Fibronectin regulates the self-renewal of rabbit limbal epithelial stem cells by stimulating the Wnt11/Fzd7/ROCK non-canonical Wnt pathway

Zheng, Mingyue; Tian, Chenglei; Fan, Ting-Jun; Xu, Bo

doi:10.1016/j.exer.2019.05.021

Cited by 14 publications

(16 citation statements)

References 66 publications

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“…An important unsolved question is the adequate characterization of the xenograft that allow us to definitely identify the origin of the cells found in the rabbit cornea. Rabbit limbal epithelial stem cells show rapid self-renewal (Zheng et al, 2019), and grafted human cells may disappear after several months of in vivo follow-up. In this regard, we found that two primary antibodies (anti-PKG and anti-CRY-λ1) showed positive expression in corneas grafted in rabbits, and very low expression in rabbit controls.…”

Section: Discussionmentioning

confidence: 99%

Long-Term in vivo Evaluation of Orthotypical and Heterotypical Bioengineered Human Corneas

Garzón

Chato‐Astrain

González-Gallardo

et al. 2020

Front. Bioeng. Biotechnol.

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Purpose: Human cornea substitutes generated by tissue engineering currently require limbal stem cells for the generation of orthotypical epithelial cell cultures. We recently reported that bioengineered corneas can be fabricated in vitro from a heterotypical source obtained from Wharton's jelly in the human umbilical cord (HWJSC). Methods: Here, we generated a partial thickness cornea model based on plastic compression nanostructured fibrin-agarose biomaterials with cornea epithelial cells on top, as an orthotypical model (HOC), or with HWJSC, as a heterotypical model (HHC), and determined their potential in vivo usefulness by implantation in an animal model. Results: No major side effects were seen 3 and 12 months after implantation of either bioengineered partial cornea model in rabbit corneas. Clinical results determined by slit lamp and optical coherence tomography were positive after 12 months. Histological and immunohistochemical findings demonstrated that in vitro HOC and HHC had moderate levels of stromal and epithelial cell marker expression, whereas in vivo grafted corneas were more similar to control corneas. Conclusion: These results suggest that both models are potentially useful to treat diseases requiring anterior cornea replacement, and that HHC may be an efficient alternative to the use of HOC which circumvents the need to generate cornea epithelial cell cultures.

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Section: Discussionmentioning

confidence: 99%

Long-Term in vivo Evaluation of Orthotypical and Heterotypical Bioengineered Human Corneas

Garzón

Chato‐Astrain

González-Gallardo

et al. 2020

Front. Bioeng. Biotechnol.

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“…The ECM of the limbal niche is a dynamic microenvironment that provides mechanical and structural support for stem cells but also regulates cellular functions. It has been shown that fibronectin enhances proliferation and regulates stemness of rabbit limbal epithelial stem cells [ 61 ]. In addition, during wound healing, fibronectin/fibrinogen receptors are up-regulated on epithelial cells, which migrate over the bare wound [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

Expansion of Human Limbal Epithelial Stem/Progenitor Cells Using Different Human Sera: A Multivariate Statistical Analysis

Hernáez‐Moya

González

Urkaregi

et al. 2020

IJMS

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Transplantation of human cultured limbal epithelial stem/progenitor cells (LESCs) has demonstrated to restore the integrity and functionality of the corneal surface in about 76% of patients with limbal stem cell deficiency. However, there are different protocols for the expansion of LESCs, and many of them use xenogeneic products, being a risk for the patients’ health. We compared the culture of limbal explants on the denuded amniotic membrane in the culture medium—supplemental hormone epithelial medium (SHEM)—supplemented with FBS or two differently produced human sera. Cell morphology, cell size, cell growth rate, and the expression level of differentiation and putative stem cell markers were examined. Several bioactive molecules were quantified in the human sera. In a novel approach, we performed a multivariate statistical analysis of data to investigate the culture factors, such as differently expressed molecules of human sera that specifically influence the cell phenotype. Our results showed that limbal cells cultured with human sera grew faster and contained similar amounts of small-sized cells, higher expression of the protein p63α, and lower of cytokeratin K12 than FBS cultures, thus, maintaining the stem/progenitor phenotype of LESCs. Furthermore, the multivariate analysis provided much data to better understand the obtaining of different cell phenotypes as a consequence of the use of different culture methodologies or different culture components.

