Fibrous Tissue of Subchondral Cystic Lesions in Horses Produce Local Mediators and Neutral Metalloproteinases and Cause Bone Resorption in Vitro

Rechenberg, Brigitte von; Guenther, Harald L.; McIlwraith, C. Wayne; Leutenegger, Christian M.; Frisbie, D. D.; Akens, Margarete K.; Auer, Jörg A

doi:10.1053/jvet.2000.7538

Cited by 86 publications

(79 citation statements)

References 32 publications

(76 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The heterogenicity of the selection of joints and their stage of disease where the samples were taken from was not optimal in this study; however, later similar investigations in equine synovial membrane and articular caftilage, where all tissues were taken from the same joints and where differences were made between stage of disease, confirmed the results of this study in all points (Rechenberg et al, 1999a;Rechenberg et al,, 1 999b) Comparing the groups, the wet weight of tissue/ml of medium was judged to be a factor to consider. An amount of 25 mg wet weigh /ml of medium seemed desirable to obtain reliable results, at least for measuring NO concentrations in articular cartilage explants.…”

Section: Discussionmentioning

confidence: 47%

“…lt was felt, that scoring of joint disease was not necessary for the goals of this study. This was supported by separate investigations by the same authors in horses (Rechenberg et al, 1999a), dogsl and sheep (Akens et al, 1998), Development of NO and PGE2 over time in culture was not influenced by the stage of disease of the tissues where samples were taken from. lt was hoped that this study would serve as a basis for future investigations of the spontaneous production of other important substances involved in articular caftilage degradation in an in vitro explant culture system.…”

Section: Pferdeheilkunde 15mentioning

confidence: 57%

See 1 more Smart Citation

Temporal production of nitric oxide and Prostaglandin E2 in media of explant cultures of equine synovial membrane and articular cartilage

Rechenberg¹,

McIllwraith²,

Akens³

et al. 1999

PHK

View full text Add to dashboard Cite

SummaryThe local mediators nitric oxide (NO) and prostaglandin E, (PGE ) play a role in the cascade of extracellular matrix breakdown in articular cartilage. In the present study the spontaneous production of NO and PGE, in explant cultures of equine synovial membrane and articular cartilage and their concentration over tjme n culture was investigated and correlated. Synovial membrane and articular cartilage was harvested from metacarpophalangeal jonts of 33 horses durlng arthroscopic surgery (n=4) or after being slaughtered/euthanised for reasons other than infectious or metabolic dlsease (n=29). Trssues were cultured as explant cuJtures in the presence of 4 ml Ham's F 12 medium, supplemented with streptomycin (100 pg/ml) and penicillin (100 U/ml) and incubated in a 5% CO2 and water saturated atmosphere for 8 days. Re sults showed that generally equine synovial membrane produced less NO than articular cartilage explant cultures with comparable wet weights/ml of media, but significantly more PGE, (P<0.05). The concentrations of both mediators were relatively stable over the first 4 days of culture, at least in the group of 25 mg wet wdghvml of media. In this group, there was a weak tendency noticed for NO to decrease, and PGE, to increase over time with day 4 being the intersection for both. Tissue cultures of articular cartilage with 12.5 mg wet weight/ml of culture media produced less reliable results, especially with NO measurements, which was thought to be associated with the sensitivity of the assay. lt is recommended to collect conditioned media for the measurements of NO and PGE, within the first 4 days of explant culture, since atter this time period culture effects may mask the results at least for NO measurements. Both mediators may be factors that play an impor tant role in the local med ation of ioint inflammations. Keywords:nitric oxide, prostaglandin Er, synovial membrane, articular cartilage, equine Die zeitlich bedingte Produktion von Stickoxid (NO) und Prostaglandin E, (PGEr) in Medium von Gewebekultur€n aus Synovialmembran und GelenkknorDel von PferdenDie lokalen Mediatoren, Stickoxid (nitric oxide = NO) und Prostaglandin E, (PGEr) spielen eine Rolle in der Kaskade des Abbaus der extrazellulären lvlatrix von Knorpel und Knochen. In der vorliegenden Studie wurde die spontane Produktion und Konzentration von NO und PGE2 in Gewebekulturen aus Synovialmembran und Gelenkknorpel von Pferden über eine Zeit von B Tagen untersucht und korreliert. Bei 33 Pferden wurde während der Arthroskopie des Gelenkes {n=4) oder zum Zeitpunkt der Schlachtung (n=29) Synovialmembran und Gelenkknorpel aus dem Fesselgelenk entnommen. Das Gewebe wurde in der Gewebekultur ('12.5 mg/ml Medium und 25 mg/ml Medium) n Ham's F-1 2 Medi um, angereichert mit Streptomycin (100 pg/ml) und Penicillin (100 U/ml), unter Standardbedingungen von 5% CO, und mit Wasser gesättig-ter Atmosphäre für B Tage inkubiert. Es wurde gezeigt, dass allgemein die Synovialmembran Gewebekulturen deutlich weniger NO produzierten als die Gelenkknorpel Gew...

