Field- and clinically derived estimates of
            <i>Wolbachia</i>
            -mediated blocking of dengue virus transmission potential in
            <i>Aedes aegypti</i>
            mosquitoes

Carrington, Lauren B.; Tran, Bich Chau Nguyen; Le, Nikitina; Luong, Tai Thi Hue; Nguyen, Truong An; Nguyen, Phong Thanh; Nguyen, Chau; Nguyen, Huong Thi Cam; Vu, Trung Tuan; Vo, Long Thi; Le, Dui Thi; Vu, Nhu Tuyet; Nguyen, Giang V.; Luu, Hung Quoc; Dang, Anh Duc; Hurst, Timothy P.; O’Neill, Scott L.; Tran, Vi; Kien, Duong Thi Hue; Nguyen, Nguyet Minh; Wolbers, Marcel; Wills, Bridget; Simmons, Cameron P.

doi:10.1073/pnas.1715788115

Cited by 105 publications

(109 citation statements)

References 41 publications

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“…Multiple traditional measures and strategies have been employed for dengue prevention and control, but most of these are compliance‐ and community involvement–dependent strategies which are hindered by poor compliance and absence of active local community involvement . Modern, integrated vector management approaches using novel biological control approaches such as paratransgenesis, sterile insect techniques, and production of genetically modified vectors, are urgently needed as well as innovative vaccine candidates. Our data presented in this study may be important for dengue vaccine development, particularly with regard to future vaccine composition.…”

Section: Discussionmentioning

confidence: 99%

Dengue viruses circulating in Indonesia: A systematic review and phylogenetic analysis of data from five decades

Harapan

Michie

Yohan

et al. 2019

Reviews in Medical Virology

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Although epidemiological and molecular epidemiological (serotype, genotype, and lineage information) data are available for several major cities in Indonesia, there is yet to be a comprehensive national study of dengue in Indonesia over time. This study was conducted to provide a comprehensive epidemiology of circulating dengue viruses (DENV) in Indonesia between 1973 and 2016. This was conducted through a systematic review of the literature and phylogenetic analysis of available DENV sequences. Available data from National Disease Surveillance System have indicated an increasing trend of dengue incidence in Indonesia over the past 50 years. Incidence rates appear to be cyclic, peaking approximately every 6 to 8 years. In contrast, the case fatality rate has decreased approximately by half with each decade since 1980.Over this 50-year time span, serotype shifts, genotype displacement within DENV-1 and DENV-2, and introduction of DENV-1 and DENV-3 genotype from other countries occurred. These events were associated with increased incidence of dengue cases. Our study also provides a valuable national snapshot of DENV genetic diversity in Indonesia that may contribute to development of more effective dengue vaccine compositions for the region.

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Section: Discussionmentioning

confidence: 99%

Dengue viruses circulating in Indonesia: A systematic review and phylogenetic analysis of data from five decades

Harapan

Michie

Yohan

et al. 2019

Reviews in Medical Virology

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“…For example, the infection of A. aegypti mosquitoes with selected strains of endosymbiotic Wolbachia resulted in bacterial invasion of mosquito populations and interference with DENV and ZIKV replication 282,283 . The wMel strain of Wolbachia-infected A. aegypti, when directly fed on viraemic dengue patients, has lower DENV transmission potential than their wild-type counterparts 284 . Mechanistic studies suggest that infection with Wolbachia reduces flavivirus replication, is associated with rapid viral RNA degradation in the cytoplasm and is mediated by the mosquito XRN1 enzyme 285 .…”

