Field and laboratory transmission studies of haemic neoplasia in the soft‐shell clam, <i>Mya arenaria</i>, from Atlantic Canada

Mateo, Dante R.; MacCallum, G S; Davidson, Jeff

doi:10.1111/jfd.12426

Cited by 20 publications

(20 citation statements)

References 28 publications

(110 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Invertebrates do not possess a vertebrate-like adaptive immune system or a major histocompatibility complex, which vertebrates utilize for discriminating self from nonself. However, in most previously identified cases of BTN, the neoplasia has only been identified in the species of origin (Metzger et al, 2016; Metzger et al, 2015), and attempts to experimentally transfer neoplastic cells from M. trossulus or the soft-shell clam Mya arenaria into several bivalve species have only been successful when the transfer is between members of the same species (Kent et al, 1991; Mateo et al, 2016). The species in the Mytilus complex are able to hybridize, so the barrier to cross-species transmission may be relatively low.…”

Section: Discussionmentioning

confidence: 99%

A single clonal lineage of transmissible cancer identified in two marine mussel species in South America and Europe

Yonemitsu

Giersch

Polo-Prieto

et al. 2019

eLife

264

View full text Add to dashboard Cite

Transmissible cancers, in which cancer cells themselves act as an infectious agent, have been identified in Tasmanian devils, dogs, and four bivalves. We investigated a disseminated neoplasia affecting geographically distant populations of two species of mussels (Mytilus chilensis in South America and M. edulis in Europe). Sequencing alleles from four loci (two nuclear and two mitochondrial) provided evidence of transmissible cancer in both species. Phylogenetic analysis of cancer-associated alleles and analysis of diagnostic SNPs showed that cancers in both species likely arose in a third species of mussel (M. trossulus), but these cancer cells are independent from the previously identified transmissible cancer in M. trossulus from Canada. Unexpectedly, cancers from M. chilensis and M. edulis are nearly identical, showing that the same cancer lineage affects both. Thus, a single transmissible cancer lineage has crossed into two new host species and has been transferred across the Atlantic and Pacific Oceans and between the Northern and Southern hemispheres.

show abstract

Section: Discussionmentioning

confidence: 99%

A single clonal lineage of transmissible cancer identified in two marine mussel species in South America and Europe

Yonemitsu

Giersch

Polo-Prieto

et al. 2019

eLife

264

View full text Add to dashboard Cite

show abstract

“…Disseminated neoplasias of molluscs have only been transplanted to members of the same species (soft-shell clams, mussels, and others 5,6 ). Attempts to transfer M. arenaria neoplasia through water exposure to both M. arenaria and M. trossulus only resulted in transfer of disease to M. arenaria 26 , and injection of M. trossulus cancer cells into multiple bivalve species only resulted in engraftment in M. trossulus 27 . Our finding that multiple cancer lineages are most often found to spread within the original host species is consistent with these previous experiments, and suggests that there may be species-specific restriction factors that prevent engraftment into divergent hosts.…”

mentioning

confidence: 99%

Widespread transmission of independent cancer lineages within multiple bivalve species

Metzger

Villalba

Carballal

et al. 2016

Nature

199

270

View full text Add to dashboard Cite

Most cancers arise from oncogenic changes in the genomes of somatic cells, and while the cells may migrate by metastasis, they remain within that single individual. Natural transmission of cancer cells from one individual to another has been observed in two distinctive cases in mammals (Tasmanian devils1 and dogs2,3), but these are generally considered to be rare exceptions in nature. The discovery of transmissible cancer in soft-shell clams (Mya arenaria)4 suggested that this phenomenon might be more widespread. Here we analyzed disseminated neoplasia in mussels (Mytilus trossulus), cockles (Cerastoderma edule), and golden carpet shell clams (Polititapes aureus) and found that neoplasias in all three species are attributable to independent transmissible cancer lineages. In mussels and cockles, the cancer lineages are derived from their respective host species, but unexpectedly, cancer cells in P. aureus are all derived from Venerupis corrugata, a different species living in the same geographic area. No cases of disseminated neoplasia have thus far been found in V. corrugata from the same region. These findings show that transmission of cancer cells in the marine environment is common in multiple species, that it has originated many times, and that while most transmissible cancers were found spreading within the species of origin, cross-species transmission of cancer cells can occur.

