Fibronectin localization in the mouse embryo from the two cell stage to the morula stage.

Fibronectin localization in the mouse embryo from the blastocyst stage to the egg cylinder stage in vitro.

“…Using the direct and indirect immunofluorescence techniques, we found fibronectin on the blastocyst, and the trophoblast cells of the egg cylinder stage embryo, especially at the sites of cell spreading, as well as the inner cell mass. The results show that (i) fibronectin is used for the initial cell attachment to the plastic dish, and (ii) during the course of embryo growth in vitro, the trophoblast cells spread over the plastic dish in the area of cells which contain many granules of fibronectin.fibronectin ; mouse embryo ; ovum implantation ; trophoblast ; immunofluorescent antibody technique Among the characteristics of fibronectin, the adhesiveness and the cell migration promotion may facilitate the embryo implantation.Although it was reported that fibronectin did not appear in the mouse embryo until the late blastocyst stage, (Zetter and Martin 1978; Wartiovaara et al 1979), we found fibronectin in the mouse embryo from the two cell stage (Yohkaichiya et al 1988), and our findings indicate that fibronectin is not abruptly produced just before the embryo implantation, but it is gradually produced along the course of embryo growth at least from the two cell stage. In order to investigate the role of fibronectin at the time of embryo implantation the techniques of direct and indirect immunofluorescence were used to locate the fibronectin on the delicately fixed mouse blastocysts and the egg cylinder stage embryos which were cultured in vitro from the blastocyst stage.…”

“…Although it was reported that fibronectin did not appear in the mouse embryo until the late blastocyst stage, (Zetter and Martin 1978;Wartiovaara et al 1979), we found fibronectin in the mouse embryo from the two cell stage (Yohkaichiya et al 1988), and our findings indicate that fibronectin is not abruptly produced just before the embryo implantation, but it is gradually produced along the course of embryo growth at least from the two cell stage. In order to investigate the role of fibronectin at the time of embryo implantation the techniques of direct and indirect immunofluorescence were used to locate the fibronectin on the delicately fixed mouse blastocysts and the egg cylinder stage embryos which were cultured in vitro from the blastocyst stage.…”

“…4) indicate that at least in the initial phase of in vitro embryo attachment the embryo does not require exogenous fibronectin. The reason may be explained by our previous reports (Yohkaichiya et al 1988) that the embryo itself produces fibronectin on its surface before hatching.…”

Restricted distribution of integrin beta 6 mRNA in primate epithelial tissues.

“…It is also possible that a v integrins play a role in preparing the endometrium for implantation of the embryo, which takes place in mid-secretory phase at a time when av integrin expression is up-regulated. Since fibronectin is present on the surface of the blastocyst (Turpeenniemi-Hujanen et al, 1992;Yohkaichiya et al, 1988) it is conceivable that avP6 expressed by the endometrium could be involved in the initial attachment of the embryo to the uterine surface epithelium. Studies on the subcellular distribution of p6 protein and its ligand-binding properties in endometrial epithelial cells will be required to gain a better understanding of the functional role of p6 in the endometrium.…”

Distribution of tubulointerstitial nephritis antigen-like 1 and structural matrix proteins in mouse embryos during preimplantation developmentin vivoandin vitro

“…ColIV is largely considered to be a structural component of BM, where it forms a scaffold with which other BM components, such as LN or FN, can associate (Laurie et al, 1986), and it also mediates various cell functions directly (Murray et al, 1979;Rubin et al, 1981;Aumailley & Timpl, 1986). Although some investigations into the expression of FN (Zetter & Martin, 1978;Wartiovaara et al, 1979;Yohkaichiya et al, 1988) and ColIV (Leivo et al, 1980;Sherman et al, 1980) in preimplantation embryos have been performed, detailed analyses of their expression from blastocyst activation to just prior to implantation have not been conducted. ColIV can also support the outgrowth of primary trophoblast from the mouse blastocyst (Armant et al, 1986;Sutherland et al, 1988).…”