Field and in vitro assay of three methods for freezing ram semen

Anel, L.; Paz, P. de; Álvarez, M.; Chamorro, C.A.; Boixo, J.C.; Manso, Alfonso Fernández; González, Marco Aurélio Stumpf; Kaabi, M.; Anel, E.

doi:10.1016/s0093-691x(03)00140-7

Cited by 72 publications

(84 citation statements)

References 23 publications

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“…O uso de IA com sêmen fresco ou refrigerado, quando estes são comparados com sêmen congelado, apresenta maiores chances de popularização por requerer técnicas menos sofisticadas de deposição do sêmen no genital feminino (Bicudo et al, 2005).…”

Section: Introductionunclassified

Adição de plasma seminal ao sêmen descongelado e taxa de prenhez de ovelhas inseminadas em tempo fixo

Prado

Bastos

Monteiro

et al. 2013

Arq. Bras. Med. Vet. Zootec.

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Adição de plasma seminal ao sêmen descongelado e taxa de prenhez de ovelhas inseminadas em tempo fixo RESUMOAvaliou-se o efeito da adição de plasma seminal ovino ao sêmen descongelado sobre a taxa de prenhez de ovelhas em rebanho comercial. Cento e setenta e quatro ovelhas cruza Texel foram distribuídas em quatro tratamentos: T1) inseminação artificial cervical (IAC) com sêmen descongelado (SD) diluído em solução tampão fosfato salino (PBS); T2) IAC com SD e adição de plasma seminal ovino; T3) grupo-controle I: IAC com sêmen fresco diluído em PBS; T4) grupo-controle II: inseminação artificial por laparoscopia com SD diluído em PBS. Para indução de cio, utilizaram-se esponjas impregnadas com acetato de medroxiprogesterona (MAP) por 12 dias, com aplicação intramuscular de 400 UI de eCG (Novormon ® ) e de 37,5µg de cloprostenol sódico (Sincrocio ® ), no dia da retirada das esponjas. O aparecimento de cio foi monitorado com rufiões vasectomizados a partir da retirada das esponjas até a inseminação artificial em tempo fixo -54 a 60 horas. A taxa de prenhez do tratamento com adição de plasma seminal ao sêmen descongelado (7,0%) não diferiu (P>0,05) do tratamento sem adição de plasma (4,3%), entretanto foi menor (P<0,05) se comparada à taxa de prenhez dos grupos-controle I inseminação via cervical superficial com sêmen fresco diluído (50,0%) e II inseminação via laparoscopia com sêmen descongelado (39,4%). A inseminação artificial por via cervical superficial com adição de plasma seminal ao sêmen descongelado não elevou o percentual de prenhez em valores que justifiquem a indicação desta biotecnologia em rebanhos comerciais de ovinos.Palavras-chave: ovelha, inseminação cervical, laparoscopia ABSTRACT

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Section: Introductionunclassified

Adição de plasma seminal ao sêmen descongelado e taxa de prenhez de ovelhas inseminadas em tempo fixo

Prado

Bastos

Monteiro

et al. 2013

Arq. Bras. Med. Vet. Zootec.

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“…For this reason, freezing dam ag es mu st be r e duce d to im pr ove the fertilization rate and subsequent fertility by CI. Semen diluents for most animal species, including marine mammals, usually contain glucose or fructose, egg yolk, and glycerol [16][17][18][22][23][24]. However, preparation of uniform semen diluents containing egg yolk is difficult because the individual egg yolk quality may vary depending on the number of days after laying and the storage period.…”

mentioning

confidence: 99%

Effects of Bovine Serum Albumin and Trehalose in Semen Diluents for Improvement of Frozen-Thawed Ram Spermatozoa

