Field and Clinical Applications of Advanced Bacteriophage-Based Detection of Yersinia pestis

Sergueev, Kirill V.; Nikolich, Mikeljon P.; Filippov, Andrey A.

doi:10.1007/978-1-4614-3561-7_17

Cited by 7 publications

(5 citation statements)

References 19 publications

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“…Carrillo et al demonstrated Campylobacter isolates which significantly reduced the ability to colonize the broiler intestine compared with the original susceptible strain [54]. Further, Filippov et al proved that the phage-resistant Yersinia pestis was less virulent because of modifications in lipopolisacharides (LPS), and Lin et al showed similar effects in Klebsiella pneumoniae when the K1 capsule, being a phage receptor, was modified [55,56]. Moreover, there was a study in which a phage-resistant V. anguillarum strain lost its virulence, which was notified by the reduced mortality of cod [57].…”

Section: Bacterial Resistance and Other Safety Aspectsmentioning

confidence: 99%

Growing Trend of Fighting Infections in Aquaculture Environment—Opportunities and Challenges of Phage Therapy

Kowalska¹,

Kazimierczak²,

Sowińska³

et al. 2020

Antibiotics

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Phage therapy, a promising alternative to antimicrobial treatment of bacterial diseases, is getting more and more popular, especially due to the rising awareness of antibiotic resistance and restrictions in antibiotics’ use. During recent years, we observed a growing trend of bacteriophages’ application in aquaculture, which in each year reports high losses due to bacterial diseases. This review provides an update of the status of bacteriophage therapy for the treatment and prevention of infections in the aquatic environment. As it is still mostly in the scientific stage, there are a few constraints that may prevent effective therapy. Therefore, specific characteristics of bacteriophages, that can act in favor or against their successful use in treatment, were described. We underlined aspects that need to be considered: specificity of phages, bacterial resistance, safety, immune response of the host organism, formulation, administration and stability of phage preparations as well as bacteriophages’ influence on the environment. The biggest challenge to overcome is finding the right balance between the desired and problematic characteristics of bacteriophages. Finally, regulatory approval challenges may be encountered by bacteriophage manufacturers. Even though there are still some technical constraints connected with the global use of bacteriophage therapy, it was concluded that it can be successfully applied in aquaculture.

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Section: Bacterial Resistance and Other Safety Aspectsmentioning

confidence: 99%

Growing Trend of Fighting Infections in Aquaculture Environment—Opportunities and Challenges of Phage Therapy

Kowalska¹,

Kazimierczak²,

Sowińska³

et al. 2020

Antibiotics

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“…ϕA1122 is able to infect most Y. pestis strains tested and will only infect the near neighbor Y. pseudotuberculosis under certain temperature constraints [24,55,63]. Two other phages are used for plague diagnosis, the Pokrovskaya phage and the L-413C phage [25,26].…”

Section: Plaguementioning

confidence: 99%

“…developed a quantitative real-time PCR (real time-qPCR) assay for ϕA1122 and L-413C that could be used to detect replicating phage DNA in live Y. pestis with a sensitivity of 10 3 cfu/mL to 10 5 cfu/mL or one bacterium to 100 cfu per qPCR sample, respectively [25]. This assay proved to be suitable for both lab-based (LightCycler 2.0) and field-deployable (JBAIDS) real time-qPCR systems [26]. The assay was sensitive in simulated blood specimens, serum, and organ samples.…”

Section: Plaguementioning

confidence: 99%

The State of the Art in Biodefense Related Bacterial Pathogen Detection Using Bacteriophages: How It Started and How It’s Going

