Fibronectin assembly during early embryo development: A versatile communication system between cells and tissues

Almeida, Patrícia Gomes de; Pinheiro, Gonçalo G.; Nunes, Andreia; Gonçalves, André; Þorsteinsdóttir, Sólveig

doi:10.1002/dvdy.24391

Cited by 29 publications

(21 citation statements)

References 77 publications

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“…As an EMT marker, fibronectin is involved in cell adhesion and migration, including embryogenesis,17 wound healing,18 blood coagulation,19 osteogenesis,20 and metastasis 21,22. EMT is a process involved in embryonic development, during which cells lose their epithelial features and adopt characteristics of mesenchymal cells.…”

Section: Discussionmentioning

confidence: 99%

Fibronectin 1 promotes migration and invasion of papillary thyroid cancer and predicts papillary thyroid cancer lymph node metastasis

Xia¹,

Postma²,

Yang³

et al. 2017

OTT

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Lymph node metastasis (LNM) is common in papillary thyroid cancer (PTC), and is an indicator of recurrence. The detailed molecular mechanism of LNM in PTC has not been well described. This study aimed to investigate the role of fibronectin 1 in PTC LNM and its clinical relevance. The expression of fibronectin 1 was confirmed in PTC tissues and cell lines. A correlation analysis was conducted and a receiver-operating characteristic curve obtained. The effect of fibronectin 1 on the proliferation of PTC cell lines was performed using a colony-formation assay and Cell Counting Kit 8. Cell-cycle analysis was performed with a flow-cytometry assay. Migration and invasion ability were evaluated by transwell and wound-healing assays. Fibronectin 1 was overexpressed in metastasized PTC. Overexpressed fibronectin 1 was positively correlated with PTC LNM. Receiver-operating characteristic analysis showed that the diagnostic accuracy of fibronectin 1 was 81.1%, with sensitivity of 80% and specificity of 82%. Overexpression of fibronectin 1 promoted proliferation, migration, and invasion in PTC. Fibronectin 1 plays a critical role in PTC metastasis by modulating the proliferation, migration, and invasion ability of PTC cells, and it is a valuable diagnostic biomarker for predicting PTC LNM.

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Section: Discussionmentioning

confidence: 99%

Fibronectin 1 promotes migration and invasion of papillary thyroid cancer and predicts papillary thyroid cancer lymph node metastasis

Xia¹,

Postma²,

Yang³

et al. 2017

OTT

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“…Fibronectin has been demonstrated to stimulate human sperm capacitation by activating the protein kinase A pathway [ 110 ]. Organization of the extracellular matrix and paracrine communication by fibronectin has been identified to be important for early embryogenesis [ 111 , 112 ]. Zinc-α-2-glycoprotein has been demonstrated to induce cAMP signaling and modulate motility in human sperm [ 113 ].…”

Section: Resultsmentioning

confidence: 99%

Profiling of proteins secreted in the bovine oviduct reveals diverse functions of this luminal microenvironment

et al. 2017

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The oviductal microenvironment is a site for key events that involve gamete maturation, fertilization and early embryo development. Secretions into the oviductal lumen by either the lining epithelium or by transudation of plasma constituents are known to contain elements conducive for reproductive success. Although previous studies have identified some of these factors involved in reproduction, knowledge of secreted proteins in the oviductal fluid remains rudimentary with limited definition of function even in extensively studied species like cattle. In this study, we used a shotgun proteomics approach followed by bioinformatics sequence prediction to identify secreted proteins present in the bovine oviductal fluid (ex vivo) and secretions from the bovine oviductal epithelial cells (in vitro). From a total of 2087 proteins identified, 266 proteins could be classified as secreted, 109 (41%) of which were common for both in vivo and in vitro conditions. Pathway analysis indicated different classes of proteins that included growth factors, metabolic regulators, immune modulators, enzymes, and extracellular matrix components. Functional analysis revealed mechanisms in the oviductal lumen linked to immune homeostasis, gamete maturation, fertilization and early embryo development. These results point to several novel components that work together with known elements mediating functional homeostasis, and highlight the diversity of machinery associated with oviductal physiology and early events in cattle fertility.

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“…The development and regeneration of muscle tissue is intimately linked to changes in the expression of the integrin family of receptors and their association with different ECM structures. During development, myoblasts of somitic origin (Chevallier, 1979) invade the limb mesenchyme along migration pathways that provide an ectoderm-or fibroblast-secreted FN-containing matrix (Chiquet et al, 1981;de Almeida et al, 2016). While individual myoblasts can also secrete and organize their own FN network, the FN network is lost from the surfaces of fused myotubes (Chen, 1977;Chiquet et al, 1981).…”

Section: Discussionmentioning

confidence: 99%

β1D integrin splice variant stabilizes integrin dynamics and reduces integrin signaling by limiting paxillin recruitment

Soto-Ribeiro

Kastberger

Bachmann

et al. 2019

Journal of Cell Science

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Heterodimeric integrin receptors control cell adhesion, migration and extracellular matrix assembly. While the α integrin subunit determines extracellular ligand specificity, the β integrin chain binds to an acidic residue of the ligand, and cytoplasmic adapter protein families such as talins, kindlins and paxillin, to form mechanosensing cell matrix adhesions. Alternative splicing of the β1 integrin cytoplasmic tail creates ubiquitously expressed β1A, and the heart and skeletal musclespecific β1D form. To study the physiological difference between these forms, we developed fluorescent β1 integrins and analyzed their dynamics, localization, and cytoplasmic adapter recruitment and effects on cell proliferation. On fibronectin, GFP-tagged β1A integrin showed dynamic exchange in peripheral focal adhesions, and long, central fibrillar adhesions. In contrast, GFP-β1D integrins exchanged slowly, forming immobile and short central adhesions. While adhesion recruitment of GFP-β1A integrin was sensitive to C-terminal tail mutagenesis, GFP-β1D integrin was recruited independently of the distal NPXY motif. In addition, a P786A mutation in the proximal, talin-binding NPXY 783 motif switched β1D to a highly dynamic integrin. In contrast, the inverse A786P mutation in β1A integrin interfered with paxillin recruitment and proliferation. Thus, differential β1 integrin splicing controls integrin-dependent adhesion signaling, to adapt to the specific physiological needs of differentiated muscle cells.

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Fibronectin assembly during early embryo development: A versatile communication system between cells and tissues

Cited by 29 publications

References 77 publications

Fibronectin 1 promotes migration and invasion of papillary thyroid cancer and predicts papillary thyroid cancer lymph node metastasis

Fibronectin 1 promotes migration and invasion of papillary thyroid cancer and predicts papillary thyroid cancer lymph node metastasis

Profiling of proteins secreted in the bovine oviduct reveals diverse functions of this luminal microenvironment

β1D integrin splice variant stabilizes integrin dynamics and reduces integrin signaling by limiting paxillin recruitment

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