Matsuoka

Imai

Kohno

et al. 2006

Journal of Reproduction and Development

104

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Abstract. In this study, two following experiments were performed to improve post-thaw motility and viability of frozen-thawed ram spermatozoa. We examined i) the effects of different concentrations of bovine serum albumin (0, 0.3, 1, 5, 10 and 15% BSA) in semen diluents lacking egg yolk and ii) the effects of four semen diluents, fructose (F: control) and trehalose (T) in semen diluents containing egg yolk, 15% BSA in semen diluents without egg yolk (BSA), and modified phosphate buffered saline (m-PBS). Frozen-thawed spermatozoa were examined for progressive sperm motility, viability, morphological abnormality, sperm tail swelling test, and sperm acrosome integrity. In Experiment 1, the rates of sperm motility immediately after thawing (0 h) were significantly (P<0.05) higher in the 10 and 15% BSA groups (55.0 ± 2.9 and 58.3 ± 6.7%, respectively) than in the positive control (F) group (41.7 ± 4.4%). The rate of sperm viability in the negative control (0% BSA) group (80.2 ± 3.3%) was significantly (P<0.05) lower than in the positive control (F) group (89.8 ± 1.5%), but when compared with the F group, no significant differences were found among the 0.3, 1, 5, 10 and 15% BSA groups at 0 h. The rates of sperm morphological abnormality of the 10 and 15% BSA groups (6.5 ± 1.3 and 6.3 ± 1.1%, respectively) were significantly (P<0.05) lower at 0 h than that in the 1% BSA group (16.3 ± 5.2%). In Experiment 2, T addition improved (P<0.05) the post-thaw motility compared with the F and BSA groups. Furthermore, at 3 and 6 h, the post-thaw motility of the T group (36.3 ± 2.4 and 25.0 ± 2.0%, respectively) was significantly (P<0.05) higher than in the BSA (26.3 ± 2.4 and 18.8 ± 1.3%, respectively) and F (28.8 ± 3.8 and 18.8 ± 2.4%, respectively) groups. The post-thaw sperm motility and viability in the m-PBS group were significantly (P<0.05) lower than those of the control (F), T, and BSA groups throughout all observation points. These results indicate that 10 and 15% BSA can be substituted for egg-yolk for ram semen diluent and that the addition of trehalose enhances motility and viability of ram spermatozoa after freezing and thawing. Key words: Bovine serum albumin (BSA), Semen diluent, Sheep, Spermatozoa, Trehalose (J. Reprod. Dev. 52: [675][676][677][678][679][680][681][682][683] 2006) t has been reported that frozen-thawed spermatozoa are greatly damaged during the freezing and thawing processes [1][2][3][4][5]. This damage may decrease sperm motility and the fertilization rate after artificial insemination (AI). Several semen analysis methods have been developed to evaluate the functional parameters of spermatozoa more objectively. The hypo-osmotic swelling test (HOS-test) has been applied to various species, such as dogs [6,7], cattle [8,9], horses [10][11][12], rams [13], minke whales (Balaenoptera bonarensis) [13] and humans [14,15]. It has been recognized that the HOS-Test is a simple and useful method with good reliability and repeatability for examination of the sperm plasma membrane.

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“…They also reported that there was no significant difference between the CLC groups (0 and 4.5 mg) in functional membrane integrity between the treated groups at different G levels (3, 5, 7%). In the present study, 1.5mg/120x10 6 of 7-DHCLC and CLC were used with different ratios of G (3, 6, 9%) prior to freezing and after thawed HOS test was highest in 9% [34] have stated that the low level of CLC gives the possibility to decrease the required level of G for freezing. Although generally low level of G was found to preserve the volume of sperm cells and protect the cells from osmotic stress, in the current study, the highest total motility, viability and sperm membrane integrity values were observed in 9% G (high level G) group treated with 7-DHCLC when compared to other groups (P<0.001).…”

Section: Discussionmentioning

confidence: 93%

“…In most studies [35][36][37] , when cryoprotectant rate is increased in the freezing media, detrimental effects induced by osmotic stress become more visible. However, the higher survival rate of sperm treated with CLC could be due to the changes in osmotic tolerance and permeability of the membrane to G [34] . In the present study, besides the cholesterol, we loaded 7-dehydrocholesterol with cyclodextrin.…”

Section: Discussionmentioning

confidence: 99%

Kolestrol ve 7-Dehidrokolestrolün Farkli Gliserol Oranlari İle Boğa Spermasi Dondurulmasina Etkisi