show abstract

Section: Discussionmentioning

confidence: 47%

Section: Pferdeheilkunde 15mentioning

confidence: 57%

Temporal production of nitric oxide and Prostaglandin E2 in media of explant cultures of equine synovial membrane and articular cartilage

Rechenberg¹,

McIllwraith²,

Akens³

et al. 1999

PHK

View full text Add to dashboard Cite

show abstract

“…In addition, in vitro studies have shown that fibrous tissue from equine bone cysts can produce enzymes and cytokines that are associated with bone resorption. 49,50 Both microfractures and enzyme production may result in progressive enlargement of the lesion until it can no longer support the overlying articular cartilage and gives way to infolding. The small cartilage folds superficial to the current lesions I-J and L confirm that cartilage will withstand some deformation 25 but may eventually break because the interior of lesions M-O communicated with the joint space by way of cloacae running through the large cartilage folds superficial to each of these lesions.…”

Section: True Cystsmentioning

confidence: 99%

Osteochondrosis Can Lead to Formation of Pseudocysts and True Cysts in the Subchondral Bone of Horses

et al. 2014

View full text Add to dashboard Cite

Osteochondrosis arises as a result of focal failure of the blood supply to growth cartilage. The current aim was to examine the pathogenesis of pseudocysts and true cysts in subchondral bone following failure of the blood supply to the articular-epiphyseal cartilage complex in horses. Cases were recruited based on identification of lesions (n ¼ 17) that were considered likely to progress to or to represent pseudocysts or true cysts in epiphyseal bone in histological sections and included 10 horses ranging in age from 48 days to 5 years old. Cases comprised 3 warmbloods, 3 Standardbreds, 1 Quarter horse and 1 Arabian with spontaneous lesions and 2 Fjord ponies with experimentally induced lesions. Seven lesions consisted of areas of ischemic chondronecrosis and were compatible with pseudocysts. Two lesions were located at intermediate depth in epiphyseal growth cartilage, 2 lesions were located in the ossification front, 2 lesions were located in epiphyseal bone and 1 lesion was located in the metaphyseal growth plate (physis). Ten lesions contained dilated blood vessels and were compatible with true cysts. In 2 lesions the dilated blood vessels were located within the lumina of failed cartilage canals. In the 8 remaining lesions areas of ischemic chondronecrosis were associated with granulation tissue in the subjacent bone and dilated vessels were located within this granulation tissue. Failure of the blood supply and ischemic chondronecrosis can lead to formation of pseudocysts or dilatation of blood vessels and formation of true cysts in the epiphyseal bone of horses.

show abstract

“…In addition, conditioned media of SCL tissue was capable of recruiting osteoclasts and increasing their activity (von Rechenberg et al 2000). Further work with in situ hybridization of sections of fibrous tissue of SCLs, as well as quantitative PCR with fresh frozen fibrous tissue and undecalcified sections of SCLs showed upregulation of mRNA of interleukin-1 (IL-1) and interleukin-6 (IL-6) in the fibrous tissue of SCLs (von Rechenberg et al 2001).…”

Section: Pathogenesismentioning

confidence: 99%

Die Behandlung suchchondraler Knochenzysten des medialen Femurkondylus - Fortschritte auf der Basis von Forschung und klinischer Erfahrung

McIllwraith¹

2010

PHK

View full text Add to dashboard Cite

Fibrous Tissue of Subchondral Cystic Lesions in Horses Produce Local Mediators and Neutral Metalloproteinases and Cause Bone Resorption in Vitro

Abstract: Understanding the pathogenesis of SCL will help to establish successful therapy in horses affected with SCL.

Cited by 86 publications

References 32 publications

Temporal production of nitric oxide and Prostaglandin E2 in media of explant cultures of equine synovial membrane and articular cartilage

Temporal production of nitric oxide and Prostaglandin E2 in media of explant cultures of equine synovial membrane and articular cartilage

Osteochondrosis Can Lead to Formation of Pseudocysts and True Cysts in the Subchondral Bone of Horses

Die Behandlung suchchondraler Knochenzysten des medialen Femurkondylus - Fortschritte auf der Basis von Forschung und klinischer Erfahrung

Contact Info

Product

Resources

About