Fibrose superficielle radio-induite et TGF-β1

Martin, Michèle; Delanian, S.; Sivan, Virginie; Vozenin, Marie-Catherine; Reisdorf, Philippe; Lawrence, David A.; Lefaix, Jean-Louis

doi:10.1016/s1278-3218(00)00010-x

Cited by 20 publications

(5 citation statements)

References 118 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similarly to other works [14], [30], [31], [32], particularly in the skin, a high level of TGF-β1 was found throughout the study. TGF-β1 is known to control the regulation and inhibition of cell growth and the homeostasis of the extracellular matrix.…”

Section: Discussionsupporting

confidence: 87%

See 1 more Smart Citation

Long-Term Alterations of Cytokines and Growth Factors Expression in Irradiated Tissues and Relation with Histological Severity Scoring

Gallet¹,

Phulpin

Merlin

et al. 2011

PLoS ONE

View full text Add to dashboard Cite

PurposeBeside its efficacy in cancer treatment, radiotherapy induces degeneration of healthy tissues within the irradiated area. The aim of this study was to analyze the variations of proinflammatory (IL-1α, IL-2, IL-6, TNF-α, IFN-γ), profibrotic (TGF-β1), proangiogneic (VEGF) and stem cell mobilizing (GM-CSF) cytokines and growth factors in an animal model of radiation-induced tissue degeneration.Materials and Methods24 rats were irradiated unilaterally on the hindlimb at a monodose of 30 Gy. Six weeks (n = 8), 6 months (n = 8) and 1 year (n = 8) after irradiation the mediators expression in skin and muscle were analyzed using Western blot and the Bio-Plex® protein array (BPA) technology. Additional histological severity for fibrosis, inflammation, vascularity and cellularity alterations scoring was defined from histology and immnunohistochemistry analyses.ResultsA significant increase of histological severity scoring was found in irradiated tissue. Skin tissues were more radio-sensitive than muscle. A high level of TGF-β1 expression was found throughout the study and a significant relation was evidenced between TGF-β1 expression and fibrosis scoring. Irradiated tissue showed a chronic inflammation (IL-2 and TNF-α significantly increased). Moreover a persistent expression of GM-CSF and VEGF was found in all irradiated tissues. The vascular score was related to TGF-β1 expression and the cellular alterations score was significantly related with the level of IL-2, VEGF and GM-CSF.ConclusionThe results achieved in the present study underline the complexity and multiplicity of radio-induced alterations of cytokine network. It offers many perspectives of development, for the comprehension of the mechanisms of late injuries or for the histological and molecular evaluation of the mode of action and the efficacy of rehabilitation techniques.

show abstract

Section: Discussionsupporting

confidence: 87%

“…Histologically, the four main phenomena involved in late effects development appear to be inflammation, fibrosis, vascular alterations and cellular depletion [3], [4], [5], [6]. The involvement of each of these phenomena in the genesis of late effects is highly debated.…”

Section: Introductionmentioning

confidence: 99%

Long-Term Alterations of Cytokines and Growth Factors Expression in Irradiated Tissues and Relation with Histological Severity Scoring

Gallet¹,

Phulpin

Merlin

et al. 2011

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Previous studies also demonstrated that TGF-β1 plays an important role in radiation-induced development of fibrosis. 16 , 17 Together with these findings, we believe that increased TGF-β1 postradiation might be closely related to radiation-induced fibrosis. We further found that the expression of TGF-β1 mRNA was significantly decreased in the IFN-γ group compared to that in the conventional radiation group, suggesting that IFN-γ inhibits radiation-induced increases in TGF-β1 mRNA in rectal and ureteral tissues; however, the expression of TGF-β1 mRNA in the IFN-γ group was still higher than that in the control group, suggesting that IFN-γ cannot completely inhibit radiotherapy-induced upregulation of TGF-β1 mRNA.…”

Section: Discussionsupporting

confidence: 58%

The effect of interferon gamma on conventional fractionated radiation-induced damage and fibrosis in the pelvic tissue of rabbits

