Fibronectin and Cell Attachment to Cell and Protein Resistant Polyelectrolyte Surfaces

Olenych, Scott G.; Moussallem, Maroun D.; Salloum, David S.; Schlenoff, Joseph B.; Keller, Thomas C. S.

doi:10.1021/bm050298r

Cited by 71 publications

(76 citation statements)

References 34 publications

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“…Layer-bylayer deposition provides an alternative strategy to fine tune the surface properties of polymeric scaffolds on the nanometer scale [182][183][184]. Recent studies demonstrate that one can easily control the cell behaviors on these self-assembled multilayers by simple manipulation of the deposition process [185][186][187][188][189][190]. However, polyelectrolyte multilayer studies are limited to planner surfaces and spherical particles.…”

Section: Nanofibrous Scaffolds By Electrospinningmentioning

confidence: 99%

Nanostructured materials for applications in drug delivery and tissue engineering

Goldberg

Langer

Jia

2007

Journal of Biomaterials Science, Polymer Edition

943

554

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Research in the areas of drug delivery and tissue engineering has witnessed tremendous progress in recent years due to their unlimited potential to improve human health. Meanwhile, the development of nanotechnology provides opportunities to characterize, manipulate and organize matter systematically at the nanometer scale. Biomaterials with nano-scale organizations have been used as controlled release reservoirs for drug delivery and artificial matrices for tissue engineering. Drug-delivery systems can be synthesized with controlled composition, shape, size and morphology. Their surface properties can be manipulated to increase solubility, immunocompatibility and cellular uptake. The limitations of current drug delivery systems include suboptimal bioavailability, limited effective targeting and potential cytotoxicity. Promising and versatile nano-scale drug-delivery systems include nanoparticles, nanocapsules, nanotubes, nanogels and dendrimers. They can be used to deliver both small-molecule drugs and various classes of biomacromolecules, such as peptides, proteins, plasmid DNA and synthetic oligodeoxynucleotides. Whereas traditional tissue-engineering scaffolds were based on hydrolytically degradable macroporous materials, current approaches emphasize the control over cell behaviors and tissue formation by nano-scale topography that closely mimics the natural extracellular matrix (ECM). The understanding that the natural ECM is a multifunctional nanocomposite motivated researchers to develop nanofibrous scaffolds through electrospinning or self-assembly. Nanocomposites containing nanocrystals have been shown to elicit active bone growth. Drug delivery and tissue engineering are closely related fields. In fact, tissue engineering can be viewed as a special case of drug delivery where the goal is to accomplish controlled delivery of mammalian cells. Controlled release of therapeutic factors in turn will enhance the efficacy of tissue engineering. From a materials point of view, both the drug-delivery vehicles and tissue-engineering scaffolds need to be biocompatible and biodegradable. The biological functions of encapsulated drugs and cells can be dramatically enhanced by designing biomaterials with controlled organizations at the nanometer scale. This review summarizes the most recent development in utilizing nanostructured materials for applications in drug delivery and tissue engineering.

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Section: Nanofibrous Scaffolds By Electrospinningmentioning

confidence: 99%

Nanostructured materials for applications in drug delivery and tissue engineering

Goldberg

Langer

Jia

2007

Journal of Biomaterials Science, Polymer Edition

943

554

View full text Add to dashboard Cite

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“…If the polyelectrolytes are in the less-ionized state due to the deposition pH, the layers will be hydrated, thick and soft. [129][130][131][132][133] (See Figure 5 .) Polyelectrolyte multilayers have been used to create functional substrates for growth of endothelial, [ 134 ] muscle, [ 135 ] nerve, [ 136 ] bone, [ 137 ] cartilage, [ 138 ] hepatocyte, [ 139 ] and stem cells. [ 140,141 ] From these studies it is apparent that stiff, thin layers promote adhesion much better than soft, hydrated fi lms.…”