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“…The extracellular matrix (ECM) is a dynamic microenvironment whose components provide the structural support to the cells, in addition to the regulatory effects in term of controlling cellular signaling, shape, migration, proliferation, and differentiation (Dominici et al, ; Hibi, Yamada, Ueda, & Endo, ; Zheng, Tian, Fan, & Xu, ). The main task for the scaffold in BTE is to create an environment that provides the cells with mechanical support required for 3D proliferation and growth.…”

Section: Introductionmentioning

confidence: 99%

“…COL1 and FN are ECM proteins that contribute to cell activity and involve in regulating DPSCs. They play crucial roles in adhesion, migration, and osteogenic differentiation of stem cells (Alanazi et al, ; Kang et al, ; Mittag et al, ; Zheng et al, ). COL1 as collagen sponges is a natural material has the advantages of its biocompatibility, abundance, and its highly porous.…”

Section: Introductionmentioning

confidence: 99%

“…This might be used as a substrate to modify scaffold materials used in BTE. It has been reported that FN stimulates the expansion and osteogenic differentiation of stem cells (Andreeva, Leonova, Popenko, & Belyavsky, ; Kang et al, ; Zheng et al, ), but no previous experiments have investigated the effect of COL1 and FN coating on osteogenic differentiation of dental pulp cells (DPCs). This in vitro study was aimed to investigate the influence of COL1 and FN coating on attachment and proliferation of hDPCs and the effect of COL1 and FN on osteogenic differentiation of DPCs at gene and protein levels.…”

Section: Introductionmentioning

confidence: 99%

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Biological responses of dental pulp cells to surfaces modified by collagen 1 and fibronectin

Omer

Al-Sharabi

Qiu

et al. 2020

J Biomedical Materials Res

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Collagen 1 (COL1) and fibronectin (FN) are extracellular matrix proteins that contribute in cell activity and involve in regulating dental pulp cells (DPCs). The purpose of this study was to investigate the effect of COL1 and FN on the behavior of DPCs. Here, DPCs were grown under three different conditions: COL1 coating, FN coating, and control group without coating. The proliferation and differentiation of DPCs were investigated. DPCs in osteogenic media were able to differentiate into osteoblastic phenotype. The morphological analysis revealed no obvious difference on the shape of cells. Cells had spread well on both coated and noncoated culture plates with slightly more spreading in the coated plates after 24 hr. The MTT analysis did not demonstrate a significant difference at 1 and 3 hr among the groups, but interestingly, the analysis disclosed more cells on the coated plates after longer cultures, which indicated a higher proliferative capacity in response to COL1 and FN. RT‐PCR, Western Blotting and mineralization assays did not reveal significant differences between the coated and noncoated surfaces in relation to osteogenic differential potential. Our data suggested that the surface coating of COL1 and FN were able to promote cellular proliferation and the osteogenic differentiation tendency of DPCs was also observed in vitro.

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Fibronectin regulates the self-renewal of rabbit limbal epithelial stem cells by stimulating the Wnt11/Fzd7/ROCK non-canonical Wnt pathway

Cited by 14 publications

References 66 publications

Long-Term in vivo Evaluation of Orthotypical and Heterotypical Bioengineered Human Corneas

Long-Term in vivo Evaluation of Orthotypical and Heterotypical Bioengineered Human Corneas

Expansion of Human Limbal Epithelial Stem/Progenitor Cells Using Different Human Sera: A Multivariate Statistical Analysis

Biological responses of dental pulp cells to surfaces modified by collagen 1 and fibronectin

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