Fibronectin and Factor VIII-Related Antigen in Acute Leukaemia

Klingemann, Hans‐G.; Kosukavak, Mustafa; Höfeler, H.; Havemann, K.

doi:10.1515/bchm2.1983.364.1.269

Cited by 16 publications

(2 citation statements)

References 6 publications

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“…Factors VIII and vWF was increased in AML and ALL patients. After chemotherapy, these factors reduced indicated as cancer cells decreased, they tend to decrease the factor VIII production by decreasing the procoagulants (17). The derangements of thrombotic markers were also observed in present investigation.…”

Section: Discussionsupporting

confidence: 77%

Hemostatic and thrombotic parameters in acure leukemia- A comparison of pre and post remission induction phase

Khan¹,

Masood

Bashir

et al. 2022

LMRJ

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This study was aimed to compare hemostatic, fibrinolytic and thrombotic parameters in pre and post induction chemotherapy in acute myeloid leukemia (AML) and acute lymphoid leukemia (ALL). A total of 110 diagnosed acute leukemia patients and 40 normal individuals were enrolled in the study. Questionnaire were filled and patients’ blood specimens were collected before the commencement and post induction chemotherapy. Prothrombin time (PT), activated partial thromboplastin time (aPTT), von Willebrand factor antigen (vWF Ag), fibrinogen level, factor VIIIc (FVIIIc), D-Dimers, fibrinogen degradation products (FDPs), anti-thrombin III (AT), lupus anticoagulant (LA), protein C (PC), protein S and diluted russel viper venom test (DRVVT) were conducted in all the participants. A significant rise was identified in post-induction levels of PT, aPTT, vWF Ag, fibrinogen and factor VIII in acute leukemia patients. Analysis of fibrinolytic markers indicated increased D-Dimers and plasminogen levels while the levels of alpha 2 anti-plasmin were reduced in pretreated patients. Thrombotic markers’ assessment showed increased levels of AT and LA while decreased level of PC in pretreated acute leukemia patients. It was concluded that remission induction chemotherapy, in acute leukemia patients, significantly affects the coagulation, fibrinolytic and thrombotic parameters.

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Section: Discussionsupporting

confidence: 77%

Hemostatic and thrombotic parameters in acure leukemia- A comparison of pre and post remission induction phase

Khan¹,

Masood

Bashir

et al. 2022

LMRJ

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“…This property was reported to cause the heterogeneity of the circulat ing FN in some pathological states. FN bound to fibrinogen, probably by the action of activated factor XIII, was detected by crossed immunoelectrophoresis in plasma of patients with acute leukemia (7). Using the same technique Reilly et al (8) found a slow migrating complex of FN either with fibrinogen antigen or, Correspondence to: K. Bykowska, Ph.…”

Section: Introductionmentioning

confidence: 99%

Effect of Proteolysis on Quantitation of Plasma Fibronectin Concentration by Two Immunoassays (Electroimmunoassay and Immunoturbidimetric Technique)

et al. 1985

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SummaryQuantitation of fibronectin (FN) concentration is strongly influenced by FN fragmentation with trypsin, kallikrein and plasmin. Digestion by trypsin and kallikrein leads to a progressive decline in FN detectability by the immunoturbidimetric technique to zero values but is associated with an increase in the height of rockets in the Laurell’s electroimmunoassay. Plasmin mediated FN fragmentation induces a strong overestimation of the FN content by the electroimmunoassay and, at very high enzyme concentrations, provokes an underestimation of FN by the immunoturbidimetric technique. The decline in FN reactivity in the immunoturbidimetric assay coincides with the disappearance of heavy fractions migrating only slightly faster than native FN in SDS-PAGE. The increase in the height of rockets in the electroimmunoassay is the highest when fractions of intermediate rate of migration prevail in the SDS-PAGE pattern. Concomitant use of these two immunoassays can distinguish native FN from its degraded form and may possibly provide a partial explanation for discrepancies in published studies on the concentration of circulating FN in various pathological states.

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Characterization of the molecular forms of fibronectin in fulminant hepatic failure

Almasio¹,

Hughes²,

Williams³

1986

Hepatology

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The plasma levels of the opsonic glycoprotein fibronectin are decreased in patients with fulminant hepatic failure, which may be an important factor in their impaired host-defense. Twenty-nine patients in fulminant hepatic failure were studied on admission, and the mean fibronectin level in Grade 0-2 encephalopathy was 82 micrograms per ml (range = 0 to 150) and in Grade 3-4 encephalopathy 61 micrograms per ml (range = 5 to 158) as compared to normal controls (268 micrograms per ml, range = 178 to 380, n = 62). No fibronectin degradation products could be detected in fulminant hepatic failure plasma by sodium dodecyl sulfate-gel electrophoresis on a polyacrylamide gradient (5 to 15%) followed by immunoblotting onto nitrocellulose with detection using a rabbit antihuman fibronectin antiserum visualized with a peroxidase conjugate. The plasma levels of the marker proteolytic enzyme cathepsin D were significantly elevated in fulminant hepatic failure (120 +/- 31 mU per ml per hr) as compared to the normal controls (18 +/- 2.1 mU per ml per hr, n = 10, p less than 0.01). Cross-immunoelectrophoresis of fulminant hepatic failure plasma for fibronectin on agarose plates gave an additional slower migrating peak in 15 of the 29 patients, as well as that of fibronectin, which corresponded to the fibronectin complex reported by other workers in leukemia. An intermediate gel containing antihuman fibrinogen demonstrated fibrinogen to be one component of this complex. Binding of other substances to fibronectin will reduce its apparent biological activity and may be the result of their lack of clearance by the damaged liver.

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Fibronectin and Factor VIII-Related Antigen in Acute Leukaemia

Cited by 16 publications

References 6 publications

Hemostatic and thrombotic parameters in acure leukemia- A comparison of pre and post remission induction phase

Hemostatic and thrombotic parameters in acure leukemia- A comparison of pre and post remission induction phase

Effect of Proteolysis on Quantitation of Plasma Fibronectin Concentration by Two Immunoassays (Electroimmunoassay and Immunoturbidimetric Technique)

Characterization of the molecular forms of fibronectin in fulminant hepatic failure

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