“…Epidermal specimens were fixed in 4% paraformaldehyde and embedded in paraffin . After blocking with 3% bovine serum albumin, sections were incubated with primary antibodies to metallothionein (MT) (1 : 100; Abcam), proliferating cell nuclear antigen (1 : 100; Dako, Glostrup, Denmark), E‐cadherin (1 : 100; Santa Cruz Biotechnology, Santa Cruz, CA, U.S.A.), zona occludens (ZO)‐1 (1 : 100; Invitrogen, Carlsbad, CA, U.S.A.), keratin 1 (1 : 100; Abcam), keratin 14 (1 : 100; Abcam) and involucrin (1 : 100; Neomarkers, Fremont, CA, U.S.A.).…”