Yang

Wang

et al. 2016

TCRM

View full text Add to dashboard Cite

We aim to investigate the effect of interferon gamma (IFN-γ) on conventional fractionated radiation–induced damage and fibrosis in ureter and colorectal mucosa. Fifty-two rabbits were randomly divided into three groups comprising a conventional radiation group, an IFN-γ group, and a control group. X-rays were used to irradiate the pelvic tissues of the rabbits in the IFN-γ and conventional radiation groups. Five days after radiation exposure, the rabbits in the IFN-γ group were administered 250,000 U/kg IFN-γ intramuscularly once a week for 5 weeks. The rabbits in the conventional radiation group received 5.0 mL/kg saline. The rabbits were sacrificed at 4, 8, 12, and 16 weeks postradiation, and the rectal and ureteral tissues within the radiation areas were collected. The results showed that the morphology of rectal and ureteral tissues was changed by X-ray radiation. The degree of damage at 4, 8, and 12 weeks, but not at 16 weeks, postradiation was significantly different between the IFN-γ and conventional radiation groups. The expression of transforming growth factor beta 1 mRNA in the ureter and colorectal mucosa of the IFN-γ group was significantly lower than that in the conventional radiation group at 4, 8, 12, and 16 weeks postradiation, but it was still higher than that in the control group. There were significant differences in the expression of collagen III among the three groups. IFN-γ can inhibit the radiation-induced upregulation of transforming growth factor beta 1 mRNA and collagen III protein in the ureter and colorectal mucosa and attenuate radiation-induced damage and fibrosis.

show abstract

“…Radiation pneumonitis is one of the most frequent post-treatment complications of stereotactic body radiotherapy (SBRT), with symptoms ranging from mild to critical. In particular, radiation-induced lung fibrosis (RILF), which is characterized by the recruitment of fibroblasts, myofibroblasts, and leukocytes, resulting in the accumulation of extracellular matrix proteins, such as collagens, is a severe complication that may be life-threatening due to the impairment of normal breathing [ 1 – 3 ]. At present, steroid anti-inflammatory drugs are the only medical option for patients with a high risk of RILF onset prior to augmentation of the pathogenic response.…”

Section: Introductionmentioning

confidence: 99%

Induction of MiR-21 by Stereotactic Body Radiotherapy Contributes to the Pulmonary Fibrotic Response

et al. 2016

View full text Add to dashboard Cite

Radiation-induced lung fibrosis, the most serious effect of lung cancer radiotherapy on normal tissue, remains a major technical obstacle to the broader application of radiotherapy to patients with lung cancer. This study describes the use of an image-guided irradiation system in mice mimicking stereotactic body radiotherapy (SBRT) to examine the molecular features of chronic fibrotic response after radiation injury. MicroRNA (miR) array analysis of injured pulmonary tissue identified a set of miRs whose expression was significantly increased in damaged lung tissue. In particular, miR-21 expression was increased at the radiation injury site, concurrent with collagen deposition. Although the inhibition of miR-21 by its specific inhibitor anti-miR-21 only marginally affected endothelial-mesenchymal transition (EndMT) in lung endothelial cells, this inhibition significantly reduced collagen synthesis in lung fibroblasts. Furthermore, ectopic expression of miR-21 was sufficient to promote a fibrotic response in lung fibroblasts, enhancing Smad2 phosphorylation concurrent with Smad7 downregulation. These findings indicate that the induction of miR-21 expression is responsible for fibrotic responses observed in mesenchymal cells at the injury site through the potentiation of TGF-β signaling. Local targeting of miR-21 at the injured area could have potential therapeutic utility in mitigating radiation-induced lung fibrosis.

show abstract

Fibrose superficielle radio-induite et TGF-β1

Cited by 20 publications

References 118 publications

Long-Term Alterations of Cytokines and Growth Factors Expression in Irradiated Tissues and Relation with Histological Severity Scoring

Long-Term Alterations of Cytokines and Growth Factors Expression in Irradiated Tissues and Relation with Histological Severity Scoring

The effect of interferon gamma on conventional fractionated radiation-induced damage and fibrosis in the pelvic tissue of rabbits

Induction of MiR-21 by Stereotactic Body Radiotherapy Contributes to the Pulmonary Fibrotic Response

Contact Info

Product

Resources

About