Fibronectin-Binding Proteins of
            <i>Staphylococcus aureus</i>
            Are Involved in Adherence to Human Airway Epithelium

Mongodin, Emmanuel F.; Bajolet, Odile; Cutrona, Jérôme; Bonnet, N.; Dupuit, Florence; Puchelle, Édith; Bentzmann, Sophie de

doi:10.1128/iai.70.2.620-630.2002

Cited by 99 publications

(57 citation statements)

References 43 publications

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“…Other commensals, such as H. influenzae, can colonize airway epithelia at the ALI for days in vitro with no evidence of detrimental effects (Starner et al, 2006). Most reports of S. aureus in vitro interactions with epithelia cell lines use brief incubation times (less than 4 h) to examine adherence (Mongodin et al, 2000;Mongodin et al, 2002;Escotte et al, 2006), although interactions at time points of up to 20 h have been reported using low inocula with no damage to the airway cells (Quinn and Cole, 2007). The reason for these short-term experiments could be due to the in vitro toxicity of S. aureus that results from alphatoxin.…”

Section: Discussionmentioning

confidence: 99%

“…In human studies, ClfB was again found to be necessary for colonization (Wertheim et al, 2008), supporting in vitro studies and prompting development of ClfB as a vaccine candidate (Schaffer et al, 2006). One drawback of most studies utilizing human cell lines is that S. aureus exposure to airway epithelial cells in vitro is brief, addressing initial attachment rather than long-term colonization (Mongodin et al, 2002;O'Brien et al, 2002;Roche et al, 2003;Corrigan et al, 2009). Aside from the role of specific adhesins or binding proteins, these studies provide little information on the physiological state of the bacterial cells in this host niche.…”

Section: Introductionmentioning

confidence: 91%

“…Clumping factor B (ClfB) is required to bind type I cytokeratin 10 on desquamated nasal epithelia (O'Brien et al, 2002;Walsh et al, 2004). The surface adhesins SasG (Roche et al, 2003), IsdA (Corrigan et al, 2009), SdrC and SdrD (Corrigan et al, 2009), as well as the fibronectin binding proteins (Mongodin et al, 2002), have also been demonstrated to have roles in adherence to host cells. ClfB and IsdA are essential in rodent models of nasal colonization (Clarke et al, 2006;Schaffer et al, 2006), and not surprisingly, the extracellular enzyme that localizes all of these surface adhesins to the cell wall, sortase (SrtA), is also required for colonization (Schaffer et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Development of an in vitro colonization model to investigateStaphylococcus aureusinteractions with airway epithelia

Kiedrowski

Paharik

Ackermann

et al. 2016

Cellular Microbiology

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SUMMARY Staphylococcus aureus is a bacterial pathogen responsible for a wide range of diseases and is also a human commensal colonizing the upper respiratory tract. Strains belonging to the clonal complex group CC30 are associated with colonization, although the colonization state itself is not clearly defined. In this work, we developed a co-culture model with S. aureus colonizing the apical surface of polarized human airway epithelial cells. The S. aureus are grown at the air-liquid interface to allow an in-depth evaluation of a simulated colonization state. Exposure to wild-type S. aureus bacteria or conditioned media killed airway epithelial cells within one day, while mutant S. aureus strains lacking alpha-toxin (hla) persisted on viable cells for at least two days. Recent S. aureus CC30 isolates are natural hla mutants, and we observed that these strains displayed reduced toxicity toward airway epithelial cells. Quantitative real-time PCR of known virulence factors showed the expression profile of S. aureus grown in co-culture correlates with results from previous human colonization studies. Microarray analysis indicated significant shifts in S. aureus physiology in the co-culture model toward lipid and amino acid metabolism. The development of the in vitro colonization model will enable further study of specific S. aureus interactions with the host epithelia.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Development of an in vitro colonization model to investigateStaphylococcus aureusinteractions with airway epithelia

Kiedrowski

Paharik

Ackermann

et al. 2016

Cellular Microbiology

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show abstract

“…Variations in binding between strains of S. aureus have been shown to, for example, collagen, 28 and fibronectin. [29][30][31] Further extensive studies are required to investigate and elucidate possible differences in adherence between strains of S. intermedius.…”

