , 49 , 50 ].…”
Section: Introductionmentioning
confidence: 99%
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“…The reaction is typically completed in approximately 30 min at 37-42 °C. RAA has been successfully applied in the detection of pathogens (Bai and Ma et al, 2020;Li and Yu et al, 2020;Wang and Cui et al, 2020; and single nucleotide polymorphisms (SNPs) (Duan and Li et al, 2018). Owing to its speed, low-cost, and high sensitivity, RAA is highly suitable for clinical applications, and is a potential assay for the point-of-care testing (POCT) for foodborne pathogens.…”
Section: Introductionmentioning
confidence: 99%