Fibronectin levels in male ejaculate and evidence for its role in unexplained infertility

Stauffer, C; Parsons, C. Lowell

doi:10.1016/0090-4295(89)90168-4

Cited by 8 publications

(6 citation statements)

References 13 publications

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“…Wennemuth et al (2000) have reported on a significant negative correlation between FN concentration and total sperm motility. Clinical studies of Stauffer and Parsons (1989) and Wennemuth et al (2000) have indicated that determination of FN concentration in seminal plasma can be recommended as an additional diagnostic tool in andrology.The aim of this study was to identify degradation products of FN in the seminal plasma and then to determine if the presence of FN fragments recognized by a monoclonal antibody directed to the central cellular domain of FN could be associated with the results of semen evaluation. Western immunobloĴing was used to visualize the molecular masses and the range of FN fragmentation.…”

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confidence: 99%

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Fibronectin fragments in human seminal plasma.

Kątnik-Prastowska¹,

Przybysz²,

Chełmońska-Soyta³

2005

Acta Biochim Pol

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The study has revealed the presence of fibronectin (FN) fragments and a lack of intact FN in 72 seminal plasma samples. The FN fragmentation was examined by immunoblotting with a monoclonal antibody specific to the central cellular FN domain and was confirmed with a monoclonal antibody directed to the C-terminal domain of FN. Nine FN fragments between 60 and 200 kDa and five fragments of 60-150 kDa were identified in seminal plasma samples of normozoospermic and of terato-, oligoterato-, and oligoasthenoterato-spermic groups, respectively. The relative amounts of the 60, 90 and 100 kDa FN fragments were 2-3 times higher in seminal plasmas with abnormal semen characteristics than in the normozoospermic group. The results suggest that seminal plasma FN fragments may contribute to fertilization and the analysis of FN fragmentation may have a diagnostic value in andrological investigations.

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confidence: 99%

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confidence: 99%

Fibronectin fragments in human seminal plasma.

Kątnik-Prastowska¹,

Przybysz²,

Chełmońska-Soyta³

2005

Acta Biochim Pol

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“…Stauffer & Parsons (1989) provided evidences that fibronectin is present in semen bounded to spermatozoa in great concentrations in individuals having undergone surgical insult and may contribute to male infertility particularly by sperm agglutination. Pinke et al (1997) localised fibronectin on human spermatozoa correlating its distribution with their morphological and functional integrity.…”

Section: Discussionmentioning

confidence: 98%

“…This study showed significant increase of seminal fibronectin among different studied infertile groups compared with the fertile controls and a significant negative correlation with sperm concentration and total sperm motility. Stauffer & Parsons (1989) provided evidences that fibronectin is present in semen bounded to spermatozoa in great concentrations in individuals having undergone surgical insult and may contribute to male infertility particularly by sperm agglutination. Pinke et al (1997) localised fibronectin on human spermatozoa correlating its distribution with their morphological and functional integrity.…”

Section: Discussionmentioning

confidence: 99%

“…Fibronectin together with other matrix proteins is a factor mediating the sperm-egg adhesion operating as ligand between sperm surface molecules that are mainly expressed after the acrosome reaction, with special receptor proteins of zona pellucida (Calvete et al, 1990;Fusi & Bronson, 1992). Fibronectin was also demonstrated in seminal plasma where the ejaculates of a randomly selected population of men were found to display debated variation in concentration and its relationship (Stauffer & Parsons, 1989;Wennemuth et al, 1997Wennemuth et al, , 2001.…”

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confidence: 98%

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Seminal fibronectin in fertile and infertile males

Attia¹,

Hassan

Zalata

et al. 2011

Andrologia

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This study aimed to assess seminal plasma fibronectin in fertile and infertile males. Ninety infertile males were investigated; asthenozoospermia (n = 27), asthenoteratozoospermia (n = 30), oligoasthenoteratozoospermia (n = 33) compared with 20 healthy fertile controls. They were subjected to semen analysis, seminal plasma fibronectin estimation by radial immune diffusion, serum testosterone (T) and follicle stimulating hormone (FSH) estimation by ELISA. There was significant increase of seminal plasma fibronectin among different infertile groups compared with the controls. Significant negative correlation was elicited between seminal fibronectin and sperm count, sperm motility grades A, B, A + B, sperm velocity, linear velocity, linearity index, sperm normal forms and serum T. Seminal fibronectin showed significant positive correlation with grade D sperm motility and serum FSH. ROC curve analysis discriminating controls and other infertile groups demonstrated criteria value of < 674 mg l(-1) (sensitivity 100% and specificity 96.4%). It is concluded that increased seminal fibronectin is associated with decreased sperm count and sperm motility.

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