̇İnanç¹,

Olğaç²,

Tekin³

et al. 2018

Kafkas Univ Vet Fak Derg

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How to Cite This Articleİnanç ME, Olgac KT, Tekin K, Çil B, Alemdar H, Özen D, Uysal O: Effect of cholesterol and 7-dehydrocholesterol on bull semen freezing with different rates of glycerol. Kafkas Univ Vet Fak Derg, 24 (6): 815-820, 2018. DOI: 10.9775/kvfd.2018 AbstractThe aim of this study was to evaluate the effect of 7-dehydrocholesterol loaded cyclodextrin (7-DHCLC) and cholesterol loaded cyclodextrin (CLC) with the addition of different glycerol (G) rates on bull semen cryopreservation. Semen samples of three bulls were pooled and divided into seven groups; control group (C) and the other six were extended with standard Tris extender including different G rates (3, 6 and 9%) with CLC or 7-DHCLC to reach 1.5 mg/120x10 6 . Extended samples were frozen in 0.25 mL straws. After thawing, motion characteristics and motility, viability, acrosome integrity, mitochondrial activation, functional membrane integrity and abnormal spermatozoa rates were assessed. The highest membrane integrity, viability and total motility were detected in 7-DHCLC with 9% of G group (P<0.001). The lowest progressive motility was detected in 3% G groups (P<0.001). 7-DHCLC with 9% G had higher mitochondrial activity compared with 3% G groups. In addition, no statistical difference was observed between groups in terms of acrosome integrity and abnormal spermatozoa rates (P>0.05). According to these results, 7-DHCLC with 9% G addition maintained some spermatological parameters better than other groups after thawing in bull semen thus, it was concluded that 7-DHCLC could be used instead of CLC in bull semen cryopreservation. Keywords: Cholesterol 7-dehydrocholesterol, Cyclodextrin, Glycerol, Bull semen, Freeze-thawed Kolestrol ve 7-Dehidrokolestrolün Farklı Gliserol Oranları İle Boğa Sperması Dondurulmasına Etkisi ÖzBu çalışmanın amacı, 7-dehidrokolesterol yüklü siklodekstrin (7-DHCLC) ve kolesterol yüklü siklodekstrin (CLC) 'in farklı gliserol (G) oranları ilavesi ile boğa sperması dondurulması üzerindeki etkilerini değerlendirmektir. Üç adet boğaya ait sperma örnekleri toplandı, birleştirildi ve yedi gruba ayrıldı; biri kontrol (C) ile, diğer altı grup ise CLC veya 7-DHCLC (1.5 mg/120x10 6 ) ve farklı G oranlarını (%3, 6 ve 9) içeren standart Tris sulandırıcısı ile sulandırıldı. Sulandırılan spermalar 0.25 mL'lik payetlerde donduruldu. Çözdürme sonrası gruplar hareket karakteristikleri ve motilite, viabilite, akrozom bütünlüğü, mitokondriyal aktivasyon, fonksiyonel membran bütünlüğü ve anormal spermatozoa oranları yönünden değerlendirildi. En yüksek membran bütünlüğü, viabilite ve motilite %9 G ilavesi ile 7-DHCLC grubunda tespit edildi (P<0.001). En düşük progresif motilite %3 G gruplarında saptandı (P<0.001). %9 G içeren 7-DHCLC grubu, %3 G gruplarına kıyasla daha yüksek mitokondriyal aktivite belirlendi. Ayrıca, gruplar arasında akrozom bütünlüğü ve anormal spermatozoa oranları açısından istatistiksel bir fark gözlenmedi (P>0.05). Bu sonuçlara göre, %9 G ilave edilen 7-DHCLC grubunun, diğer gruplara kıyasla eritme sonrası bazı spermat...

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Field and in vitro assay of three methods for freezing ram semen

Cited by 72 publications

References 23 publications

Adição de plasma seminal ao sêmen descongelado e taxa de prenhez de ovelhas inseminadas em tempo fixo

Adição de plasma seminal ao sêmen descongelado e taxa de prenhez de ovelhas inseminadas em tempo fixo

Effects of Bovine Serum Albumin and Trehalose in Semen Diluents for Improvement of Frozen-Thawed Ram Spermatozoa

Kolestrol ve 7-Dehidrokolestrolün Farkli Gliserol Oranlari İle Boğa Spermasi Dondurulmasina Etkisi

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