Fibronectin distribution in the chick embryo during formation of the blastula

Raddatz, Eric; Monnet‐Tschudi, Florianne; Verdan, C; Kučera, Pavel

doi:10.1007/bf00185835

Cited by 8 publications

(6 citation statements)

References 40 publications

(27 reference statements)

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“…Expression at the anterior (rostral) end of the AO/AP border in the germinal crescent where PGCs congregate contained a dense meshwork of FN fibrils (Figures 4A, 5A). In agreement with earlier studies, the expression pattern of FN in the embryonic tissue layers was less dense than at the AO/AP border, consisting of short, scattered fibrils and puncta (Duband and Thiery, 1982; Raddatz et al, 1991).…”

Section: Resultssupporting

confidence: 92%

Avian Primordial Germ Cells Contribute to and Interact With the Extracellular Matrix During Early Migration

Huss

Saias

Hamamah

et al. 2019

Front. Cell Dev. Biol.

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During early avian development, primordial germ cells (PGC) are highly migratory, moving from the central area pellucida of the blastoderm to the anterior extra-embryonic germinal crescent. The PGCs soon move into the forming blood vessels by intravasation and travel in the circulatory system to the genital ridges where they participate in the organogenesis of the gonads. This complex cellular migration takes place in close association with a nascent extracellular matrix that matures in a precise spatio-temporal pattern. We first compiled a list of quail matrisome genes by bioinformatic screening of human matrisome orthologs. Next, we used single cell RNA-seq analysis (scRNAseq) to determine that PGCs express numerous ECM and ECM-associated genes in early embryos. The expression of select ECM transcripts and proteins in PGCs were verified by fluorescent in situ hybridization (FISH) and immunofluorescence (IF). Live imaging of transgenic quail embryos injected with fluorescent antibodies against fibronectin and laminin, showed that germinal crescent PGCs display rapid shape changes and morphological properties such as blebbing and filopodia while surrounded by, or in close contact with, an ECM fibril meshwork that is itself in constant motion. Injection of anti-β1 integrin CSAT antibodies resulted in a reduction of mature fibronectin and laminin fibril meshwork in the germinal crescent at HH4-5 but did not alter the active motility of the PGCs or their ability to populate the germinal crescent. These results suggest that integrin β1 receptors are important, but not required, for PGCs to successfully migrate during embryonic development, but instead play a vital role in ECM fibrillogenesis and assembly.

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Section: Resultssupporting

confidence: 92%

Avian Primordial Germ Cells Contribute to and Interact With the Extracellular Matrix During Early Migration

Huss

Saias

Hamamah

et al. 2019

Front. Cell Dev. Biol.

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“…Thus, full protection may require a full-length or larger recombinant Embp that can both bind viruses and reduce infectivity. Fibronectin is known to be present in the chick embryo 37 . However, chicken and mammal fibronectins are differentially glycosylated 38 .…”

Section: Discussionmentioning

confidence: 99%

Nasal commensal Staphylococcus epidermidis counteracts influenza virus

Chen

Liu

et al. 2016

Sci Rep

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Several microbes, including Staphylococcus epidermidis (S. epidermidis), a Gram-positive bacterium, live inside the human nasal cavity as commensals. The role of these nasal commensals in host innate immunity is largely unknown, although bacterial interference in the nasal microbiome may promote ecological competition between commensal bacteria and pathogenic species. We demonstrate here that S. epidermidis culture supernatants significantly suppressed the infectivity of various influenza viruses. Using high-performance liquid chromatography together with mass spectrometry, we identified a giant extracellular matrix-binding protein (Embp) as the major component involved in the anti-influenza effect of S. epidermidis. This anti-influenza activity was abrogated when Embp was mutated, confirming that Embp is essential for S. epidermidis activity against viral infection. We also showed that both S. epidermidis bacterial particles and Embp can directly bind to influenza virus. Furthermore, the injection of a recombinant Embp fragment containing a fibronectin-binding domain into embryonated eggs increased the survival rate of virus-infected chicken embryos. For an in vivo challenge study, prior Embp intranasal inoculation in chickens suppressed the viral titres and induced the expression of antiviral cytokines in the nasal tissues. These results suggest that S. epidermidis in the nasal cavity may serve as a defence mechanism against influenza virus infection.

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“…However, the level of expression may be very low and we could not detect the expression of laminin alpha1 in our transcriptome screen or by RNA in situ analysis. Although subtype specificity is often difficult to discern from immunostaining, the presence of laminin and several other BM components at protein group levels has been reported for gastrulation stage chick embryos [Sanders, 1979[Sanders, , 1984Raddatz et al, 1991;Soulintzi and Zagris, 2007;Nakaya and Sheng, 2009].…”

Section: Resultsmentioning

confidence: 99%

Involvement of Dystroglycan in Epithelial-Mesenchymal Transition during Chick Gastrulation

Nakaya¹,

Sukowati²,

Alev³

et al. 2010

Cells Tissues Organs

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Regulated disruption of the basement membrane (BM) is a critical step in many epithelial-mesenchymal transition (EMT) processes. Molecular mechanisms controlling the interaction between the BM and the basal membrane of epithelial cells and its subsequent disruption during EMT are poorly understood. Using chick embryos as a model, we analyzed the molecular complexity of this interaction during gastrulation EMT. Transcriptome data indicated that the BM of the gastrulation stage chick epiblast contains a full range of BM component proteins with unique subtype combinations. Integrins and dystroglycan are 2 major groups of basal membrane proteins involved in BM interaction. We provide evidence that dystroglycan gene expression is restricted to the epiblast during early development and its expression is downregulated in cells undergoing gastrulation EMT. The β-dystroglycan protein is localized to the basolateral membrane in epiblast cells and the basal localization is lost in cells undergoing EMT. Disruption of actin filaments leads to a decrease in the lateral membrane localization of β-dystroglycan and a relative increase in basal membrane localization, whereas disruption of microtubules leads to the loss of BM/basal membrane interaction and basal membrane β-dystroglycan localization. Overall, these data suggest an involvement of dystroglycan, especially the regulation of its expression and localization, in gastrulation EMT.

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Fibronectin distribution in the chick embryo during formation of the blastula

Cited by 8 publications

References 40 publications

Avian Primordial Germ Cells Contribute to and Interact With the Extracellular Matrix During Early Migration

Avian Primordial Germ Cells Contribute to and Interact With the Extracellular Matrix During Early Migration

Nasal commensal Staphylococcus epidermidis counteracts influenza virus

Involvement of Dystroglycan in Epithelial-Mesenchymal Transition during Chick Gastrulation

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