Fibronectin and laminin enhance engraftibility of cultured hematopoietic stem cells

Two-Dimensional Polymer-Based Cultures Expand Cord Blood-Derived Hematopoietic Stem Cells and Support Engraftment of NSG Mice

“…Microscopic images revealed that HSC superiorly interacted with fibrin fibers, by developing cell filopodia and adhesive characteristics. Previous studies have shown that HSC proliferation and differentiation often co-occurs with morphological changes such as substrate adherence and filopodia production, 18,27,[34][35][36] and this is in line with our findings. In addition, fibrin typically consisted of a 3D-like matrix of fibers, very likely resembling the ECM responsible for HSC maintenance in vivo.…”

Characterization of the growth modulatory activities of osteoblast conditioned media on cord blood progenitor cells

“…Osteoblast and M-OSTs secrete a wide array of matrix materials and ECM proteins such as collagen I, fibronectin, osteocalcin and osteopontin (Kulterer et al 2007;Neve et al 2011). ECM proteins have been implicated in the regulation of homing, survival, differentiation and proliferation of hematopoietic cells (Yokota et al 1998;Voermans et al 1999;Sagar et al 2006). Furthermore, growth factors and cytokines have a natural affinity for ECM proteins (Taipale and Keski-Oja 1997) and such interaction has been reported to potentiate the mitogenic and functional properties of several growth factors (Clark 2008).…”

Biophysical regulation of hematopoietic stem cells

“…They also influence HSC †, ‡ proliferation, differentiation, and the cell's ability to engraft. [18][19][20] Osteopontin -another member of the structural proteins of the bone marrow ECM -was shown to negatively regulate both the HSC pool size in vivo in mice as well as the in vitro proliferation of human HSCs. † 21,22 Proteoglycans consist of a protein core with covalently bound glycosaminoglycans.…”