Sozhamannan

Hofmann

2020

Viruses

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Accurate pathogen detection and diagnosis is paramount in clinical success of treating patients. There are two general paradigms in pathogen detection: molecular and immuno-based, and phage-based detection is a third emerging paradigm due to its sensitivity and selectivity. Molecular detection methods look for genetic material specific for a given pathogen in a sample usually by polymerase chain reaction (PCR). Immuno-methods look at the pathogen components (antigens) by antibodies raised against that pathogen specific antigens. There are different variations and products based on these two paradigms with advantages and disadvantages. The third paradigm at least for bacterial pathogen detection entails bacteriophages specific for a given bacterium. Sensitivity and specificity are the two key parameters in any pathogen detection system. By their very nature, bacteriophages afford the best sensitivity for bacterial detection. Bacteria and bacteriophages form the predator-prey pair in the evolutionary arms race and has coevolved over time to acquire the exquisite specificity of the pair, in some instances at the strain level. This specificity has been exploited for diagnostic purposes of various pathogens of concern in clinical and other settings. Many recent reviews focus on phage-based detection and sensor technologies. In this review, we focus on a very special group of pathogens that are of concern in biodefense because of their potential misuse in bioterrorism and their extremely virulent nature and as such fall under the Centers for Disease and Prevention (CDC) Category A pathogen list. We describe the currently available phage methods that are based on the usual modalities of detection from culture, to molecular and immuno- and fluorescent methods. We further highlight the gaps and the needs for more modern technologies and sensors drawing from technologies existing for detection and surveillance of other pathogens of clinical relevance.

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“…As mentioned, due to the limitations of traditional antibodies, a number of alternative amino acid-based recognition elements have been explored that remove the immunoglobulin scaffold. One such alternative with growing interest is phage display where recent efforts have been focused on transitioning to clinical detection. , Phage display offers an alternative backbone by using the bacteriophage particle for selection while providing a variable region for binding similar to that of antibodies. Phage have an immense sequence pool, and are estimated to be the most abundant organism on the planet numbering roughly 10 31 ! Use of this format also provides the ability to potentially differentiate between live and dead cells with bacteriophage replication assays, which require a viable host bacteria for growth; this is a unique diagnostic tool not achievable using DNA hybridization assays .…”

Section: Biochemical Sensorsmentioning

confidence: 99%

“…One such alternative with growing interest is phage display where recent efforts have been focused on transitioning to clinical detection. 331,332 Phage display offers an alternative backbone by using the bacteriophage particle for selection while providing a variable region for binding similar to that of antibodies. Phage have an immense sequence pool, and are estimated to be the most abundant organism on the planet numbering roughly 10 31 !…”

Section: ■ Biochemical Sensorsmentioning

confidence: 99%

Detecting Biothreat Agents: From Current Diagnostics to Developing Sensor Technologies

et al. 2018

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Although a fundamental understanding of the pathogenicity of most biothreat agents has been elucidated and available treatments have increased substantially over the past decades, they still represent a significant public health threat in this age of (bio)terrorism, indiscriminate warfare, pollution, climate change, unchecked population growth, and globalization. The key step to almost all prevention, protection, prophylaxis, post-exposure treatment, and mitigation of any bioagent is early detection. Here, we review available methods for detecting bioagents including pathogenic bacteria and viruses along with their toxins. An introduction placing this subject in the historical context of previous naturally occurring outbreaks and efforts to weaponize selected agents is first provided along with definitions and relevant considerations. An overview of the detection technologies that find use in this endeavor along with how they provide data or transduce signal within a sensing configuration follows. Current "gold" standards for biothreat detection/diagnostics along with a listing of relevant FDA approved in vitro diagnostic devices is then discussed to provide an overview of the current state of the art. Given the 2014 outbreak of Ebola virus in Western Africa and the recent 2016 spread of Zika virus in the Americas, discussion of what constitutes a public health emergency and how new in vitro diagnostic devices are authorized for emergency use in the U.S. are also included. The majority of the Review is then subdivided around the sensing of bacterial, viral, and toxin biothreats with each including an overview of the major agents in that class, a detailed cross-section of different sensing methods in development based on assay format or analytical technique, and some discussion of related microfluidic lab-on-a-chip/point-of-care devices. Finally, an outlook is given on how this field will develop from the perspective of the biosensing technology itself and the new emerging threats they may face.

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Field and Clinical Applications of Advanced Bacteriophage-Based Detection of Yersinia pestis

Cited by 7 publications

References 19 publications

Growing Trend of Fighting Infections in Aquaculture Environment—Opportunities and Challenges of Phage Therapy

Growing Trend of Fighting Infections in Aquaculture Environment—Opportunities and Challenges of Phage Therapy

The State of the Art in Biodefense Related Bacterial Pathogen Detection Using Bacteriophages: How It Started and How It’s Going

Detecting Biothreat Agents: From Current Diagnostics to Developing Sensor Technologies

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