Fibronectin during CAPD-Related Peritonitis: No Indications for Intraperitoneal Production

Zemel, Désirée; Koomen, G. C. M.; Struijk, Dirk G.; Krediet, Raymond T.

doi:10.1159/000170131

Cited by 4 publications

(1 citation statement)

References 8 publications

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“…Fibronectin can be detected in large quantities in body fluids, including CAPD effluent (20 -23). The greatest portion of fibronectin in the peritoneal cavity is probably derived from the circulation (23). However, it is also produced by both mesothe- lial cells (8,24,25) and peritoneal macrophages (22).…”

Section: Discussionmentioning

confidence: 99%

Prolonged Exposure to Glucose Degradation Products Impairs Viability and Function of Human Peritoneal Mesothelial Cells

Witowski¹,

Wiśniewska²,

Korybalska³

et al. 2001

Journal of the American Society of Nephrology

164

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Abstract. Bioincompatibility of peritoneal dialysis fluids (PDF) has been linked to the presence of glucose degradation products (GDP). Previous experiments have shown that short-term exposure to several GDP at concentrations found in commercially available PDF had no significant effect on human peritoneal mesothelial cells (HPMC). During continuous ambulatory peritoneal dialysis, however, cells are continually exposed to GDP for extended periods of time. Thus, the impact of GDP on HPMC during long-term exposure was assessed. HPMC were cultured for up to 36 d in the presence of 6 identified GDP (acetaldehyde, formaldehyde, furaldehyde, glyoxal, methylglyoxal, and 5-HMF) at doses that reflect their concentrations in conventional PDF. At regular time intervals, the ability of HPMC to secrete cytokines (interleukin-6 [IL-6]) and extracellular matrix molecules (fibronectin) was evaluated. In addition, cell viability, morphology, and proliferative potential were assessed. Exposure to GDP resulted in a significant reduction in mesothelial IL-6 and fibronectin release. Approximately 80% of this decrease occurred during the first 12 d of the exposure and was paralleled by a gradual loss of cell viability and development of morphologic alterations. After 36 d of exposure, the number of cells in GDP-treated cultures was reduced by nearly 60%. However, GDP-treated cells were able to resume normal proliferation when transferred to a normal GDP-free medium. HPMC viability and function may be impaired during long-term exposure to clinically relevant concentrations of GDP, which suggests a potential role of GDP in the pathogenesis of peritoneal membrane dysfunction during chronic peritoneal dialysis.

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Section: Discussionmentioning

confidence: 99%

Prolonged Exposure to Glucose Degradation Products Impairs Viability and Function of Human Peritoneal Mesothelial Cells

Witowski¹,

Wiśniewska²,

Korybalska³

et al. 2001

Journal of the American Society of Nephrology

164

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Host defences in continuous ambulatory peritoneal dialysis and the genesis of peritonitis

Cameron¹

1995

Pediatr Nephrol

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Continuous ambulatory peritoneal dialysis (CAPD) has come to be extensively used for the treatment of end-stage renal failure in children, and especially infants, such that now more than half of children on dialysis worldwide receive treatment by this means. Peritonitis, however, is commoner in children than in adults receiving treatment, and is a major source of morbidity and treatment failure in children started on CAPD. Only recently has the immunology of the normal peritoneum been studied extensively, with the need to assess the impact of the installation of large volumes of fluid into the peritoneal sac during dialysis. The main phagocytic defences of the peritoneum depend upon a unique set of macrophages which are present free in the peritoneal fluid but also in the submesothelium and in perivascular collections together with B lymphocytes in the submesothelial area. Both the number of macrophages per unit volume and the concentration of opsonic proteins, such as IgG, complement and fibronectin, are reduced to between only 1% and 5% when dialysis fluid is continuously present in the peritoneal sac. In addition, the fluids used for CAPD are toxic to both macrophages and to mesothelial cells. Thus minor degrees of contamination frequently lead to peritonitis and in addition the majority of patients have catheters inserted in their peritoneum which become colonised with organisms capable of producing exopolysaccharide (slime), which promotes adhesion of the organism to the plastic and protects them against phagocytic attack and the penetration of antibiotics. Thus the peritoneum is in a state of continual inflammation, as well as being a markedly more vulnerable site than the normal peritoneum to the entry of organisms. Whether clinical peritonitis appears in this state of chronic contamination probably depends on perturbation in the balance between host defences and the organism. Whilst Staphylococcus epidermidis is the commonest cause of peritonitis, Staphylococcus aureus and Gram-negative organisms are much more serious and more frequently lead either to temporary catheter removal or discontinuation of dialysis altogether. This review describes the peritoneal defences in relation to the genesis of peritonitis.

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Expression of adhesion molecules and fibronectin of activated peritoneal surface with lipopolysaccharide (LPS) analyzed with immuno SEM

Liang

Jyoukura

Ogiwara

et al. 2001

Annals of Anatomy - Anatomischer Anzeiger

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Fibronectin during CAPD-Related Peritonitis: No Indications for Intraperitoneal Production

Cited by 4 publications

References 8 publications

Prolonged Exposure to Glucose Degradation Products Impairs Viability and Function of Human Peritoneal Mesothelial Cells

Prolonged Exposure to Glucose Degradation Products Impairs Viability and Function of Human Peritoneal Mesothelial Cells

Host defences in continuous ambulatory peritoneal dialysis and the genesis of peritonitis

Expression of adhesion molecules and fibronectin of activated peritoneal surface with lipopolysaccharide (LPS) analyzed with immuno SEM

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