show abstract

“…Whereas a variety of biotic and abiotic factors have been proposed (Barber 2004, Walker et al 2011, Carballal et al 2015, the etiology of disseminated neoplasia in M. arenaria is now well understood to in volve transmission of a clonal lineage of neoplastic cells from one clam to another (Metzger et al 2015, Mateo et al 2016. In moribund M. arenaria, neoplastic hemocytes exhibit high genomic copy number and high RNA ex-pression of the Steamer retrotransposon.…”

Section: Introductionmentioning

confidence: 99%

Evidence of horizontal transmission of the cancer-associated Steamer retrotransposon among ecological cohort bivalve species

Paynter

Metzger²,

Sessa³

et al. 2017

Dis. Aquat. Org.

View full text Add to dashboard Cite

Bivalve specimens from legacy frozen tissue collections, and others freshly obtained, were surveyed for the presence of the Steamer long terminal repeat (LTR)-retrotransposon associated with disseminated hemic neoplasia of the soft-shelled clam Mya areneria. Of 22 species investigated using primers for the pol region, only Atlantic M. arenaria, Atlantic and North Sea razor clams Ensis directus, and Baltic clams Macoma balthica from the North Sea were found to possess copies of Steamer in their genomes. Notably, close relatives like Mya truncata and Siliqua patula did not exhibit evidence of Steamer. Amplified Steamer sequences were uniformly identical in all M. areneria specimens, and were highly variable across specimens of E. directus. Variation in the latter included nucleotide polymorphisms among and within individuals as well as length variation in 2 specimens corresponding to the deletion of a predicted stable hairpin structure. Results implicate Atlantic razor clams as the proximal source for horizontal transmission of Steamer among ecologically similar yet markedly distantly related bivalves. The consequences of cross-species transmission of the Steamer retrotransposon are unknown, and the finding of Steamer in 3 bivalve species suggests that further spread is possible. KEY WORDS: Disseminated neoplasia · Hemic neoplasia · Retrotransposon · Steamer · Bivalvia · CancerResale or republication not permitted without written consent of the publisher Dis Aquat Org 124: [165][166][167][168] 2017 pression of the Steamer retrotransposon. Steamer is a long terminal repeat (LTR)-retrotrans poson in the Mag family of Ty3/Gypsy elements, complete with a single open reading frame encoding gag and pol genes. Retrotransposons are present in genomic DNA and replicate through reverse transcription of an mRNA transcript into DNA, followed by integration into a new location within the genomic DNA of a cell. As they are notably lacking a retroviral env gene, they are not expected to be able to transfer from cell to cell, as retroviruses do. Similar retrotransposons have been identified in the genomes of 3 other bivalve species known to have transmissible neoplasias, one of which seems to cross the species boundary (Metzger et al. 2016).To date, the Steamer retrotransposon itself is un ique to M. arenaria. Here we examined legacy bio diversity collections and freshly acquired bivalve tissues for the presence of a Steamer signature in their genomes in a manner that might (1) better elucidate whether disseminated neoplasias are ecologically or phylogenetically determined and (2) shed light on the potential for horizontal spread and genomic modification by Steamerlike retrotransposons in the marine environment. MATERIALS AND METHODSBivalve tissue samples were obtained from the American Museum of Natural History's Ambrose Monell Cryo Collection (AMCC), and included 22 species from the North Atlantic (see Table S1 in the Supplement at www. int-res. com/ articles/ suppl/ d124 p165 _ supp. pdf). Fresh specimens of steam...

show abstract

Field and laboratory transmission studies of haemic neoplasia in the soft‐shell clam, Mya arenaria, from Atlantic Canada

Cited by 20 publications

References 28 publications

A single clonal lineage of transmissible cancer identified in two marine mussel species in South America and Europe

A single clonal lineage of transmissible cancer identified in two marine mussel species in South America and Europe

Widespread transmission of independent cancer lineages within multiple bivalve species

Evidence of horizontal transmission of the cancer-associated Steamer retrotransposon among ecological cohort bivalve species

Contact Info

Product

Resources

About