Section: Discussionmentioning

confidence: 99%

Species specificity in the adherence of staphylococci to canine and human corneocytes: a preliminary study

Simou

Hill

Forsythe³

et al. 2005

Veterinary Dermatology

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Staphylococcal pyoderma is rarely contagious between dogs and humans, or humans and dogs. This study investigated the hypothesis that there are species differences in adherence of Staphylococcus intermedius (the most common isolate from dogs) and Staphylococcus aureus (the most common isolate from humans) to canine and human corneocytes. Sheets of corneocytes were collected from the ventral abdomen of 10 dogs and the medial forearm of 10 humans (all healthy and without any history or physical signs of skin disease) using double-sided tape. Staphylococcus intermedius from a case of canine bacterial pyoderma and a human strain of S. aureus were prepared in phosphate buffered saline (PBS) and applied in duplicate, respectively, to the canine and human corneocyte-covered tapes using PBS as negative control. After incubation, rinsing, and staining with crystal violet, quantification of the adherent bacteria was carried out blindly by computerized image analysis. Staphylococcus intermedius was found to adhere significantly more to canine corneocytes than S. aureus (P = 0.0006), whereas S. aureus showed greater adherence to human corneocytes than S. intermedius (P < 0.0001). In addition, the pattern of adherence differed between the two organisms, with S. intermedius adhering to the entire surface and S. aureus adhering mainly to the periphery of both canine and human corneocytes. Preference for adherence to these two hosts may explain, in part, why S. intermedius and S. aureus are uncommonly isolated from human and canine skin infections, respectively.

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“…Mongodin et al [5] demonstrated the major role of FnBPs in S. aureus adherence to human airway epithelium, whereas staphylococcal protein A and clumping factor did not play any role in this process. These authors also reported that 97% of 32 clinical strains, isolated from the airway secretions of patients with cystic fibrosis and nosocomial pneumonia, possessed the two fnb genes.…”

Section: Discussionmentioning

confidence: 99%

Distribution of virulence genes ofStaphylococcus aureusisolated from stable nasal carriers

Nashev

Toshkova

Salasia

et al. 2004

FEMS Microbiology Letters

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In the present study, we report data on virulence determinants of Staphylococcus aureus from stable nasal carriers, emphasizing on the genes encoding fibronectin (fnbA, fnbB) and collagen (cna) adhesive molecules. Of the 44 S. aureus isolates included, 32 isolates (16 pairs) were cultured from the anterior nares of 16 healthy carriers, eight isolates (four pairs) were collected from the nose of four patients with recurrent skin infections and four isolates were obtained from the infection site of these patients. The period between the two nasal swabs taken was 3-5 days. The persistency of carriage could be demonstrated by the indistinguishable genotypic characteristics of the S. aureus isolates in each pair. This could be shown by determination of gene polymorphisms of coa gene and the X-region and IgG-binding region encoding segments of spa gene. In addition, the isolates within the pairs showed identical toxin patterns. This was determined by PCR amplification of the genes encoding staphylococcal enterotoxins (SEA to SEJ) and TSST-1. The genotypic properties also yielded an identity between persistent nasal carriage isolates and the corresponding skin infection isolates of the four patients. In addition, all S. aureus nasal and skin infection isolates were positive for gene fnbA, fnbB and cna could be found with a high frequency. Among the 44 isolates investigated, 16 isolates (36.7%) harbored gene fnbB and 21 isolates (47.7%) gene cna. The data in the present study showed a relatively wide distribution of the genes fnb and cna among the investigated isolates, indicating that the persistent carriage of strains harboring these virulence determinants may increase the risk for subsequent invasive infections in carriers.

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Fibronectin-Binding Proteins of Staphylococcus aureus Are Involved in Adherence to Human Airway Epithelium

Cited by 99 publications

References 43 publications

Development of an in vitro colonization model to investigateStaphylococcus aureusinteractions with airway epithelia

Development of an in vitro colonization model to investigateStaphylococcus aureusinteractions with airway epithelia

Species specificity in the adherence of staphylococci to canine and human corneocytes: a preliminary study

Distribution of virulence genes ofStaphylococcus aureusisolated from stable nasal carriers

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