Section: Sweet Spots: Glycomimetics In Tissue Engineeringmentioning

confidence: 99%

Engineering the Extracellular Environment: Strategies for Building 2D and 3D Cellular Structures

et al. 2010

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Cell fate is regulated by extracellular environmental signals. Receptor specific interaction of the cell with proteins, glycans, soluble factors as well as neighboring cells can steer cells towards proliferation, differentiation, apoptosis or migration. In this review, approaches to build cellular structures by engineering aspects of the extracellular environment are described. These methods include non-specific modifications to control the wettability and stiffness of surfaces using self-assembled monolayers (SAMs) and polyelectrolyte multilayers (PEMs) as well as methods where the temporal activation and spatial distribution of adhesion ligands is controlled. Building on these techniques, construction of two-dimensional cell sheets using temperature sensitive polymers or electrochemical dissolution is described together with current applications of these grafts in the clinical arena. Finally, methods to pattern cells in three-dimensions as well as to functionalize the 3D environment with biologic motifs take us one step closer to being able to engineer multicellular tissues and organs.

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“…This is in contrast to the pTTh-SP where the nitro groups will contribute to hydrophilicity. The reduction in hydrophobicity of the MC form is attributed to the zwitterionic nature of the MC molecule, which has previously been shown to also reduce protein adhesion [33][34].…”

Section: Protein Adhesionmentioning

confidence: 94%

Optical switching of protein interactions on photosensitive–electroactive polymers measured by atomic force microscopy

et al. 2013

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The ability to switch the physico-chemical properties of conducting polymers opens up new possibilities for a range of applications. Appropriately functionalised materials can provide routes to multi-modal switching, for example, in response to light and/or electrochemical stimuli. This capability is important in the field of bionics wherein remote and temporal control of the properties of materials is becoming attractive. The ability to actuate a film via photonic stimuli is particularly interesting as it facilitates the modulation of interactions between host binding sites and potential guest molecules. In this work, we studied two different polyterthiophenes: one was functionalised with a spiropyran photoswitch (pTTh-SP) and the second with a nonphotoswitchable methyl acetate moiety (pTTh-MA). These substrates were exposed to several cycles of illumination with light of different wavelengths and the resulting effect studied with UV-vis spectroscopy, contact angle and atomic force microscopy (AFM). The AFM tips were chemically activated with fibronectin (FN) and the adhesion force of the protein to the polymeric surface was measured. The pTTh-MA (no SP incorporated) showed a slightly higher average maximum adhesion (0.96 ± 0.14 nN) than the modified pTTh-SP surface (0.77 ± 0.08 nN), but after exposure of the pTTh-SP polymer to UV, the average maximum adhesion of the pTTh-MC (merocyanine form) was significantly smaller (0.49 ± 0.06 nN) than both the pTTh-MA and pTTh-SP. In addition, the tip-sample separation distances of the adhesive interactions are indicative of the FN interaction occurring over a distance more closely related to the average dimensions of its compact conformation. The results suggest that surface energy and hydrophobic forces are predominant in determining the protein adhesion to the films studied and that this effect can be photonically tuned. By extension, this further implies that it should be possible to obtain a degree of spatial and temporal control of the surface binding behaviour of certain proteins with these functionalised surfaces through photo-activation/ deactivation, which, in principle, should facilitate patterned growth behaviour (e.g. using masks or directional illumination) or photocontrol of protein uptake and release. AbstractThe ability to switch the physico-chemical properties of conducting polymers opens up new possibilities for a range of applications. Appropriately functionalised materials can provide routes to multi-modal switching, for example, in response light and/or electrochemical stimuli. This capability is important in the field of bionics wherein remote and temporal control of the properties of materials is becoming attractive. The ability to actuate a film via photonic stimuli is particularly interesting as it facilitates the modulation of interactions between surface host binding sites and potential guest molecules. In this work, we studied two different poly-terthiophenes: one was functionalized with a spiropyran photoswitch (pTTh-SP) and the sec...

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Fibronectin and Cell Attachment to Cell and Protein Resistant Polyelectrolyte Surfaces

Cited by 71 publications

References 34 publications

Nanostructured materials for applications in drug delivery and tissue engineering

Nanostructured materials for applications in drug delivery and tissue engineering

Engineering the Extracellular Environment: Strategies for Building 2D and 3D Cellular Structures

Optical switching of protein interactions on photosensitive–electroactive polymers measured by atomic force microscopy

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