Fibronectin and Craniofacial Surgery

Al‐Qattan, Mohammad M.; Alshomer, Feras; Alqahtani, Abdullah S; Alhadlg, Ahmad

doi:10.1097/sap.0b013e3182858812

Cited by 6 publications

(2 citation statements)

References 73 publications

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“…It has been reported that FN promotes the migrating of neural crest cells to form the calvarial bone via regulating cell‐to‐cell attachment, which makes FN a promising tool in stem cell technology in light of craniofacial surgery. [ 49 ] FN is known to bind α 5 integrins (Itg α 5) via the RGD site located in FN. Notably, FN has been reported to play a significant role in osteogenic and chondrogenic differentiation, and has been recognized as a potent regulator of bone fracture healing.…”

Section: Resultsmentioning

confidence: 99%

Metabolic Response Modulations by Zwitterionic Hydrogels for Achieving Promoted Bone Regeneration

Li,

Yue,

Peng

et al. 2023

Adv Funct Materials

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Zwitterionic materials containing both cationic and anionic ligands have been reported to promote hydroxyapaptite (HA)‐mineralization. However, how zwitterionic microenvironment regulates osteogenesis of bone marrow mesenchymal stem cells (BMSCs) and bone regeneration remains elusive. Here, a strategy using zwitterionic (2‐(N‐3‐sulfopropyl‐N, N‐dimethyl ammonium) ethyl methacrylate/ Methacrylated gelatin (DMAPS/GelMA) hydrogel to promote osteogenic differentiation of BMSCs in vitro and enhance bone defect repair in vivo is developed. This zwitterionic hydrogel exhibits high stereo‐affinity of fibronectin III7‐10 (FnIII7‐10) and accelerates endogenous stem cell recruitment during bone regeneration. Then, the metabolic architecture of zwitterion‐guided osteogenesis is explored by using precise untargeted metabolomics, and the metabolic profile is mapped in the zwitterionic microenvironment. The zwitterion‐dependent energetic metabolic profile reveals that amino acids capable of promoting osteogenesis, such as proline and hydroxyproline, are enriched in zwitterionic extracellular matrix (ECM); whereas, fatty acids suggestting the inflammatory response, such as dinoprostone and prostaglandin h2, are enriched in either positive‐charged ECM or negative‐charged ECM. This zwitterionic ECM‐dependent metabolic landscape is the underlying mechanism of zwitterionic hydrogel‐promoted osteogenic differentiation of BMSCs. Therefore, the study provides a deeper contextual understanding of zwitterionic‐directed bone regeneration, leading to stereochemical principles of valuable functionalized biomaterial design.

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Section: Resultsmentioning

confidence: 99%

Metabolic Response Modulations by Zwitterionic Hydrogels for Achieving Promoted Bone Regeneration

Li,

Yue,

Peng

et al. 2023

Adv Funct Materials

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show abstract

“…Similarly, fibronectin, which is involved in mediating differentiation of the skull and migration of neural crest cells, works as a carrier for BMPs and used as an essential component in stem cell technology associated with craniofacial Al-Qattan et al, 2014, Brunskill et al, 2014. Engineering cranial neural crest cells is also a tool to treat diseases.…”

Section: Neural Crest Cells and Regenerative Medicinementioning

confidence: 99%

Contribution of cranial neural crest cells to mouse skull development

Wu¹,

Chen²,

Tian³

et al. 2017

Int. J. Dev. Biol.

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The mammalian skull vault is a highly regulated structure that evolutionally protects brain growth during vertebrate development. It consists of several membrane bones with different tissue origins (e.g. neural crest-derived frontal bone and mesoderm-derived parietal bone). Although membrane bones are formed through intramembranous ossification, the neural crest-derived frontal bone has superior capabilities for osteoblast activities and bone regeneration via TGF, BMP, Wnt, and FGF signaling pathways. Neural crest (NC) cells are multipotent, and once induced, will follow specific paths to migrate to different locations of the body where they give rise to a diverse array of cell types and tissues. Recent studies using genetic mouse models have greatly advanced our knowledge of NC cell induction, proliferation, migration and differentiation. Perturbations or disruptions of neural crest patterning lead to severe developmental defects or diseases. This review summarizes recent discoveries including novel functions of genes or signaling molecules that are capable of governing developmental processes of neural crest patterning, which may function as a gene regulatory network in controlling skull development. The proposed regulatory network will be important to understand how the signaling pathways and genes converge to regulate osteoblast activities and bone formation, which will be beneficial for the potential identification of molecular targets to prevent or alleviate human diseases or disorders involving defective neural crest development.

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Lamellipodia‐Mediated Osteoblast Haptotaxis Guided by Fibronectin Ligand Concentrations on a Multiplex Chip

Liu,

Feng,

Jeong

et al. 2024

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Skull morphogenesis is a complex, dynamic process involving two different germ layers and progressing to the coordinated, directional growth of individual bones. The mechanisms underlying directional growth toward the apex are not completely understood. Here, a microfluidic chip‐based approach is utilized to test whether calvarial osteoblast progenitors undergo haptotaxis on a gradient of Fibronectin1 (FN1) via lamellipodia. Mimicking the embryonic cranial mesenchyme's FN1 pattern, FN1 gradients is established in the chip using computer modeling and fluorescent labeling. Primary mouse calvarial osteoblast progenitors are plated in the chip along an array of segmented gradients of adsorbed FN1. The study performs single‐cell tracking and measures protrusive activity. Haptotaxis is observed at an intermediate FN1 concentration, with an average directional migration index (yFMI) of 0.07, showing a significant increase compared to the control average yFMI of ‐0.01. A significant increase in protrusive activity is observed during haptotaxis. Haptotaxis is an Arp2/3‐dependent, lamellipodia‐mediated process. Calvarial osteoblast progenitors treated with the Arp2/3 (Actin Related Protein 2/3 complex) inhibitor CK666 show significantly diminished haptotaxis, with an average yFMI of 0.01. Together, these results demonstrate haptotaxis on an FN1 gradient as a new mechanism in the apical expansion of calvarial osteoblast progenitors during development and shed light on the etiology of calvarial defects.

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Fibronectin and Craniofacial Surgery

Cited by 6 publications

References 73 publications

Metabolic Response Modulations by Zwitterionic Hydrogels for Achieving Promoted Bone Regeneration

Metabolic Response Modulations by Zwitterionic Hydrogels for Achieving Promoted Bone Regeneration

Contribution of cranial neural crest cells to mouse skull development

Lamellipodia‐Mediated Osteoblast Haptotaxis Guided by Fibronectin Ligand Concentrations on a Multiplex Chip

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