Fibronectin Promotes Cell Cycle Entry in Smooth Muscle Cells in Primary Culture

Curcumin, a nutritional supplement with antineoplastic activity, enhances leiomyoma cell apoptosis and decreases fibronectin expression

“…In further support of the role fibronectin plays in the interrelationship between the intracellular structure and ECM, disruption of cytoskeletal actin also disrupts fibronectin matrix organization (71) while fibronectin can regulate smooth muscle cell entry into the cell cycle (72). Furthermore, fibronectin expression requires activation of NF-kB and AP-1, both of which can be induced by stress (73).…”

Allogenic immune response promotes the accumulation of host-derived smooth muscle cells in transplant arteriosclerosis

“…The common carotid artery of female LEW or F344 rats anesthesized with Hypnonorm/Dormicum was injured with a Fogarty 2-F balloon catheter inserted through the external carotid artery [15]. The common carotid artery was temporarily occluded proximally and distally by ligatures.…”

“…SMCs were isolated from the aortic media of male F344 rats by collagenase digestion and cultured in medium F-12 supplemented with 50 Ag/ml l-ascorbic acid, 50 Ag/ml streptomycin, 50 IU/ml penicillin, and 10% fetal calf serum (F-12/10% FCS; GIBCO, Rockville, MD) as described [15]. The cultures were grown to confluency and trypsinized, and secondary cultures were established in F-12/10% FCS.…”

Angiogenic factors FGF2 and PDGF-BB synergistically promote murine tumor neovascularization and metastasis

“…VSMCs were isolated from the aortic media of male F344 rats by collagenase digestion and cultured in F-12 Ham medium (GIBCO) supplemented with 50 μg/ml L-ascorbic acid, 50 μg/ml streptomycin, 50 IU/ml penicillin, and 20% FCS (GIBCO) as previously described (40). These cells were kindly provided by Eric Wahlberg's laboratory at the Karolinska Hospital, Stockholm, Sweden.…”