Fibronectin Binding to the
            <i>Salmonella enterica</i>
            Serotype Typhimurium ShdA Autotransporter Protein Is Inhibited by a Monoclonal Antibody Recognizing the A3 Repeat

Kingsley, Robert A.; Ghanem, Daad Abi; Puebla-Osorio, Nahum; Keestra, A. Marijke; Berghman, Luc; Bäumler, Andreas J.

doi:10.1128/jb.186.15.4931-4939.2004

Cited by 41 publications

(40 citation statements)

References 37 publications

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“…2A). Such a disparity in mass has been observed previously for proteins containing repeated acidic sequences, including the AT protein ShdA from Salmonella enterica (29). UpaH is also highly acidic, containing 376/2,845 (13.2%) acidic amino acids (glutamate and aspartate).…”

Section: Resultsmentioning

confidence: 78%

UpaH Is a Newly Identified Autotransporter Protein That Contributes to Biofilm Formation and Bladder Colonization by Uropathogenic Escherichia coli CFT073

et al. 2010

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Escherichia coli is the primary cause of urinary tract infection (UTI) in the developed world. The major factors associated with virulence of uropathogenic E. coli (UPEC) are fimbrial adhesins, which mediate specific attachment to host receptors and trigger innate host responses. Another group of adhesins is represented by the autotransporter (AT) subgroup of proteins. In this study, we identified a new AT-encoding gene, termed upaH, present in a 6.5-kb unannotated intergenic region in the genome of the prototypic UPEC strain CFT073. Cloning and sequencing of the upaH gene from CFT073 revealed an intact 8.535-kb coding region, contrary to the published genome sequence. The upaH gene was widely distributed among a large collection of UPEC isolates as well as the E. coli Reference (ECOR) strain collection. Bioinformatic analyses suggest ␤-helix as the predominant structure in the large N-terminal passenger (␣) domain and a 12-strand ␤-barrel for the C-terminal ␤-domain of UpaH. We demonstrated that UpaH is expressed at the cell surface of CFT073 and promotes biofilm formation. In the mouse UTI model, deletion of the upaH gene in CFT073 and in two other UPEC strains did not significantly affect colonization of the bladder in single-challenge experiments. However, in competitive colonization experiments, CFT073 significantly outcompeted its upaH isogenic mutant strain in urine and the bladder.

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Section: Resultsmentioning

confidence: 78%

UpaH Is a Newly Identified Autotransporter Protein That Contributes to Biofilm Formation and Bladder Colonization by Uropathogenic Escherichia coli CFT073

et al. 2010

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“…(230) and motility in Myxococcus xanthus (231). ShdA is a protein that is involved in the colonization of Peyer's patches by S. Typhimurium and the shedding of the bacteria following infection (232,233). The presence of a larger number of functional genes in broader-host-range serovars such as S. Enteritidis likely contributed to the ability to colonize and infect a greater number of hosts (234).…”

Section: Examples Of Salmonella Host Range In Chicken-related Serovarsmentioning

confidence: 99%

Salmonella Pathogenicity and Host Adaptation in Chicken-Associated Serovars

Foley

Johnson

Ricke

et al. 2013

Microbiol Mol Biol Rev

262

218

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SUMMARY Enteric pathogens such as Salmonella enterica cause significant morbidity and mortality. S. enterica serovars are a diverse group of pathogens that have evolved to survive in a wide range of environments and across multiple hosts. S. enterica serovars such as S . Typhi, S . Dublin, and S . Gallinarum have a restricted host range, in which they are typically associated with one or a few host species, while S . Enteritidis and S . Typhimurium have broad host ranges. This review examines how S. enterica has evolved through adaptation to different host environments, especially as related to the chicken host, and continues to be an important human pathogen. Several factors impact host range, and these include the acquisition of genes via horizontal gene transfer with plasmids, transposons, and phages, which can potentially expand host range, and the loss of genes or their function, which would reduce the range of hosts that the organism can infect. S . Gallinarum, with a limited host range, has a large number of pseudogenes in its genome compared to broader-host-range serovars. S. enterica serovars such as S . Kentucky and S . Heidelberg also often have plasmids that may help them colonize poultry more efficiently. The ability to colonize different hosts also involves interactions with the host's immune system and commensal organisms that are present. Thus, the factors that impact the ability of Salmonella to colonize a particular host species, such as chickens, are complex and multifactorial, involving the host, the pathogen, and extrinsic pressures. It is the interplay of these factors which leads to the differences in host ranges that we observe today.

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“…In contrast to other adhesins, which exclusively bind fibronectin and have evolved to bind this ligand with exceptionally high affinity, [20][21][22] MAM7 seems to bind to fibronectin relatively weakly (K D of 15 mM). This low affinity interaction is complemented by a second receptor, phosphatidic acid, resulting in an overall affinity of MAM7 for host cells that is very high (approx.…”

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confidence: 99%