Field and Laboratory Evaluation of Bioefficacy of an Insect Growth Regulator (Dimilin) as a Larvicide against Mosquito and Housefly Larvae

Abstract

The inhibitory function of Dimilin (Diflubenzuron), mostly a chitin synthesis regulator, on the ecdysis of mosquitoes (Anopheles gambiae s.l., Culex quinquefasciatus) and housefly was evaluated in the field and in laboratory. Three formulations of Diflubenzuron were evaluated in this study: Dimilin, Wettable powder (25%), Dimilin granules (2%), and Dimilin tablets (2%). The laboratory and field evaluation used different rates of concentrations of these formulations. Generally, at higher dosages larvae developments, eggs hatchability and pupation were impossible. The development of mosquitoes was significantly higher in control while highly depressed in different dosages of treatment in both laboratory and field experiments. In houseflies, the adult population decreased sharply after treatment of their breeding sites while pupae mortality was noticed to be high in laboratory-treated samples. Dimilin could be opted as one of the choice of the larval control chemicals to be incorporated in the integrated vector control programmes in urban and rural areas.

Statistical Evaluation Of The Larvicidal Effect Of Diflubenzuron On Culex Pipiens Larval Stages

“…This coincides with the results obtained by a number of authors working in different geographical regions [36][37][38]. The effect of 2% difl ubenzuron granules was determined in specifi c habitats [34].…”

Bio-efficacy and persistence evaluation of Dimilin<sup>®</sup>GR-2% and Mosquiron<sup>® </sup>10 EC insect growth regulators against <i>Anopheles gambiae</i> s.l (diptera: Culicidae) larvae

“…gambiae s.l was delayed up to 96 and 72 h and after treatment. Delayed effectiveness against larvae of Anopheles species has also been reported [27].…”

Efficacy of Entomopathogenic Fungi and Nematodes, and Low Risk Insecticides Against Wheat Stem Sawfly, Cephus cinctus (Hymenoptera: Cephidae)

“…Diflubenzuron (Dimilin) kills insects by interfering with chitin synthesis and disrupting insect growth. Dimilin has been shown to be effective in controlling larvae of Anopheles gambiae Giles (Diptera: Culcidae) (Msangi et al, 2011), Culex quinquefasciatus Say (Diptera: Culcidae) (Msangi et al, 2011), Musca domestica Linnaeus (Diptera: Muscidae) (Batra et al, 2005;Le Menach et al, 2007;WHO, 1984).…”

“…Vuill (McGuire et al, 2005;Bhadauria et al, 2013), Metarhizium anisopliae (Metschnikoff) Sorokin (Bhat et al, 2010;Larramendy et al, 2011), S. carpocapsae Weiser (Cossentine et al, 2002;Chambers et al, 2010), S. kraussei Steiner (Haukeland & Lola-Luz, 2010), S. feltiae Filipjev (Shapiro-Ilan et al, 2004;Navaneethan et al, 2010), and Heterorhabditis bacteriophora Poinar (Toledo et al, 2005;Koppenhöfer et al, 2006). Dimilin is an insect growth regulator which has been reported to be effective in mosquito control (Msangi et al, 2011). Neem (Aza-direct) has also been reported to be effective against some insects (Mamoon-ur-Rashid et al, 2013, Sivasakthi et al, 2013.…”