Fibronectin and laminin binding of urogenital and oral Prevotella species

Eiring, Peter; Waller, Karsten; Widmann, Alexander; Werner, H.

doi:10.1016/s0934-8840(98)80009-1

Cited by 9 publications

(7 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although few other species have been shown to be able to form biofilm-like aggregates in SF ( Gilbertie et al, 2019 ), the herein encountered P. bivia neither displayed tightly adhering cells in the clinical sample nor was it able to form a sessile or planktonic biofilm in the presence of both SF and culture medium. While in the past P. bivia was shown to bind connective tissue matrix proteins such as fibronectin and laminin ( Eiring et al, 1998 ), other studies demonstrated a weak or almost absent biofilm-forming ability ( Guaglianone et al, 2010 ; Patterson et al, 2010 ). The consequences are reflected in the lack of increase of recalcitrance to antimicrobial treatment in the presence of SF, contrarily to those species that are able to aggregate.…”

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of Planktonic Biofilm-Like Aggregates in Infected Synovial Fluids From Joint Infections

et al. 2020

View full text Add to dashboard Cite

Recent in vitro studies reported the exceptional ability of some bacterial species to form biofilm-like aggregates in human and animal synovial fluids (SF), but evidences from infected clinical samples are still lacking. In this study, we investigated whether this bacterial phenotype was present in infected SFs collected from joint infections and if it was maintained in in vitro settings. SFs sent for culture to the Laboratory of Microbiology of our institute were directly analyzed by means of confocal laser scanning microscopy (CLSM), and the infective agents were isolated for further in vitro tests. Moreover, sterile SF was collected from patients who did not receive previous antibiotic therapy to investigate the formation of bacterial aggregates, together with biofilm and matrix production on a titanium surface. Finally, antibiotic susceptibility studies were performed by using bovine SF. Four Staphylococcus aureus, one Staphylococcus lugdunensis, and one Prevotella bivia strain were identified in the infected SFs. The CLSM analysis showed that all staphylococci were present as a mixture of single cells and bacterial clumps surrounded by an exopolymeric substance, which comprised SF-derived fibrin, while all P. bivia cells appeared separated. Despite that, differences in the ability to aggregate between S. aureus and S. lugdunensis were observed in clinical SFs. These different phenotypes were further confirmed by in vitro growth, even though the application of such ex vivo approach lead all staphylococci to form exceptionally large microbial aggregates, which are several folds bigger than those observed in clinical samples. Planktonic aggregates challenged for antibiotic susceptibility revealed a sharp increase of recalcitrance to the treatments. Although this is still at a preliminary stage, the present work confirmed the ability of staphylococci to form free-floating biofilm-like aggregates in infected SF from patients with joint infections. Furthermore, the obtained results pointed out that future in vitro research on joint infections will benefit from the use of human-or animal-derived SF. Even though this approach should be carefully validated in further studies comprising a larger microbial population, these findings pose new challenges in the treatment of infected native and prosthetic joints and for the approach to new investigations.

show abstract

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of Planktonic Biofilm-Like Aggregates in Infected Synovial Fluids From Joint Infections

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Prevotella intermedia has an aetiologic role in gingivitis, destructive periodontitis and root canal infections. P. intermedia binds to many ECM proteins (Kalfas et al ., ; Alugupalli & Kalfas, ; Eiring et al ., ), and recently, the surface‐exposed AdpB (29 kDa) was characterized as a high‐affinity Fn‐ and Ln‐binding protein. P. intermedia has also the ability to bind to collagen I found in teeth, and to collagen IV located in the BM, but although it was suggested that porins are involved in these interactions, no specific collagen‐binding adhesins have been unraveled so far (Alugupalli et al ., ; Grenier, ).…”

Section: Attachment Of Oral Pathogens To Ln and Collagenmentioning

confidence: 99%

Human pathogens utilize host extracellular matrix proteins laminin and collagen for adhesion and invasion of the host

et al. 2012

View full text Add to dashboard Cite

Laminin (Ln) and collagen are multifunctional glycoproteins that play an important role in cellular morphogenesis, cell signalling, tissue repair and cell migration. These proteins are ubiquitously present in tissues as a part of the basement membrane (BM), constitute a protective layer around blood capillaries and are included in the extracellular matrix (ECM). As a component of BMs, both Lns and collagen(s), thus function as major mechanical containment molecules that protect tissues from pathogens. Invasive pathogens breach the basal lamina and degrade ECM proteins of interstitial spaces and connective tissues using various ECM-degrading proteases or surface-bound plasminogen and matrix metalloproteinases recruited from the host. Most pathogens associated with the respiratory, gastrointestinal, or urogenital tracts, as well as with the central nervous system or the skin, have the capacity to bind and degrade Lns and collagen(s) in order to adhere to and invade host tissues. In this review, we focus on the adaptability of various pathogens to utilize these ECM proteins as enhancers for adhesion to host tissues or as a targets for degradation in order to breach the cellular barriers. The major pathogens discussed are Streptococcus, Staphylococcus, Pseudomonas, Salmonella, Yersinia, Treponema, Mycobacterium, Clostridium, Listeria, Porphyromonas and Haemophilus; Candida, Aspergillus, Pneumocystis, Cryptococcus and Coccidioides; Acanthamoeba, Trypanosoma and Trichomonas; retrovirus and papilloma virus.

show abstract

“…Fibronectin‐ and laminin‐binding properties are other virulence factors which have been demonstrated for Prevotella spp., particularly P. bivia (32, 33). Elastase is produced also by Fusobacterium nucleatum , a significant and potent pathogen which occurs in many types of infection.…”

Section: Virulence Factors Of Bv Pathogenesismentioning

confidence: 99%

Bacterial vaginosis – a microbiological and immunological enigma

Forsum

Holst

Larsson

et al. 2005

APMIS

View full text Add to dashboard Cite

The development of bacterial vaginosis (BV) among women of childbearing age and the resulting quantitative and qualitative shift from normally occurring lactobacilli in the vagina to a mixture of mainly anaerobic bacteria is a microbiological and immunological enigma that so far has precluded the formulation of a unifying generally accepted theory on the aetiology and clinical course of BV. This critical review highlights some of the more important aspects of BV research that could help in formulating new basic ideas respecting the biology of BV, not least the importance of the interleukin mediators of local inflammatory responses and the bacterial shift from the normally occurring lactobacilli species: L. crispatus, L. gasseri, L. jensenii, and L. iners to a mixed flora dominated by anaerobic bacteria.

show abstract

Fibronectin and laminin binding of urogenital and oral Prevotella species

Cited by 9 publications

References 30 publications

Identification and Characterization of Planktonic Biofilm-Like Aggregates in Infected Synovial Fluids From Joint Infections

Identification and Characterization of Planktonic Biofilm-Like Aggregates in Infected Synovial Fluids From Joint Infections

Human pathogens utilize host extracellular matrix proteins laminin and collagen for adhesion and invasion of the host

Bacterial vaginosis – a microbiological and immunological enigma

Contact Info

Product

Resources

About