Fibronectin–hyaluronic acid composite hydrogels for three-dimensional endothelial cell culture

Seidlits, Stephanie K.; Drinnan, Charles T.; Petersen, Rebecca R.; Shear, Jason B.; Suggs, Laura J.; Schmidt, Christine E.

doi:10.1016/j.actbio.2011.03.024

Cited by 95 publications

(69 citation statements)

References 62 publications

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“…A network of interconnecting and communicating pores facilitate angiogenesis and obviates the need for matrix digestion by endothelial cells. Angiogenesis could be further promoted by enriching the hydrogel environment and introducing elements of ECM matrix to the hydrogels, such as fibronectin [134] or fibronectin-derived synthetic polypeptide motifs [135]. Incorporation of slowreleasing proangiogenic factors, such as VEGF or bFGF, is another means to potentiate formation of new vessels inside a hydrogel matrix [136].…”

Section: Angiogenesismentioning

confidence: 99%

Biomaterials Application in Stem Cell Therapies for Stroke

Moshayedi

Carmichael

2015

Cell Therapy for Brain Injury

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Section: Angiogenesismentioning

confidence: 99%

Biomaterials Application in Stem Cell Therapies for Stroke

Moshayedi

Carmichael

2015

Cell Therapy for Brain Injury

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“…If this intervention resulted in an appropriately inductive Wnt5a gradient, hESC-CM grafts isolated in scar tissue might be drawn toward viable host muscle and/or better-coupled graft implants in the border zone. Migration might be also facilitated by the delivery of hESC-CMs within FN-or Col VI-based hydrogels [52,53].…”

Section: Discussionmentioning

confidence: 99%

Human Embryonic Stem Cell-Derived Cardiomyocytes Migrate in Response to Gradients of Fibronectin and Wnt5a

Moyes

Sip

Obenza

et al. 2013

Stem Cells and Development

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An improved understanding of the factors that regulate the migration of human embryonic stem cell-derived cardiomyocytes (hESC-CMs) would provide new insights into human heart development and suggest novel strategies to improve their electromechanical integration after intracardiac transplantation. Since nothing has been reported as to the factors controlling hESC-CM migration, we hypothesized that hESC-CMs would migrate in response to the extracellular matrix and soluble signaling molecules previously implicated in heart morphogenesis. To test this, we screened candidate factors by transwell assay for effects on hESC-CM motility, followed by validation via live-cell imaging and/or gap-closure assays. Fibronectin (FN) elicited a haptotactic response from hESC-CMs, with cells seeded on a steep FN gradient showing nearly a fivefold greater migratory activity than cells on uniform FN. Studies with neutralizing antibodies indicated that adhesion and migration on FN are mediated by integrins a-5 and a-V. Next, we screened 10 soluble candidate factors by transwell assay and found that the noncanonical Wnt, Wnt5a, elicited an approximately twofold increase in migration over controls. This effect was confirmed using the gap-closure assay, in which Wnt5a-treated hESC-CMs showed approximately twofold greater closure than untreated cells. Studies with microfluidic-generated Wnt5a gradients showed that this factor was chemoattractive as well as chemokinetic, and Wnt5a-mediated responses were inhibited by the Frizzled-1/2 receptor antagonist, UM206. In summary, hESC-CMs show robust promigratory responses to FN and Wnt5a, findings that have implications on both cardiac development and cell-based therapies.

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“…18 Recent approaches have included the delivery of signaling molecules, such as simvastatin, 80 vascular endothelial growth factor, 79,84 platelet-derived growth factor, 84 transforming growth factor beta-1 (TGF-b1), 77 TGF-b3, 76 bone morphogenetic protein-2 (BMP-2), 79 phosphatidylserine, 78 and fibronectin. 85 Bae et al 80 fabricated HA hydrogels loaded with simvastatin prior to photocrosslinking to entrap the molecule within the entangled gel matrix. Most researchers utilized the ability to control molecule delivery within HA scaffolds by modulating properties, such as molecular weight, crosslinking, and scaffold formulation, accordingly.…”

Section: Hyaluronic Acidmentioning

confidence: 99%