Section: Flavivirus Vaccinesmentioning

confidence: 98%

The continued threat of emerging flaviviruses

Pierson

Diamond²

2020

Nat Microbiol

595

627

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F laviviruses are single-stranded RNA viruses vectored principally by arthropods that cause severe illnesses in humans. The extensive global spread and epidemic transmission of flaviviruses during the last seven decades has been remarkable. The mosquito-borne dengue viruses (DENV) infect an estimated 400 million humans each year; more than a quarter of the world's population lives in areas where DENV is now endemic 1 . By comparison, only sporadic DENV epidemics were documented before the Second World War 2 . The introductions of West Nile (WNV) and Zika (ZIKV) viruses into the Western Hemisphere was followed by rapid geographical spread, large numbers of human infections and considerable morbidity 3,4 . Ongoing yellow fever virus (YFV) transmission and its encroachment on urban environments, despite the existence of an effective vaccine, poses a serious public health challenge 5-7 . Other flaviviruses present ongoing health risks or are beginning to emerge in different parts of the world, including Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBEV) and Usutu virus (USUV).The epidemic potential of flaviviruses reflects many factors related to the unique characteristics of their insect vectors, the consequences of poorly planned urbanization that creates ideal arthropod breeding habitats, the geographical expansion of vectors, changing environmental conditions and extensive global travel 8,9 . Beyond arthropods and humans, flaviviruses are also known to infect a wide array of animal species and can be important veterinary pathogens that threaten economically important domesticated animals 10-14 . These vertebrate animal hosts may constitute important stable reservoirs and contribute to defining conditions that support the introduction of new viral species and transmission among humans 15 . The continued threat of flavivirus emergence and re-emergence highlights a need for a detailed fundamental understanding of the biology of these viruses, the immune responses that can contain them and the possible countermeasures that can blunt their impact on public health should new outbreaks occur. Flavivirus structure and replicationFlaviviruses are small (~50 nm) spherical virus particles that incorporate a single genomic RNA of positive-sense polarity encoding three structural and seven non-structural proteins (Fig. 1a). Our knowledge of the biology of flaviviruses has advanced considerably with the availability of high-resolution structures of viral structural proteins and of virions at different stages of the replication cycle or in complex with antibodies or host factors 16 . Crystal structures of the enzymatic non-structural proteins also have been solved, accelerating advances in an understanding of virus replication and pathogenesis [17][18][19] and enabling structure-guided drug discovery, as reviewed elsewhere 20 .Virion structure and morphogenesis. Flaviviruses are assembled using three viral structural proteins (C, prM and E), a host lipid envelope and the viral genomic RNA. The structure of ...

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“…wMel has been well-characterised for its capacity to block DENV infection and replication in Ae. aegypti using different challenge methods [1,[13][14][15][16][17][18]. It demonstrates very effective virus blocking in mosquitoes that have been inoculated with virus, reducing the proportion of mosquitoes that become infected and reducing the tissue viral load (in a variety of tissues) by several orders of magnitude in those that do develop infection [15][16][17]19].…”

Section: Introductionmentioning

confidence: 99%

“…Viremic blood meals from acute dengue patients pose a more rigorous challenge to the virus blocking phenotype mediated by wMel, with some wMel-infected mosquitoes developing infectious saliva [14,15,18]. Nonetheless, this level of wMel-imparted anti-DENV resistance is projected to result in local elimination of DENV transmission in most endemic settings [14].…”

Section: Introductionmentioning

confidence: 99%

Multiple Wolbachia strains provide comparative levels of protection against dengue virus infection in Aedes aegypti

et al. 2020

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The insect bacterium Wolbachia pipientis is being introgressed into Aedes aegypti populations as an intervention against the transmission of medically important arboviruses. Here we compare Ae. aegypti mosquitoes infected with wMelCS or wAlbB to the widely used wMel Wolbachia strain on an Australian nuclear genetic background for their susceptibility to infection by dengue virus (DENV) genotypes spanning all four serotypes. All Wolbachiainfected mosquitoes were more resistant to intrathoracic DENV challenge than their wildtype counterparts. Blocking of DENV replication was greatest by wMelCS. Conversely, wAlbBinfected mosquitoes were more susceptible to whole body infection than wMel and wMelCS. We extended these findings via mosquito oral feeding experiments, using viremic blood from 36 acute, hospitalised dengue cases in Vietnam, additionally including wMel and wildtype mosquitoes on a Vietnamese nuclear genetic background. As above, wAlbB was less effective at blocking DENV replication in the abdomen compared to wMel and wMelCS. The transmission potential of all Wolbachia-infected mosquito lines (measured by the presence/ absence of infectious DENV in mosquito saliva) after 14 days, was significantly reduced compared to their wildtype counterparts, and lowest for wMelCS and wAlbB. These data support the use of wAlbB and wMelCS strains for introgression field trials and the biocontrol of DENV transmission. Furthermore, despite observing significant differences in transmission potential between wildtype mosquitoes from Australia and Vietnam, no difference was observed between wMel-infected mosquitoes from each background suggesting that Wolbachia may override any underlying variation in DENV transmission potential.

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Field- and clinically derived estimates of Wolbachia -mediated blocking of dengue virus transmission potential in Aedes aegypti mosquitoes

Cited by 105 publications

References 41 publications

Dengue viruses circulating in Indonesia: A systematic review and phylogenetic analysis of data from five decades

Dengue viruses circulating in Indonesia: A systematic review and phylogenetic analysis of data from five decades

The continued threat of emerging flaviviruses

Multiple Wolbachia strains provide comparative levels of protection against dengue virus infection in Aedes aegypti

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